Receptor tyrosine kinase inhibitors block proliferation of TGEV mainly through p38 mitogen-activated protein kinase pathways

Publication date: Available online 18 November 2019Source: Antiviral ResearchAuthor(s): Wanyu Dong, Wenting Xie, Yunbo Liu, Baokun Sui, Hao Zhang, Liran Liu, Yubei Tan, Xiaohan Tong, Zhen F. Fu, Ping Yin, Liurong Fang, Guiqing PengAbstractEmerging coronaviruses (CoVs) primarily cause severe gastroenteric or respiratory diseases in humans and animals, and no approved therapeutics are currently available. Here, A9, a receptor tyrosine kinase inhibitor (RTKI) of the tyrphostin class, is identified as a robust inhibitor of transmissible gastroenteritis virus (TGEV) infection in cell-based assays. Moreover, A9 exhibited potent antiviral activity against the replication of various CoVs, including murine hepatitis virus (MHV), porcine epidemic diarrhea virus (PEDV) and feline infectious peritonitis virus (FIPV). We further performed a comparative phosphoproteomic analysis to investigate the mechanism of action of A9 against TGEV infection in vitro. We specifically identified p38 and JNK1, which are the downstream molecules of receptor tyrosine kinases (RTKs) required for efficient TGEV replication, as A9 targets through plaque assays, qRT-PCR and Western blotting assays. p38 and JNK1 inhibitors and RNA interference further showed that the inhibitory activity of A9 against TGEV infection was mainly mediated by the p38 mitogen-activated protein kinase (MAPK) signaling pathway. All these findings indicated that the RTKI A9 directly inhibits TGEV replication and that its inhibitory acti...
Source: Antiviral Therapy - Category: Virology Source Type: research

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Source: Reuters: Health - Category: Consumer Health News Tags: healthNews Source Type: news
(Natural News) Every time you turn on the news, there always seems to be a new deadly disease on the loose. These reports should be taken seriously. A pandemic can occur at any time and when it does, people’s lives will drastically change. People often interchange pandemic and epidemic, but they’re not quite the same....
Source: NaturalNews.com - Category: Consumer Health News Source Type: news
Authors: Clipa A, Şuţa M, Dumitru E, Muflic L PMID: 31803280 [PubMed]
Source: Journal of Medicine and Life - Category: General Medicine Tags: J Med Life Source Type: research
This study investigated the interaction of synthesized immunogenic ‘a’ determinant (aD) region of hepatitis B virus surface antigen (HBsAg) with PAMAM G4 and anti-HBsAg antibody, as a potential bio-nanogate for anti-HBsAg detection. The aD peptide fused with maltose binding protein (MBP), was confirmed with Western blotting. Nano-Differential Scanning Fluorimetry (nano-DSF) study revealed that the interaction of MBP-aD with anti-HBsAg indicated a higher thermal stability as compared to its interaction with PAMAM G4. Electrochemical impedance spectroscopy showed that a higher binding constant of MBP-aD interacti...
Source: Colloids and Surfaces B: Biointerfaces - Category: Biochemistry Source Type: research
Dana Carpender, friend and author of many low-carb cookbooks, provided this review of my new Revised &Expanded Wheat Belly, below. For more of Dana’s signature wit and conversation, you can join her on her engaging Facebook page “Hold the toast press” or visit her Amazon page that lists all her wonderful low-carb cookbooks. It’s funny how things happen. Nine years ago I had already been eating a low carbohydrate diet for 16 years. During that time I had occasionally eaten low carb tortillas and low carb bread. These things were hardly a staple of my diet, but I kept ’em around for the occ...
Source: Wheat Belly Blog - Category: Cardiology Authors: Tags: Open autoimmune joint pain wheat belly Source Type: blogs
Resveratrol Protects the Myocardium in Sepsis by Activating the Phosphatidylinositol 3-Kinases (PI3K)/AKT/Mammalian Target of Rapamycin (mTOR) Pathway and Inhibiting the Nuclear Factor-κB (NF-κB) Signaling Pathway. Med Sci Monit. 2019 Dec 06;25:9290-9298 Authors: Shang X, Lin K, Yu R, Zhu P, Zhang Y, Wang L, Xu J, Chen K Abstract BACKGROUND Sepsis combined with myocardial injury is an important cause of septic shock and multiple organ failure. However, the molecular mechanism of sepsis-induced myocardial dysfunction has not yet been thoroughly studied. Resveratrol has been an important rese...
Source: Medical Science Monitor - Category: Research Tags: Med Sci Monit Source Type: research
Publication date: Available online 7 December 2019Source: Saudi Pharmaceutical JournalAuthor(s): Khalid A. Alburikan, Hatem A. AbuelizzAbstractBackgroundMiddle East Respiratory Syndrome (MERS) is a respiratory disease caused by a novel coronavirus that was identified in 2012 in Saudi Arabia. It is associated with significant mortality and morbidity. We identified factors associated with the Middle East Respiratory Syndrome-Coronavirus (MERS‐CoV) infection among suspected cases presented with sign and symptoms of upper respiratory infection or exposure to the virus. We also looked at the impact of medication history on vi...
Source: Saudi Pharmaceutical Journal - Category: Drugs & Pharmacology Source Type: research
CONCLUSION: Enterococcus spp. with susceptibility to limited antibiotics was one of the main pathogens in Korean IAIs, along with E. coli and Klebsiella spp., which were highly susceptible to imipenem, amikacin, and piperacillin/tazobactam. Meanwhile, the low susceptibilities of E. coli or Klebsiella spp. to amoxicillin/clavulanate, advanced-generation cephalosporins, and ciprofloxacin should be considered when determining empirical antibiotic therapy in clinical practice. PMID: 31808326 [PubMed - in process]
Source: Journal of Korean Medical Science - Category: Biomedical Science Tags: J Korean Med Sci Source Type: research
Publication date: Available online 6 December 2019Source: Antiviral ResearchAuthor(s): Andrew M. Skidmore, Robert S. Adcock, Colleen B. Jonsson, Jennifer E. Golden, Dong-Hoon ChungAbstractVenezuelan equine encephalitis virus (VEEV) is an alphavirus that is endemic to the Americas. VEEV outbreaks occur periodically and cause encephalitis in both humans and equids. There are currently no therapeutics or vaccines for treatment of VEEV in humans. Our group has previously reported on the development of a benzamidine VEEV inhibitor, ML336, which shows potent antiviral activity in both in vitro and in vivo models of infection. In...
Source: Antiviral Therapy - Category: Virology Source Type: research
Authors: Craxì L Abstract In recent years, the debate on ethical issues related to hepatitis C virus therapies has been focused on the problem of drug prices and access to therapies. Nonetheless, the goal of hepatitis C virus eradication set by the World Health Organization in 2016 is raising new ethical issues, since governments are faced with a new challenge: reaching through screening, diagnosis and treatment a large amount of subjects with undiagnosed hepatitis C infection. National governments, especially high-income countries with a Welfare State, are compelled to provide access to therapies, but also ...
Source: Advances in Experimental Medicine and Biology - Category: Research Tags: Adv Exp Med Biol Source Type: research
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