Enhancing medial layer recellularization of tissue-engineered blood vessels using radial microchannels.
Enhancing medial layer recellularization of tissue-engineered blood vessels using radial microchannels.
Regen Med. 2019 Nov 20;:
Authors: Eufrásio-da-Silva T, Ruiz-Hernandez E, O'Dwyer J, Picazo-Frutos D, Duffy GP, Murphy BP
Abstract
Aim: Cell repopulation of tissue-engineered vascular grafts (TEVGs) from decellularized arterial scaffolds is limited by dense concentric tunica media layers which impede cells migrating radially between the layers. We aimed to develop and validate a new microneedle device to modify decellularized carotid arteries with radial microchannels to enhance medial layer repopulation. Material & methods: Modified decellularized porcine arteries were seeded with rat mesenchymal stem cells using either standard longitudinal injection, or a dual vacuum-perfusion bioreactor. Mechanical tests were used to assess the arterial integrity following modification. Results & conclusion: The method herein achieved radial recellularization of arteries in vitro without significant loss of mechanical integrity, Thus, we report a novel method for successful radial repopulation of decellularized carotid artery-based tissue-engineered vascular grafts.
PMID: 31746270 [PubMed - as supplied by publisher]
Source: Regenerative Medicine - Category: Genetics & Stem Cells Authors: Eufrásio-da-Silva T, Ruiz-Hernandez E, O'Dwyer J, Picazo-Frutos D, Duffy GP, Murphy BP Tags: Regen Med Source Type: research