Application of Mass Spectrometry in Analysis of Non-Enzymatic Glycation Proteins in Diabetic Blood

Publication date: November 2019Source: Chinese Journal of Analytical Chemistry, Volume 47, Issue 11Author(s): Wei-Feng LI, De-Wen YAN, Yu JIN, Hai-Yan LI, Min MA, Zheng-Zhi WUAbstractDiabetes mellitus (DM) is a metabolic disorder disease characterized by hyperglycemia. Long-term physiological hyperglycemia will aggravate the degree of non-enzymatic glycation (NEG) proteins. Because the abnormal changes of non-enzymatic process can cause a series of pathological variation, NEG proteins have attracted more and more attention. Mass spectrometry (MS) is an indispensable and powerful analytical tool for the qualitative and quantitative analysis of proteins due to its ultra-high sensitivity, excellent detection limits, and multi-component simultaneous analysis, and has been applied to the identification and qualification of NEG proteins. Herein, the principle of NEG proteins and the application of MS in the identification and quantitation of NEG plasma proteins and hemoglobin (Hb) in blood are reviewed. The forecast in the analysis of NEG proteins based on MS is also provided.Graphical abstractNon-enzymatic glycation (NEG) are proteins of great significance for understanding the occurrence and development of diabetes mellitus and screening biomarkers. In this manuscript, the mechanism of NEG protein and application of mass spectrometry in the analysis of NEG proteins were reviewed. In addition, tendency of mass spectrometry for NEG proteins analysis in future was prospected.
Source: Chinese Journal of Analytical Chemistry - Category: Chemistry Source Type: research

Related Links:

Condition:   Bile Acid, Microbiota, Treatment of Diabetes Intervention:   Sponsor:   Chang Gung Memorial Hospital Not yet recruiting
Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials
Condition:   Type 2 Diabetes Mellitus Interventions:   Drug: Dapagliflozin Pill;   Drug: Empagliflozin Pill;   Drug: Placebo pill Sponsor:   Centro Universitario de Ciencias de la Salud, Mexico Not yet recruiting
Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials
CONCLUSIONS: This study, in line with previous studies, shows that a diagnosis of T1DM is associated with a decreased risk of schizophrenia. This finding of an inverse association between T1DM and schizophrenia may bring an interesting piece, related to autoimmunity, into the schizophrenia-aetiology puzzle. PMID: 31816222 [PubMed - as supplied by publisher]
Source: Neuroendocrinology Letters - Category: Endocrinology Tags: Neuro Endocrinol Lett Source Type: research
Abstract Human epididymis protein 4 (HE4) is an available tumor biomarker mainly for detecting ovarian cancer. However, it is unknown whether it can be a novel indicator for diagnosis of diabetic kidney disease (DKD). The aim of this study was to investigate the possibility of serum HE4 as a novel biomarker for DKD in patients with type 2 diabetes mellitus (T2DM). We enrolled 236 patients with T2DM and 82 healthy individuals. Serum HE4 was detected by ARCHITECT i2000 and compared between T2DM patients and healthy controls. The relationships between various variables and HE4 were analyzed by univariate or multivari...
Source: Biomed Res - Category: Research Authors: Tags: Biomed Res Int Source Type: research
This study is to identify the circular RNA (circRNA) expression profile that is functionally related to pancreatic islet β-cell autophagy and their potential regulation mechanisms in type 2 diabetes mellitus (T2DM). T2DM rat model was constructed by administration of high-fat and high-sugar diet. β-cells were isolated from islets by flow cytometry. CircRNA expression profile in β-cells was detected by circRNA microarrays, and the differentially expressed circRNAs were identified and validated by qRT-PCR. MicroRNA (miRNA) target prediction software and multiple bioinformatic approaches were used to construct ...
Source: Biomed Res - Category: Research Authors: Tags: Biomed Res Int Source Type: research
In this study, sitagliptin nanoparticles (sizes ranging from 210 to 618 nm) were developed. The bioadhesion properties of the nanoparticles, as well as the swelling of the nanoparticles on the mucus membrane aided in sustained drug release. The pattern of drug release was in accordance with the Peppas model. Fourier-transform infrared (FTIR) spectroscopy demonstrated that there were no significant interactions between sitagliptin and chitosan. Differential scanning calorimetry (DSC) results showed an absence of drug peaks due to the fact that the drug was present in an amorphous state. Mucoadhesive nanoparticles wer...
Source: Biomed Res - Category: Research Authors: Tags: Biomed Res Int Source Type: research
CONCLUSIONS: Reported complication rates associated with femoral VCD implantation are low. However, in some cases VCD implantation may account for severe complications including limb losses. Therefore, an adequate patient selection is necessary to detect those cases at higher risk for complications. PMID: 31814376 [PubMed - as supplied by publisher]
Source: International Angiology - Category: Cardiology Tags: Int Angiol Source Type: research
CONCLUSIONS: The presence of one or more prognostic factors as well as the various risk prediction models can guide selection of ACS patient subgroups for which a prophylactic CEA should or should not be offered. Decisions to offer prophylactic CEA should weigh in factors like surgeon's experience and hospital expertise/volume. Asymptomatic patients not expected to live long enough to benefit from the procedure should not be considered for CEA, but should be offered best medical treatment. PMID: 31814375 [PubMed - as supplied by publisher]
Source: International Angiology - Category: Cardiology Tags: Int Angiol Source Type: research
Publication date: Available online 5 November 2019Source: Nutrition, Metabolism and Cardiovascular DiseasesAuthor(s): Zeinab Naeini, Omid Toupchian, Akram Vatannejad, Gity Sotoudeh, Maryam Teimouri, Mostafa Ghorbani, Ensieh Nasli-Esfahani, Fariba KoohdaniAbstractBackground and AimsOmega-3 polyunsaturated fatty acids (PUFAs) are natural peroxisome proliferator-activated receptor gamma (PPAR-γ) ligands. Activated PPAR-γ protects the cardiovascular system against atherosclerotic lesion formation and exerts its anti-inflammatory role by suppressing cytokines induced by nuclear factor kappa-B (NF-κB) in endoth...
Source: Nutrition, Metabolism and Cardiovascular Diseases - Category: Nutrition Source Type: research
ConclusionsIn spite of the baseline negative preoperative UC, 2.9% of patients developed urosepsis after MPCNL. N+WBC+LE  + was determined to be an early and efficient prediction of intraoperative bacterial status and urosepsis following MPCNL. Nevertheless, further studies are needed to confirm the results.
Source: World Journal of Urology - Category: Urology & Nephrology Source Type: research
More News: Chemistry | China Health | Diabetes | Diabetes Mellitus | Endocrinology