Transcriptomic Profile and Sexual Reproduction-Relevant Genes of Alexandrium minutum in Response to Nutritional Deficiency

Alexandrium minutum is a typical marine toxic dinoflagellate responsible for producing paralytic shellfish poisoning (PSP) toxins. Until now, we know little about the genomic information of A. minutum, so a transcriptome study was conducted to clarify the physiological adaptations related to nutritional deficiency. Here, we performed RNA-Seq analysis to assess the gene expression patterns of A. minutum under N and P deficient conditions for 0 (control), 6, and 72 h. Main differences between the control and experimental groups were observed in hydrolase activity and fatty acid, lipid, protein, and P metabolism. Activities of photosystem I (PSI) and PSII were significantly down-regulated, and the endocytosis pathway (clathrin-dependent endocytosis) was significantly enriched under N and P stress compared with the control, indicating that A. minutum shifts its trophy pattern under N and P stress. We also identified several unigenes related to the process of sexual reproduction, including sex determination, sperm-egg recognition, sex differentiation, mating, and fertilization. Approximately 50% of the successfully annotated unigenes were differentially expressed between the short-term stimulated sample (6 h) and control (R). However, the expression level of most unigenes returned to normal levels after 72 h, indicating that N and P stress plays a limited role in the induction of sexual reproduction. Furthermore, the quantitative real-time PCR (qRT-PCR) results of the five represe...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research

Related Links:

Suicide is a major public health concern in South Korea, and self-poisoning by pesticides is one of the common methods of suicide. Pesticide ban policies have been successful for suicide prevention; however, no studies have shown their effect according to ...
Source: SafetyLit - Category: International Medicine & Public Health Tags: Home and Consumer Product Safety Source Type: news
Introduction Emergency department observation units (EDOU) have been shown to be effective in decreasing hospitalization rates and length of stay (LOS) for various conditions. However, cost savings and efficacy in the management of poisoning in EDOU have n...
Source: SafetyLit - Category: International Medicine & Public Health Tags: Economics of Injury and Safety, PTSD, Injury Outcomes Source Type: news
Authors: Montrucchio G, Bosso S, Scanu M, Mina A, Imeneo MR, Brazzi L PMID: 31818086 [PubMed - as supplied by publisher]
Source: Minerva Anestesiologica - Category: Anesthesiology Tags: Minerva Anestesiol Source Type: research
(Duke University) Scientists from Duke University have developed a model that can predict the amount of mercury being released into a local ecosystem from deforestation. The research could point toward ways to mitigate the worst effects of mercury poisoning in regions already experiencing elevated mercury levels caused by small-scale gold mining practices, such as those in the Peruvian Amazon.
Source: EurekAlert! - Medicine and Health - Category: International Medicine & Public Health Source Type: news
Foods may potentially serve as vehicles for the transmission of antimicrobial-resistant variants of Staphylococcus aureus that are important in a human clinical context. Further, retail food products can be a cause of staphylococcal food poisoning. For these reasons and to account for source attribution and risk assessment, detailed information on the population structure, resistance, and virulence profiles of S. aureus originating from retail food products is necessary. In the current study, whole-genome sequences from 88 S. aureus isolates were subjected to bioinformatics analyses in relation to sequence types, antimicro...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Publication date: Available online 10 December 2019Source: Journal of Electroanalytical ChemistryAuthor(s): Shichao Zhu, Aijuan Xie, Xiang Tao, Jianghui Zhang, Bingyan Wei, Zhongxiang Liu, Yuwei Tao, Shiping LuoAbstractThe novel CoCu metal-organic framework (CoCu-MOF) precursor and CoCu-MOF/polyaniline (CoCu-MOF/PANI) electrode materials were synthesized by hydrothermal method and in situ polymerization, respectively. The as-synthesized CoCu-MOF/PANI and corresponding composite were first tested for their electrocatalytic performance towards glycerol oxidation in KOH solution and then the selection and optimization were fu...
Source: Journal of Electroanalytical Chemistry - Category: Chemistry Source Type: research
In this study, we aimed to evaluate the effects of different C/N substrates on RSD and to explore the changes in microbial community structure during RSD treatment. The experimental set up included 10 groups, as follows: CK, without substrates; RSD treatments with alfalfa (Medicago sativa L.)[AL], maize (Zea mays Linn. Sp.) straw [MS], and rice (Oryza sativa L.) straw [RS], with three levels of addition (0.5% [L], 2% [M], and 5% [H]), yielding ALL, ALM, ALH, MSL, MSM, MSH, RSL, RSM, and RSH groups. Compared with CK, RSD treatments significantly increased the content of NH4+-N, and effectively eliminated the accumulated NO3...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
The prevalence of deadly opioids in western Canadian prisons has inmates and the officers who guard them increasingly fearful the drugs may be weaponized, a new University of Alberta study suggests.
Source: CBC | Health - Category: Consumer Health News Tags: News/Canada/Edmonton Source Type: news
Condition:   Toxicity;Chemical Intervention:   Sponsor:   Assiut University Active, not recruiting
Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials
J. Mater. Chem. A, 2020, Advance Article DOI: 10.1039/C9TA11745H, CommunicationHongdong Li, Yue Pan, Dan Zhang, Yi Han, Zuochao Wang, Yingnan Qin, Shuangyan Lin, Xueke Wu, Huan Zhao, Jianping Lai, Bolong Huang, Lei Wang The surface-oxygen-modified ultrathin Pt62Ru18Ni20–O/C nanowires catalyst exhibits outstanding mass activity, excellent stability, and CO anti-poisoning for methanol oxidation reaction in acidic medium by DFT and electrochemical experiments. To cite this article before page numbers are assigned, use the DOI form of citation above. The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - J. Mater. Chem. latest articles - Category: Chemistry Authors: Source Type: research
More News: Genetics | Microbiology | Nutrition | Poisoning | Reproduction Medicine | Study | Toxicology