Analysis of Neutrophil Transmigration Through Epithelial Cell Monolayers.

Analysis of Neutrophil Transmigration Through Epithelial Cell Monolayers. Methods Mol Biol. 2020;2087:79-91 Authors: Kirpotina LN, Kominsky DJ, Quinn MT, Swain SD Abstract Transmigration of neutrophils through an epithelial layer, such as in the intestine or lung, is a necessary response to a perceived attack at the mucosal surface of that tissue. This process is dynamically regulated by a number of interactive events between the neutrophil and other cell types and allows for an effective and localized neutrophil response. However, in certain inflammatory diseases, including inflammatory bowel disease and chronic obstructive pulmonary disease (COPD), persistent neutrophil accumulation can contribute to disease pathology. Elucidating the mechanisms of this aberrant neutrophil accumulation is crucial for understanding and ameliorating these disease processes. The method we describe here is a controlled model system that allows for the investigation of the interactive signals involved in neutrophil transmigration through epithelial barriers, and possible mechanisms of deregulation of this process. PMID: 31728984 [PubMed - in process]
Source: Mol Biol Cell - Category: Molecular Biology Authors: Tags: Methods Mol Biol Source Type: research

Related Links:

Conclusion: concomitant intestinal pathology is detected in every third patient with COPD, with a predominance of functional disorders. There is a mutual aggravation of the symptoms of the disease, especially in the presence of complications.
Source: European Respiratory Journal - Category: Respiratory Medicine Authors: Tags: Monitoring airway disease Source Type: research
Abstract BACKGROUND: There is a growing evidence regarding an increased risk of inflammatory bowel disease (IBD) among patients with airway diseases. AIM: To investigate the influence of chronic obstructive pulmonary disease (COPD) on the risk of IBD. METHODS: A nationwide, population-based study was conducted using data from the National Health Insurance Service database. A total of 1303021 patients with COPD and 6515105 non-COPD controls were identified. The COPD group was divided into the severe and the mild COPD group according to diagnostic criteria. The risk of IBD in patients with COPD compared to...
Source: World Journal of Gastroenterology : WJG - Category: Gastroenterology Authors: Tags: World J Gastroenterol Source Type: research
There is evidence of an association between inflammatory bowel disease (IBD) and lung conditions such as chronic obstructive pulmonary disease (COPD). This systematic review and meta-analysis explored the risk...
Source: BMC Pulmonary Medicine - Category: Respiratory Medicine Authors: Tags: Research article Source Type: research
te; G Abstract Immune-mediated inflammatory diseases (IMIDs) encompass a wide range of seemingly unrelated conditions, such as multiple sclerosis, rheumatoid arthritis, psoriasis, inflammatory bowel diseases, asthma, chronic obstructive pulmonary disease, and systemic lupus erythematosus. Despite differing etiologies, these diseases share common inflammatory pathways, which lead to damage in primary target organs and frequently to a plethora of systemic effects as well. The purinergic signaling complex comprising extracellular nucleotides and nucleosides and their receptors, the P2 and P1 purinergic receptors, res...
Source: Pharmacological Reviews - Category: Drugs & Pharmacology Authors: Tags: Pharmacol Rev Source Type: research
Poshmaal Dhar1 and Julie McAuley2* 1Faculty of Health, School of Medicine, Deakin University, Geelong, VIC, Australia 2Department of Microbiology and Immunology, Peter Doherty Institute, University of Melbourne, Melbourne, VIC, Australia The family of cell surface (cs-) mucins are constitutively expressed at the cell surface by nearly all epithelial cells, beneath the gel-mucin layer. All cs-mucin family members have structural features that enable them to act as a releasable decoy barrier to mucosal pathogens, by providing ligands for pathogen binding and the ability to shed the bound extracellular domain. Due ...
Source: Frontiers in cellular and infection microbiology - Category: Microbiology Source Type: research
CD4+ T-Cells With High Common γ Chain Expression and Disturbed Cytokine Production Are Enriched in Children With Type-1 Diabetes Julia Seyfarth1*, Nathalie Mütze1, Jennifer Antony Cruz1, Sebastian Kummer1, Christina Reinauer1, Ertan Mayatepek1, Thomas Meissner1,2 and Marc Jacobsen1 1Department of General Pediatrics, Neonatology and Pediatric Cardiology, University Children's Hospital, Medical Faculty, Düsseldorf, Germany 2German Center for Diabetes Research (DZD), Partner Düsseldorf, Düsseldorf, Germany The common gamma chain (γc) contributes to the formation of different c...
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research
Authors: Ribaldone DG, Pellicano R, Actis GC Abstract An increasing deal of attention is being conveyed on the extra-intestinal manifestations of inflammatory bowel diseases (IBD). We compiled the present review in an attempt to upgrade the accuracy of the classification of such polymorphic entities. We focused on three patterns. First, the conventional extra-intestinal manifestations localized to bone and joints, to the eye, to the biliary tree and to the skin. Second, the so-called IBD-like syndromes accompanied by bone marrow-derived anomalies of innate or acquired immunity. Third, specific disorders of the skin...
Source: Minerva Gastroenterologica e Dietologica - Category: Gastroenterology Tags: Minerva Gastroenterol Dietol Source Type: research
In this study they also showed PTX3 localized in NETs formed after neutrophil activation (5). Proteomics analysis revealed that PTX3 forms complexes with two anti-microbial proteins [azurocidin (AZU1) and myeloperoxidase (MPO)] associated to NETs (30). More recently, PTX3 localization in NETs has been confirmed, and the colocalization with AZU1 and MPO has been defined more accurately (31). Further investigation will be needed to understand the involvement of PTX3 interaction with AZU1 and MPO in their antibacterial role during NET formation. Regulation of Complement Activation PTX3 interaction with microorganisms is not...
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research
Discussion There are numerous microbial communities inhabited in the human body, which is critical to human health. The relationship between human microbiome and diseases received much attention from both medical and bioinformatics community recently. However, traditional methods to detect their association is costly and labor-intensive. Thus, we proposed here a new computational model called NBLPIHMDA to infer potential microbe-disease associations. NBLPIHMDA first combined known microbe-disease associations in HMDAD and the Gaussian interaction profile kernel similarity to construct disease similarity network and microb...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Authors: Serveaux-Dancer M, Jabaudon M, Creveaux I, Belville C, Blondonnet R, Gross C, Constantin JM, Blanchon L, Sapin V Abstract The receptor for advanced glycation end-products (RAGE) is a cell surface transmembrane multiligand receptor, encoded by the AGER gene. RAGE presents many transcripts, is expressed mainly in the lung, and involves multiple pathways (such as NFκB, Akt, p38, and MAP kinases) that initiate and perpetuate an unfavorable proinflammatory state. Due to these numerous functional activities, RAGE is implicated in multiple diseases. AGER is a highly polymorphic gene, with polymorphisms or S...
Source: Disease Markers - Category: Laboratory Medicine Tags: Dis Markers Source Type: research
More News: Chronic Obstructive Pulmonary | Inflammatory Bowel Disease | Molecular Biology | Pathology