Viruses, Vol. 11, Pages 1079: Identification of B-Cell Epitopes with Potential to Serologicaly Discrimnate Dengue from Zika Infections

Viruses, Vol. 11, Pages 1079: Identification of B-Cell Epitopes with Potential to Serologicaly Discrimnate Dengue from Zika Infections Viruses doi: 10.3390/v11111079 Authors: Alice F. Versiani Raissa Prado Rocha Tiago A. O. Mendes Glauco C. Pereira Jordana Graziella A. Coelho dos Reis Daniella C. Bartholomeu Flávio G. da Fonseca Dengue is currently one of the most important arbovirus infections worldwide. Early diagnosis is important for disease outcome, particularly for those afflicted with the severe forms of infection. The goal of this work was to identify conserved and polymorphic linear B-cell Dengue virus (DENV) epitopes that could be used for diagnostic purposes. To this end, we aligned the predicted viral proteome of the four DENV serotype and performed in silico B-cell epitope mapping. We developed a script in Perl integrating alignment and prediction information to identify potential serotype-specific epitopes. We excluded epitopes that were similarly present in the yellow fever and zika viruses’ proteomes. A total of 15 polymorphic and nine conserved peptides among DENV serotypes were selected. Peptides were spotted on cellulose membranes and tested against sera from rabbits that were monoinfected with each DENV serotype. Although serotype-specific peptides failed to recognize any sera, three conserved peptides were recognized by all anti-dengue sera and were included on an ELISA test employing a well-characterized human sera bank...
Source: Viruses - Category: Virology Authors: Tags: Article Source Type: research