Genes, Vol. 10, Pages 949: Chalcone Synthase-Encoding AeCHS is Involved in Normal Petal Coloration in Actinidia eriantha

Genes, Vol. 10, Pages 949: Chalcone Synthase-Encoding AeCHS is Involved in Normal Petal Coloration in Actinidia eriantha
Genes doi: 10.3390/genes10120949
Authors:
Li
Cui
Qi
Qiao
Lin
Zhong
Hu
Fang

Studies on anthocyanin biosynthesis have been mainly concentrated on the fruit, whereas few have focused the mechanism of flower coloration in kiwifruit. Here, we report that the structural gene, AeCHS, is involved in anthocyanin accumulation and indispensable for normal petal coloration in Actinidia eriantha. Petals from three different species including Actinidia eriantha (red petals), Actinidia hemsleyana (light pink petals) and Actinidia arguta (white petals) were selected for anthocyanin determination and gene expression analysis. The anthocyanin components in A. eriantha were significantly higher than in A. hemsleyana or A. arguta. Consistently, gene expression profiles suggested that AeCHS expression in A. eriantha was higher than in A. hemsleyana or A. arguta. Cluster analysis showed that AeCHS was clustered into a single group and distinctly separated from other genes, indicating the expression pattern of AeCHS gene was different from any other. Additionally, correlation analysis revealed AeCHS expression significantly correlated with anthocyanin content. The complete coding sequence of AeCHS was cloned from petals of A. eriantha ‘Zaoxu’, showing the length of AeCHS was 1170 bp encoding a protein of 389 amino acids. A...

Source: Genes - November 19, 2019 Category: Genetics & Stem Cells Authors: Li Cui Qi Qiao Lin Zhong Hu Fang Tags: Article Source Type: research