Leukemia inhibitory factor induces corticotropin-releasing hormone in mouse trophoblast stem cells.

In this study, we examined the effect of pro-inflammatory cytokine LIF on the induction of CRH in mouse trophoblast stem cells (mTSCs). During differentiation, the CRH levels in mTSCs gradually increased. On days 3 and 5 after LIF supplementation, Crh expression in the differentiated mTSCs was significantly increased with LIF treatment than those without LIF treatment. Moreover, the CRH concentration in the culture media increased. Thereafter, we examined the contribution of the downstream pathways of LIF to CRH induction in differentiated mTSCs. The LIF-induced upregulation of CRH was attenuated by inhibition of PI3K/AKT and MAPK phosphorylation but not by inhibition of JAK/STAT3. Therefore, in mTSCs, LIF increased Crh expression through activation of the PI3K/AKT and MAPK pathways but not by the JAK/STAT3 pathway. The present study suggests that mTSC is an ideal in vitro model for studying regulation and function of placental CRH.
PMID: 31740000 [PubMed - as supplied by publisher]

Source: Biochemical and Biophysical Research communications - November 14, 2019 Category: Biochemistry Authors: Wang H, Tsukada T, Shimada H, Sakata-Haga H, Iida Y, Zhang S, Shoji H, Hatta T Tags: Biochem Biophys Res Commun Source Type: research