Overexpression of Chimeric RNA by Retroviral Transduction.

Overexpression of Chimeric RNA by Retroviral Transduction.
Methods Mol Biol. 2020;2079:155-166
Authors: Wu H, Li H
Abstract
Cloning the open reading frame (ORF) of a protein-coding chimeric RNA into a mammalian expression vector is a simple, practiced way to study the function of the chimeric RNA. Here, we provide procedures for the insertion of the RRM2-C2orf48 fusion ORF into a mammalian expression vector, achieving the overexpression of the RRM2-C2orf48 fusion protein in a colon cancer cell line by retroviral transduction.
PMID: 31728969 [PubMed - in process]

Source: Mol Biol Cell - November 19, 2019 Category: Molecular Biology Authors: Wu H, Li H Tags: Methods Mol Biol Source Type: research