Down-regulated lncRNA HOTAIR alleviates polycystic ovaries syndrome in rats by reducing expression of insulin-like growth factor 1 via microRNA-130a.

Down-regulated lncRNA HOTAIR alleviates polycystic ovaries syndrome in rats by reducing expression of insulin-like growth factor 1 via microRNA-130a. J Cell Mol Med. 2019 Nov 16;: Authors: Jiang B, Xue M, Xu D, Song J, Zhu S Abstract It has been found that long noncoding RNA HOTAIR, microRNA-130a (miR-130a) and insulin-like growth factor 1 (IGF1) expression are associated with ovarian cancer, thus, we hypothesised that the HOTAIR/miR-130a/IGF1 axis might associate with endocrine disorders and biological behaviours of ovarian granulosa cells in rat models of polycystic ovary syndrome (PCOS). PCOS rat models were established by injection of dehydro-isoandrosterone, followed by treatment of si-HOTAIR, oe-HOTAIR, miR-130a mimics or miR-130a inhibitors. Serum hormonal levels were determined to evaluate endocrine conditions. The effect of HOTAIR and miR-130a on activities of isolated ovarian granulosa cells was assessed, as well as the involvement of IGF1.In the ovarian tissues and granulosa cells of PCOS rat models, highly expressed HOTAIR and IGF1 and poorly expressed miR-130a were identified. In response to oe-HOTAIR, serum levels of E2 , T and LH were increased and serum levels of FSH were reduced; the proliferation of granulosa cells was reduced and apoptosis was promoted; notably, expression of miR-130a was reduced while expression of IGF1 was increased. The treatment of si-HOTAIR reversed the situation. Furthermore, the binding of HOTAIR to miR-130a and...
Source: J Cell Mol Med - Category: Molecular Biology Authors: Tags: J Cell Mol Med Source Type: research

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AbstractWhile childbearing protects against risk of epithelial ovarian cancer (EOC), few studies have explored the impact on maternal EOC risk of sex of offspring, which may affect the maternal environment during pregnancy. We performed a pooled analysis among parous participants from 12 case –controls studies comprising 6872 EOC patients and 9101 controls. Odds ratios (ORs) and 95% confidence intervals (CIs) were calculated using multivariable logistic regression for case–control associations and polytomous logistic regression for histotype-specific associations, all adjusted for po tential confounders. In gen...
Source: European Journal of Epidemiology - Category: Epidemiology Source Type: research
AbstractSTUDY QUESTIONDo young women with early ovarian ageing (EOA), defined as unexplained, and repeatedly few oocytes harvested in ART have an increased risk of age-related events?SUMMARY ANSWERAt follow-up, women with idiopathic EOA had an increased risk of age-related events compared to women with normal ovarian ageing (NOA).WHAT IS KNOWN ALREADYEarly and premature menopause is associated with an increased risk of cardiovascular diseases (CVDs), osteoporosis and death. In young women, repeated harvest of few oocytes in well-stimulated ART cycles is a likely predictor of advanced menopausal age and may thus serve as an...
Source: Human Reproduction - Category: Reproduction Medicine Source Type: research
Abstract Ephrins are ligands of Eph receptors (Ephs); both of which are sorted into two classes, A and B. There are five types of ephrin-As (ephrin-A1-5) and three types of ephrin-Bs (ephrin-B1-3). Also, there are ten types of EphAs (EphA1-10) and six types of EphBs (EphB1-6). Binding of ephrins to the Eph receptors activates signaling cascades that regulate several biological processes such as cellular proliferation, differentiation, migration, angiogenesis and vascular remodeling. Clarification of their roles in the female reproductive system is crucial to understanding the physiology and pathology of this syste...
Source: Biology of Reproduction - Category: Reproduction Medicine Authors: Tags: Biol Reprod Source Type: research
AbstractAnti-M üllerian Hormone (AMH) is critical for physiologic involution of the Mullerian ducts during sexual differentiation in the male fetus. In women, AMH is a product of the small antral follicles in the ovaries and serves to function as an autocrine and paracrine regulator of follicular maturation. As t he size of the residual follicular pool depends on the quantity of small antral follicles and declines over time, the serum AMH level in women follows a characteristic trajectory: a gradual decline throughout the reproductive years and a precipitous drop at menopause, becoming undetectable soon afte r. Thus, ...
Source: The Journal of Obstetrics and Gynecology of India - Category: OBGYN Source Type: research
ConclusionsThis study confirms recent investigations demonstrating that conventional HKGs, such as GAPDH and beta-actin, are not suitable reference genes for specific pathological conditions, emphasizing the importance of determining the best HKGs on a case-by-case basis and according to tissue type. Our results have identified reliable HKGs for studies of normal human ovaries and those affected by OEA, OMA, OSPC, or PCOS, as well as combined studies of control subjects vs. each cancer or PCOS group.
Source: Journal of Assisted Reproduction and Genetics - Category: Reproduction Medicine Source Type: research
Conclusion: There was no difference in the ovarian protective effect according to the difference in the timing of administration of GnRHa. PMID: 32595989 [PubMed]
Source: Journal of Breast Cancer - Category: Cancer & Oncology Tags: J Breast Cancer Source Type: research
The objective of this review is to summarize our knowledge about the contribution of vitamin D3 to physiological and pathological processes within the ovary. PMID: 32469224 [PubMed - as supplied by publisher]
Source: Physiological Research - Category: Physiology Authors: Tags: Physiol Res Source Type: research
Polycystic ovary syndrome (PCOS) is a metabolic and hormonal disorder characterized by hyperandrogenism, oligoanovulation, and polycystic ovarian morphology. Women with PCOS are at a higher risk for health issues, ranging from endocrine conditions and reproductive comorbidities to cardiovascular complications. Regarding its effect on breast cancer, which is the most common female cancer, androgen excess has been postulated to play a part in carcinogenesis, especially in estrogen receptor (ER) –positive breast cancer.
Source: American Journal of Obstetrics and Gynecology - Category: OBGYN Authors: Tags: Research Letter Source Type: research
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Source: Molecular and Cellular Endocrinology - Category: Endocrinology Source Type: research
Conclusions: Environmental toxin exposure in PCOS women could favour neoplastic transformation by exacerbating and potentiating some PCOS features. Further research, although difficult, is needed in order to prevent further diffusion of these substances in the environment, or at least to provide adequate information to the population considered at risk. PMID: 32046531 [PubMed - as supplied by publisher]
Source: Current Medical Research and Opinion - Category: Research Tags: Curr Med Res Opin Source Type: research
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