Differentiation potential and functional properties of a CD34 ‑CD133+ subpopulation of endothelial progenitor cells.

Differentiation potential and functional properties of a CD34‑CD133+ subpopulation of endothelial progenitor cells. Mol Med Rep. 2019 Nov 20;: Authors: Bachelier K, Bergholz C, Friedrich EB Abstract Endothelial progenitor cells (EPCs) promote angiogenesis and play an important role in myocardial and vascular repair after ischemia and infarction. EPCs consist of different subpopulations including CD34‑CD133+ EPCs, which are precursors of more mature CD34+CD133+ EPCs and functionally more active in terms of homing and endothelial regeneration. In the present study we analyzed the functional and differentiation abilities of CD34‑CD133+ EPCs. Isolation of EPC populations (CD34+CD133+, CD34‑CD133+) were performed by specific multi‑step magnetic depletion. After specific stimulation a significant higher adhesive and migrative capacity of CD34‑CD133+ cells could be detected compared to CD34+CD133+ cells (P<0.001, respectively). Next to this finding, not only significantly higher rates of proliferation (P<0.005) were detected among CD34‑CD133+ cells, but also a higher potential of cell‑differentiation capacity into other cell types. Next to a significant increase of CD34‑CD133+ EPCs differentiating into a fibroblast cell‑type (P<0.001), an enhancement into a hepatocytic cell‑type (P=0.033) and a neural cell‑type (P=0.016) could be measured in contrast to CD34+CD133+ cells. On the other hand, there was no sign...
Source: Molecular Medicine Reports - Category: Molecular Biology Tags: Mol Med Rep Source Type: research