Multiplexed Digital Detection of B-cell Acute Lymphoblastic Leukemia Fusion Transcripts Using the NanoString nCounter System

In this study, we used NanoString technology to design, validate, and evaluate a multiplex panel for the detection of B-ALL fusion transcripts. Fifty-one B-ALL fusion transcripts reported in children in the literature were included in the design of the NanoString panel. Twenty-six fusion transcripts were validated using 64 positive-control samples and 74 negative-control samples with 100% sensitivity and 99% specificity in comparison to RT-PCR.

Source: Journal of Molecular Diagnostics - November 12, 2019 Category: Pathology Authors: Yunan Zhong, Kassa Beimnet, Zaman Alli, Anthony Arnoldo, Paul E. Kowalski, Gino R. Somers, Cynthia Hawkins, Mohamed Abdelhaleem Source Type: research