Degradation of polysaccharides from Sargassum fusiforme using UV/H2O2 and its effects on structural characteristics

Publication date: Available online 19 November 2019Source: Carbohydrate PolymersAuthor(s): Xiaoyong Chen, Ruifen Zhang, Yizhou Li, Xiong Li, Lijun You, Viktoryia Kulikouskaya, Kseniya HileuskayaAbstractThe depolymerization effect of UV/H2O2 on the polysaccharides from Sargassum fusiforme (PSF), a brown algae, were studied. The structural changes of PSF before and after UV/H2O2 treatment were analyzed, and molecular weight changes during in vitro digestion were determined. Results indicated that the molecular weight of PSF was reduced from ∼289 to ∼12.6 kDa within 2 h with UV/150 mmol/L H2O2, and the depolymerization effect of UV/H2O2 was significantly higher than that of UV or H2O2 alone. In addition, the UV/H2O2 treatment had a high recovery rate of total sugar (93.54%) and clearance rate of protein (76.34%). The monosaccharide composition showed that UV/H2O2 treatment could increase the mole percentage of mannose (37.44%) and decrease the mole percentage of fucose (14.88%). The helix-coil transition, X-ray diffraction (XRD) and atomic force microscopy (AFM) imaging showed that the UV/H2O2 treatment depolymerized PSF. Rheological studies indicated that PSF with UV/H2O2 treatment had lower viscosity. In vitro digestion showed that PSF was minimally digested with the in vitro gastrointestinal tract simulation, but PSF with UV/H2O2 treatment could be digested in the low acid environment in the simulated gastric juice, but was minimally digested with the simulated inte...
Source: Carbohydrate Polymers - Category: Biomedical Science Source Type: research