Isolation of Neutrophils from Larval Zebrafish and Their Transplantation into Recipient Larvae for Functional Studies.

Isolation of Neutrophils from Larval Zebrafish and Their Transplantation into Recipient Larvae for Functional Studies. Methods Mol Biol. 2020;2087:61-75 Authors: Darroch H, Astin JW, Hall CJ Abstract Live imaging of neutrophils within optically transparent larval zebrafish has proved a powerful technique to investigate how specific gene products control neutrophil function. To resolve whether a gene contributes to neutrophil function in a cell-autonomous manner necessitates a way to examine gene-deficient neutrophils in an otherwise wild type background. To this end, here we describe methods to harvest fluorescent neutrophils from larval donor zebrafish and transplant them into age-matched recipients. We show that transplanted neutrophils can survive in recipient larvae for at least 3 days providing ample opportunity for functional studies. Focusing on bactericidal activity, we show that transplanted neutrophils phagocytose and kill live bacteria with similar kinetics to nontransplanted neutrophils, indicating that the transplantation process does not influence these neutrophil effector functions. Following the methods described here to transplant neutrophils between gene-deficient and wild type larval zebrafish will enable investigations into whether a gene's contribution to neutrophil function is cell-autonomous. PMID: 31728983 [PubMed - in process]
Source: Mol Biol Cell - Category: Molecular Biology Authors: Tags: Methods Mol Biol Source Type: research

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Publication date: Available online 9 December 2019Source: Canadian Journal of CardiologyAuthor(s): Eli Muchtar, Grace Lin, Martha GroganAbstractLight chain (AL) amyloidosis is a systemic syndrome characterized by progressive organ dysfunction leading to organ failure and death. The heart is the most commonly involved organ and the leading determinant of short and long-term survival. Pathogenic free light chains, fragments of intact immunoglobulins, are the amyloidogenic protein and are secreted by clonal bone marrow plasma cells. The goal of therapy is to cut-off the supply of these pathogenic light chains to allow organ r...
Source: Canadian Journal of Cardiology - Category: Cardiology Source Type: research
Authors: Montgomery E, Macdonald PS, Jha SR, Newton PJ, Malouf M PMID: 31815560 [PubMed - as supplied by publisher]
Source: Expert Review of Respiratory Medicine - Category: Respiratory Medicine Tags: Expert Rev Respir Med Source Type: research
ConclusionThere is a high prevalence of cognitive impairment identified by neuropsychological tests in older patients with hematological malignancies evaluated for HCT. Identification of an appropriate MoCA cut-off score in this population is important to identify patients who would benefit from further assessment.
Source: Journal of Geriatric Oncology - Category: Cancer & Oncology Source Type: research
Authors: Ye X, Zhou XJ, Zhang H Abstract Autophagy is an important biology process, central to the maintenance of biology process in both physiological and pathological situations. It is regarded as a "double-edged sword"-exerting both protective and/or detrimental effects. These two-way effects are observed in immune cells as well as renal resident cells, including podocytes, mesangial cells, tubular epithelial cells, and endothelial cells of the glomerular capillaries. Mounting evidence suggests that autophagy is implicated in the pathological process of various immune-related renal diseases (IRRDs) as ...
Source: Journal of Immunology Research - Category: Allergy & Immunology Tags: J Immunol Res Source Type: research
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Source: Restorative Neurology and Neuroscience - Category: Neurology Tags: Restor Neurol Neurosci Source Type: research
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Source: Student Doctor Network - Category: Universities & Medical Training Authors: Tags: Cardiology Source Type: forums
Researchers from Case Western Reserve University in Ohio found those who lived in areas with high pollution levels were 26 per cent more likely to die within 4.8 years of the operation.
Source: the Mail online | Health - Category: Consumer Health News Source Type: news
In only the third documented such procedure, an international team of surgeons met in Belgrade, Serbia, to transplant a testicle from one twin to the other, restoring testosterone.
Source: the Mail online | Health - Category: Consumer Health News Source Type: news
(NIH/National Cancer Institute) New findings from a clinical trial show that treatment with the immunotherapy drug blinatumomab is superior to standard chemotherapy for children and young adults with high- or intermediate-risk B-cell acute lymphoblastic leukemia (B-ALL) that has relapsed. Those treated with blinatumomab had longer survival, experienced fewer severe side effects, had a higher rate of undetectable residual disease, and were more likely to proceed to a stem cell transplant.
Source: EurekAlert! - Cancer - Category: Cancer & Oncology Source Type: news
Journal Name: Clinical Chemistry and Laboratory Medicine (CCLM) Issue: Ahead of print
Source: Clinical Chemistry and Laboratory Medicine - Category: Laboratory Medicine Source Type: research
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