Efficacy of purified nucleotide supplements on the growth performance and immunity of hybrid striped bass Morone chrysops x Morone saxatilis

Publication date: Available online 18 November 2019Source: Fish & Shellfish ImmunologyAuthor(s): Clement R. de Cruz, Fernando Y. Yamamoto, Min Ju, Kequan Chen, Alejandro Velasquez, Delbert M. GatlinAbstractFishmeal is being increasingly replaced in aquatic animal diets with alternative plant protein feedstuffs such as soybean meal which have lower concentrations of nucleotides; therefore, supplemental sources of exogenous nucleotides in diets could become increasingly important. A 9-week feeding trial was conducted with triplicate groups of juvenile hybrid striped bass (average initial body weight ± standard deviation, 5.6 ± 0.1 g) to determine the effects of supplementing single purified nucleotides on the growth performance and immune parameters. The basal diet, which utilized menhaden fishmeal (25%) and soybean meal (75%) as protein sources, contained 44% protein, 10% lipid and an estimated digestible energy level of 3.5 kcal g−1. Single additions of 5′- adenosine monophosphate (AMP), 5′- uridine monophosphate (UMP), 5′- cytidine monophosphate (CMP), 5′- guanosine monophosphate (GMP), and 5′- inosine monophosphate (IMP) disodium salts (Chem-Impex International, Wood Dale, Illinois, USA) were evaluated with each nucleotide added to the basal diet at 0.5% of dry weight at the expense of cellulose. A positive control diet in this trial was a diet containing 5′- AMP from Sigma-Aldrich also supplemented at 0.5% by weight. Results showed significant...
Source: Fish and Shellfish Immunology - Category: Biology Source Type: research