Molecular characterization and RSV Co-infection of Nicotiana benthamiana with three distinct begomoviruses

Publication date: Available online 20 November 2019Source: MethodsAuthor(s): Muhammad Arif, Sagheer Atta, Muhammad Amjad Bashir, Ansar Hussain, Muhammad Ifnan Khan, Shahid Farooq, Abdul Hanan, Saif ul Islam, Ummad ud din Umar, Mehran Khan, Wenzhong Lin, Mohamed Hashem, Saad Alamri, Zujian WuAbstractGeminiviruses constitute a family of plant viruses with characteristic twinned quasi-icosahedral virions and a small circular DNA genome. Geminiviruses, especially begomoviruses, cause substantial economic losses in tropical and subtropical regions globally. Geminiviruses use the host’s transcriptional mechanisms to synthesize their mRNAs. They are considered as an attractive model to understand the transcription mechanism of their host plants. Experiments were conducted to identify transcriptional start sites (TSSs) of the three begomoviruses, i.e., Cotton leaf curl Multan virus (CLCuMuV), Corchorus yellow vein virus (CoYVV), and Ramie mosaic virus (RamV). We first rub-inoculated Rice stripe tenuivirus (RSV), a segmented negative-sense RNA virus that uses cap-snatching to produce capped viral mRNAs, into N. benthamiana. After the inoculation, RSV-infected N. benthamiana were super-infected by CoYVV, CLCuMuV, or RamV, respectively. The capped-RNA leaders snatched by RSV were obtained by determining the 5′-ends of RSV mRNA with high throughput sequencing. Afterwards, snatched capped-RNA leaders of RSV were mapped onto the genome of each begomovirus and those matching the begomov...
Source: Methods - Category: Molecular Biology Source Type: research