Anticholinergic and Antioxidant Activities of Usnic Acid-An Activity-Structure Insight

Publication date: Available online 20 November 2019Source: Toxicology ReportsAuthor(s): Kader Cetin Cakmak, İlhami GülçinAbstractUsnic acid, as active dibenzofuran derivative, isolated and characterized from some lichen species. The aim of manuscript was to evaluate antioxidant, anticholinergic and antidiabetic potentials of usnic acid as an important natural product. Antioxidant profile of usnic acid determined by eight distinguishes bioanalytical antioxidant methods. The antioxidative properties of usnic acid were performed using by several bioanalytical assays. 1,1-diphenyl-2-picrylhydrazyl (DPPH·), 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS•+), superoxide anion radical (O2•-) and N,N-dimethyl-p-phenylenediamine (DMPD•+) scavenging activities, cupric ion (Cu2+), ferric ion (Fe3+) and Fe3+-TPTZ reducing abilities and ferrous ion (Fe3+) chelating activity. Usnic acid was found as potent DPPH· (IC50: 49.50 μg/mL), DMPD•+ (IC50: 33.00 μg/mL), O2•- (IC50: 18.68 μg/mL), and ABTS•+ (IC50: 10.41 μg/mL) scavenging effects. Also, the inhibition effects of usnic acid were tested against some metabolic enzymes including acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) linked to neurodegenerative diseases. Both enzymes play important roles in cholinergic transmission by hydrolyzing the neurotransmitter acetylcholine in cholinergic synapses, central nervous system, neuromuscular junctions and autonomic ganglia. Their inhibitors...
Source: Toxicology Reports - Category: Toxicology Source Type: research