Viruses, Vol. 11, Pages 1079: Identification of B-Cell Epitopes with Potential to Serologicaly Discrimnate Dengue from Zika Infections

Viruses, Vol. 11, Pages 1079: Identification of B-Cell Epitopes with Potential to Serologicaly Discrimnate Dengue from Zika Infections Viruses doi: 10.3390/v11111079 Authors: Alice F. Versiani Raissa Prado Rocha Tiago A. O. Mendes Glauco C. Pereira Jordana Graziella A. Coelho dos Reis Daniella C. Bartholomeu Flávio G. da Fonseca Dengue is currently one of the most important arbovirus infections worldwide. Early diagnosis is important for disease outcome, particularly for those afflicted with the severe forms of infection. The goal of this work was to identify conserved and polymorphic linear B-cell Dengue virus (DENV) epitopes that could be used for diagnostic purposes. To this end, we aligned the predicted viral proteome of the four DENV serotype and performed in silico B-cell epitope mapping. We developed a script in Perl integrating alignment and prediction information to identify potential serotype-specific epitopes. We excluded epitopes that were similarly present in the yellow fever and zika viruses’ proteomes. A total of 15 polymorphic and nine conserved peptides among DENV serotypes were selected. Peptides were spotted on cellulose membranes and tested against sera from rabbits that were monoinfected with each DENV serotype. Although serotype-specific peptides failed to recognize any sera, three conserved peptides were recognized by all anti-dengue sera and were included on an ELISA test employing a well-characterized human sera ba...
Source: Viruses - Category: Virology Authors: Tags: Article Source Type: research

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Abstract Genus Flavivirus, which includes 53 virus species, is the leading cause of arthropod-borne diseases in humans. Diagnosis of these viral diseases is complicated by their overlapping epidemiology and clinical manifestations, and the fact that cross-reactive antibody responses are frequently elicited by individuals in response to infection. We developed a bead-based immunoassay to concomitantly profile the isotype and subclass of antibody responses (five isotypes and four subclasses) in parallel with specificity against multiple antigens. Our panel included 22 envelope (E) and non-structural 1 (NS1) proteins...
Source: Journal of Immunological Methods - Category: Allergy & Immunology Authors: Tags: J Immunol Methods Source Type: research
Zika virus (ZIKV) is an emerging arbovirus belonging to the genus Flavivirus, family Flaviviridae, which includes other viruses relevant to public health, such as dengue (DENV), yellow fever (YFV) and West Nile (WNV) viruses. Although ZIKV infection in humans is often characterized as a self-limiting disease 1,2, infections during pregnancy can lead to fetal malformations and neurodevelopment abnormalities 3 –5.
Source: Journal of Infection - Category: Infectious Diseases Authors: Source Type: research
Abstract Infections by flaviviruses, such as Dengue, West Nile, Yellow Fever and Zika viruses, represent a growing risk for global health. There are vaccines only for few flaviviruses while no effective treatments are available. Flaviviruses share epidemiological, structural, and ecologic features and often different viruses can co-infect the same host. Therefore, the identification of broad-spectrum inhibitors is highly desirable either for known flaviviruses or for viruses that likely will emerge in the future. Strategies targeting both virus and host factors have been pursued to identify broad-spectrum antiflav...
Source: ChemMedChem - Category: Chemistry Authors: Tags: ChemMedChem Source Type: research
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Source: Journal of Virological Methods - Category: Virology Authors: Tags: J Virol Methods Source Type: research
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Source: Infection, Genetics and Evolution - Category: Genetics & Stem Cells Authors: Tags: Infect Genet Evol Source Type: research
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Source: International Journal of Infectious Diseases - Category: Infectious Diseases Authors: Tags: 0631 Source Type: research
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Source: Acta Tropica - Category: Infectious Diseases Authors: Tags: Acta Trop Source Type: research
In the last decades, a number of infectious viruses have emerged from wildlife or re-emerged, generating serious threats to the global health and to the economy worldwide. Ebola and Marburg hemorrhagic fevers, Lassa fever, Dengue fever, Yellow fever, West Nile fever, Zika, and Chikungunya vector-borne diseases, Swine flu, Severe acute respiratory syndrome (SARS), Middle East respiratory syndrome (MERS), and the recent Coronavirus disease 2019 (COVID-19) are examples of zoonoses that have spread throughout the globe with such a significant impact on public health that the scientific community has been called for a rapid int...
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research
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Source: Molecules - Category: Chemistry Authors: Tags: Article Source Type: research
by Shuzhen Sim, Lee Ching Ng, Steve W. Lindsay, Anne L. Wilson Vector-borne diseases are a major cause of morbidity and mortality worldwide.Aedes-borne diseases, in particular, including dengue, chikungunya, yellow fever, and Zika, are increasing at an alarming rate due to urbanisation, population movement, weak vector control programmes, and climate change. The World Health Organization calls for strengthening of vector control programmes in line with the Global Vector Control Response (GVCR) strategy, and many vector control programmes are transitioning to this new approach. The Singapore dengue control programme, situa...
Source: PLoS Neglected Tropical Diseases - Category: Tropical Medicine Authors: Source Type: research
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