Application of Mass Spectrometry in Analysis of Non-Enzymatic Glycation Proteins in Diabetic Blood

Publication date: November 2019Source: Chinese Journal of Analytical Chemistry, Volume 47, Issue 11Author(s): Wei-Feng LI, De-Wen YAN, Yu JIN, Hai-Yan LI, Min MA, Zheng-Zhi WUAbstractDiabetes mellitus (DM) is a metabolic disorder disease characterized by hyperglycemia. Long-term physiological hyperglycemia will aggravate the degree of non-enzymatic glycation (NEG) proteins. Because the abnormal changes of non-enzymatic process can cause a series of pathological variation, NEG proteins have attracted more and more attention. Mass spectrometry (MS) is an indispensable and powerful analytical tool for the qualitative and quantitative analysis of proteins due to its ultra-high sensitivity, excellent detection limits, and multi-component simultaneous analysis, and has been applied to the identification and qualification of NEG proteins. Herein, the principle of NEG proteins and the application of MS in the identification and quantitation of NEG plasma proteins and hemoglobin (Hb) in blood are reviewed. The forecast in the analysis of NEG proteins based on MS is also provided.Graphical abstractNon-enzymatic glycation (NEG) are proteins of great significance for understanding the occurrence and development of diabetes mellitus and screening biomarkers. In this manuscript, the mechanism of NEG protein and application of mass spectrometry in the analysis of NEG proteins were reviewed. In addition, tendency of mass spectrometry for NEG proteins analysis in future was prospected.
Source: Chinese Journal of Analytical Chemistry - Category: Chemistry Source Type: research