Development of a filtration-based SERS mapping platform for specific screening of Salmonella enterica serovar Enteritidis.

This study demonstrates the capability of the filtration-based SERS platform for detecting Salmonella Enteritidis in various aqueous matrices such as distilled water and rinsing water from fresh produce with high selectivity and sensitivity. Graphical abstract. PMID: 31745614 [PubMed - as supplied by publisher]
Source: Analytical and Bioanalytical Chemistry - Category: Chemistry Authors: Tags: Anal Bioanal Chem Source Type: research

Related Links:

In this study, our aim was to evaluate ClO2 precursors, sodium chlorite and citric acid, under disparate but applicable conditions. First, we modeled production of ClO2 from the powdered precursors with three different masses, sample ID 1, 2, and 3, at room (18 °C) and refrigeration (4 °C) temperatures. Second, we investigated the effects of a water reservoir on ClO2 production. Third, we assessed collection method issues that may impact reported ClO2 values. Finally, we evaluated the precursor's efficacy against three common foodborne pathogens: Escherichia coli O157:H7, Listeria monocytogenes, and Salmonella ...
Source: Innovative Food Science and Emerging Technologies - Category: Food Science Source Type: research
This study provides scientific data to better understand the risks of potential VBNC foodborne pathogens during fresh produce washing.
Source: Food Control - Category: Food Science Source Type: research
The aim of this study was the assessment of the effect of time exposure, temperature, distance, and organic contaminants on radiant catalytic ionization (RCI) microbicidal effectiveness. The number of all examined bacteria decreased together with time exposure of RCI. The lowest recovery was obtained, both from the rubber surface (6.36 log CFU × cm–2) and steel (6.04 log CFU × cm–2) in the case of Escherichia coli O157:H7. On the other hand, Staphylococcus aureus was isolated in the largest number (rubber: 7.88 log CFU × cm–2, steel: 7.79 log CFU × cm–2). Among the tested env...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Abstract We evaluated the bactericidal efficacy of the simultaneous application of ultraviolet-A (UV-A) irradiation and fumaric acid (FA) against Escherichia coli O157:H7, Salmonella enterica serovar Typhimurium, and Listeria monocytogenes in apple juice and as well as investigated the effects of this treatment on product quality. Further, we elucidated the mechanisms underlying their synergistic bactericidal action. Simultaneous UV-A light irradiation and 0.1% FA treatment for 30 min resulted in 6.65-, 6.27-, and 6.49-log CFU/ml reductions in E. coli O157:H7, S. Typhimurium, and L. monocytogenes, respectivel...
Source: Food Microbiology - Category: Food Science Authors: Tags: Food Microbiol Source Type: research
Publication date: Available online 7 January 2020Source: Sensors and Actuators B: ChemicalAuthor(s): Sirirat Wachiralurpan, Kosum Chansiri, Peter A. LieberzeitAbstractCurrent methods for identifying Listeria monocytogenes are both time and labor intensive. However, it is highly desirable to detect it rapidly and reliably to prevent and/or identify contamination of foodstuff. Herein we propose a mass-sensitive sensor operating at elevated temperatures, 50-55 °C, for that purpose. Sensitive and selective detection relies on distinguishing genes of genomic extract of L. monocytogenes. A thiol-modified ssDNA probe d...
Source: Sensors and Actuators B: Chemical - Category: Chemistry Source Type: research
In this study, we established a whole-cell method for the identification of foodborne pathogenic bacteria, using MALDI-TOF MS and principal component analysis (PCA), which did not use protein extractions or expensive protein databases. Thirty strains comprising six common foodborne pathogenic bacteria, namely, Shigella flexneri, Escherichia coli, Staphylococcus aureus, Salmonella enteritidis, Pseudomonas aeruginosa, and Listeria monocytogenes were analyzed using MALDI-TOF MS. The culture time, matrix, and spotting method were optimized based on peak intensity and deviation. A PCA was performed to analyze the mass spectrome...
Source: Analytical Biochemistry - Category: Biochemistry Authors: Tags: Anal Biochem Source Type: research
This study investigated the antimicrobial activity of pecan shell extracts as effected by the type of cultivar and the method of extraction against various foodborne bacterial pathogens. Defatted shell powders of 19 different pecan cultivars were subjected to aqueous and ethanolic extraction (1:20 w/v) procedures, respectively. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of lyophilized pecan shell extracts dissolved in deionized water containing 5% DMSO (v/v) were determined against multiple strains of Listeria monocytogenes, Salmonella enterica, and Escherichia coli O157:H7. The...
Source: Food Control - Category: Food Science Source Type: research
This study investigated the antimicrobial capacity of yeasts, Saccharomyces cerevisiae and Pichia kluyveri, previously isolated from fermented foods (indigenous beverage, kefir and cocoa) against the adhesion of foodborne pathogens to Caco-2 cells. Co-aggregation of yeasts with pathogens and were evaluated by quantitative analysis and using scanning electron and laser confocal microscopies. All yeasts strains were able to co-aggregate with the tested pathogens, however, this activity was strain-dependent. The inhibition tests showed that the adhesion of Escherichia coli EPEC, Listeria monocytogenes and Salmonella Enterit...
Source: Microbial Pathogenesis - Category: Infectious Diseases Source Type: research
Authors: Hernández-Aquino S, Miranda-Romero LA, Fujikawa H, Maldonado-Simán EJ, Alarcón-Zuñiga B Abstract Lactic Acid Bacteria (LAB) are generally recognized as safe. It has been used to increase the shelf-life of fermented products, and its antimicrobial action is based on the metabolites secretions, such as lactic acid, hydrogen peroxide, reuterin, bacteriocins and the like-bacteriocins substances. It has been proven that LAB are able to inhibit deteriorating bacteria of raw meat, but improper handling of live cultures could lead to spoilage. So, the use of their bacteriocins, smal...
Source: Biocontrol Science - Category: Microbiology Tags: Biocontrol Sci Source Type: research
AbstractIn the present study, we evaluated the degree of contamination of fresh vegetables, cheeses and jellies from disaster area in Brazil with bacteria and enteric viruses. Food samples (n = 350) were tested forEscherichia coli,Salmonella spp.,Listeria monocytogenes,Staphylococcus spp., and enteric viruses (rotavirus A (RVA), human adenovirus (HAdV), hepatitis A virus (HAV), and human norovirus (HNoV).E. coli was present in 56% of the samples,Salmonella spp. was present in 14% of the samples,L. monocytogenes andStaphylococcus spp. (coagulase-positive) were present in 36% of the samples. The enteric viruses RVA and HAdV ...
Source: Archives of Virology - Category: Virology Source Type: research
More News: Chemistry | Gastroenteritis | International Medicine & Public Health | Listeria | Nanotechnology | Salmonella | Study