Isolation of Neutrophils from Larval Zebrafish and Their Transplantation into Recipient Larvae for Functional Studies.
Isolation of Neutrophils from Larval Zebrafish and Their Transplantation into Recipient Larvae for Functional Studies. Methods Mol Biol. 2020;2087:61-75 Authors: Darroch H, Astin JW, Hall CJ Abstract Live imaging of neutrophils within optically transparent larval zebrafish has proved a powerful technique to investigate how specific gene products control neutrophil function. To resolve whether a gene contributes to neutrophil function in a cell-autonomous manner necessitates a way to examine gene-deficient neutrophils in an otherwise wild type background. To this end, here we describe methods to harvest fluorescent neutrophils from larval donor zebrafish and transplant them into age-matched recipients. We show that transplanted neutrophils can survive in recipient larvae for at least 3 days providing ample opportunity for functional studies. Focusing on bactericidal activity, we show that transplanted neutrophils phagocytose and kill live bacteria with similar kinetics to nontransplanted neutrophils, indicating that the transplantation process does not influence these neutrophil effector functions. Following the methods described here to transplant neutrophils between gene-deficient and wild type larval zebrafish will enable investigations into whether a gene's contribution to neutrophil function is cell-autonomous. PMID: 31728983 [PubMed - in process]
Publication date: Available online 9 December 2019Source: Canadian Journal of CardiologyAuthor(s): Eli Muchtar, Grace Lin, Martha GroganAbstractLight chain (AL) amyloidosis is a systemic syndrome characterized by progressive organ dysfunction leading to organ failure and death. The heart is the most commonly involved organ and the leading determinant of short and long-term survival. Pathogenic free light chains, fragments of intact immunoglobulins, are the amyloidogenic protein and are secreted by clonal bone marrow plasma cells. The goal of therapy is to cut-off the supply of these pathogenic light chains to allow organ r...
Authors: Montgomery E, Macdonald PS, Jha SR, Newton PJ, Malouf M PMID: 31815560 [PubMed - as supplied by publisher]
ConclusionThere is a high prevalence of cognitive impairment identified by neuropsychological tests in older patients with hematological malignancies evaluated for HCT. Identification of an appropriate MoCA cut-off score in this population is important to identify patients who would benefit from further assessment.
Authors: Ye X, Zhou XJ, Zhang H Abstract Autophagy is an important biology process, central to the maintenance of biology process in both physiological and pathological situations. It is regarded as a "double-edged sword"-exerting both protective and/or detrimental effects. These two-way effects are observed in immune cells as well as renal resident cells, including podocytes, mesangial cells, tubular epithelial cells, and endothelial cells of the glomerular capillaries. Mounting evidence suggests that autophagy is implicated in the pathological process of various immune-related renal diseases (IRRDs) as ...
Authors: Zhang X, Tenerelli K, Wu S, Xia X, Yokota S, Sun C, Galvao J, Venugopalan P, Li C, Madaan A, Goldberg JL, Chang KC Abstract BACKGROUND: Glaucoma, the number one cause of irreversible blindness, is characterized by the loss of retinal ganglion cells (RGCs), which do not regenerate in humans or mammals after cell death. Cell transplantation provides an opportunity to restore vision in glaucoma, or other optic neuropathies. Since transplanting primary RGCs from deceased donor tissues may not be feasible, stem cell-derived RGCs could provide a plausible alternative source of donor cells for transplant. OBJ...
I used this forum a lot in the past. Here is my 2 cents and time to give back. The data listed below represent the info for the calendar year of 2019, and can be different if you look up next year or later. First off, it's difficult to understand &compare what each institution does as essentially no two program will train you the same way. However, certain things are quantifiable such as PCI volume, transplant, ICDs etc. Please find some helpful links to databases below: Transplant: [URL... Cardiology fellowship program reviews. Facts and impressions
Researchers from Case Western Reserve University in Ohio found those who lived in areas with high pollution levels were 26 per cent more likely to die within 4.8 years of the operation.
In only the third documented such procedure, an international team of surgeons met in Belgrade, Serbia, to transplant a testicle from one twin to the other, restoring testosterone.
(NIH/National Cancer Institute) New findings from a clinical trial show that treatment with the immunotherapy drug blinatumomab is superior to standard chemotherapy for children and young adults with high- or intermediate-risk B-cell acute lymphoblastic leukemia (B-ALL) that has relapsed. Those treated with blinatumomab had longer survival, experienced fewer severe side effects, had a higher rate of undetectable residual disease, and were more likely to proceed to a stem cell transplant.
Journal Name: Clinical Chemistry and Laboratory Medicine (CCLM) Issue: Ahead of print