The inhibition of lactoperoxidase catalytic activity through mesna (2-mercaptoethane sodium sulfonate).
The inhibition of lactoperoxidase catalytic activity through mesna (2-mercaptoethane sodium sulfonate). J Inorg Biochem. 2019 Nov 12;203:110911 Authors: Jahanbakhsh S, Dekhne MS, Kohan-Ghadr HR, Bai D, Awonuga A, Morris RT, Yang Z, Abu-Soud HM Abstract Here, we show that mesna (sodium-2-mercaptoethane sulfonate), primarily used to prevent nephrotoxicity and urinary tract toxicity caused by chemotherapeutic agents such as cyclophosphamide and ifosfamide, modulates the catalytic activity of lactoperoxidase (LPO) by binding tightly to the enzyme, functioning either as a one electron substrate for LPO Compounds I and II, destabilizing Compound III. Lactoperoxidase is a hemoprotein that utilizes hydrogen peroxide (H2O2) and thiocyanate (SCN-) to produce hypothiocyanous acid (HOSCN), an antimicrobial agent also thought to be associated with carcinogenesis. Our results revealed that mesna binds stably to LPO within the SCN- binding site, dependent of the heme iron moiety, and its combination with LPO-Fe(III) is associated with a disturbance in the water molecule network in the heme cavity. At low concentrations, mesna accelerated the formation and decay of LPO compound II via its ability to serve as a one electron substrate for LPO compounds I and II. At higher concentrations, mesna also accelerated the formation of Compound II but it decays to LPO-Fe(III) directly or through the formation of an intermediate, Compound I*, that displays characteristic spectrum s...
Authors: Rohanachandra YM, Amarabandu HGI, Rohanachandra LT Abstract Parenting with mental illness is associated with family conflicts, parenting difficulties, low parental confidence and increased mental health and behavioural problems in children. Family focused interventions improve child outcomes by about 40 %. However, such services are not available in Sri Lanka.A cross sectional descriptive study was carried out in the general adult psychiatry follow-up clinics in a Teaching Hospital in Colombo, Sri Lanka to assess the needs of parents with mental illness. A specifically designed interviewer administered que...
Authors: Charnsil C, Narkpongphun A, Chailangkarn K Abstract AIMS: This research examines the prevalence of post-traumatic stress disorder (PTSD) and its related factors in students whose school burned down, one month and six months after the incident. METHODS: A total of 56 students from grades one through six were invited to participate in this study with permission from their parents. Subjects were screened for PTSD by using UCLA PTSD Reaction Index (DSM-5 Version) during three periods: 1, 6, and 12 months after the incident. Children diagnosed with PTSD were interviewed by a child and adolescent psychiatris...
Consultations in Liver Disease
Vinod K. Rustgi
NORMAN GITLIN, MD, FRCP (LONDON), FRCPE (EDINBURGH), FAASLD, FACP, FACG
CLINICS IN LIVER DISEASE
Hippocrates (460-371 bc) first recognized the relationship between the neuropsychological and physiologic changes observed in liver disease (Figs. 1 and 2). At that time, Hippocrates noted that there was a poor clinical outcome associated with delirium that was preceded by jaundice.1
We present a brief overview of the potential prophylactic and treatment agents under investigation, some which could be initiated in the ED if proven effective.
[Daily Maverick] While the promise of repositioning 'old' drugs such as chloroquine and hydroxychloroquine is tantalising, it is critical that the hazards of using unapproved treatments are shared with the public to prevent needless fatalities and supply shortages that may further burden already strained healthcare systems.