Rapid differentiation of Campylobacter jejuni cell wall mutants using Raman spectroscopy, SERS and mass spectrometry combined with chemometrics
Analyst, 2019, Accepted Manuscript DOI: 10.1039/C9AN02026H, PaperMalama Chisanga, Dennis Linton, Howbeer Muhamadali, David I. Ellis, Richard Kimber, Aleksandr Mironov, Royston Goodacre The Gram-negative bacterial pathogen Campylobacter jejuni is a major cause of foodborne gastroenteritis worldwide. Rapid detection and identification of C. jejuni informs timely prescription of appropriate therapeutics and epidemiological investigations.... The content of this RSS Feed (c) The Royal Society of Chemistry
In this study, we evaluated the efficacy of our two previously developed real time-quantitative PCR (qPCR) assays for the detection of swine-associated Bacteroidales genetic markers (gene 1–38, gene 3–53) in the Yangtze Delta watershed of southeastern China. The results indicated that the gene 1–38 and 3-53 markers exhibited high accuracy (92.5%, 91.7% conditional probability, respectively) in detecting Bacteroidales spp. in water samples. According to binary logistic regression (BLR), these two swine-associated markers were well correlated (P
Publication date: Available online 5 December 2019Source: Research in Veterinary ScienceAuthor(s): Marta Perez-Sancho, Teresa García-Seco, Concepción Porrero, Nerea García, Susana Gomez-Barrero, Jose María Cámara, Lucas Domínguez, Julio ÁlvarezAbstractFeral pigeons have increased in urban settings worldwide becoming a potential health risk for humans and other animals. Control and surveillance programs are essential to prevent the possible transmission of zoonotic pathogens carried by pigeons. A surveillance program was carried out in Madrid City (Spain) during 2005–20...
Microbial Drug Resistance, Ahead of Print.
Authors: Guirin GF, Brusa V, Adriani CD, Leotta GA Abstract We studied and compared the prevalence of Campylobacter jejuni and Campylobacter coli in chicken carcasses from conventional and kosher broiler abattoirs and retail stores. The prevalence of thermotolerant Campylobacter-positive carcasses was 94.0 (kosher) and 32.0% (conventional) (p
Condition: Campylobacter Infections Intervention: Biological: LMN-101 Sponsors: Lumen Bioscience, Inc.; Naval Medical Research Center; Pharmaron CPC, Inc.; University of Maryland, College Park Not yet recruiting
Contributors : Praveen K Nattramilarasu ; Roland B ücker ; Fábia D Lobo de Sá ; Anja Fromm ; Oliver Nagel ; In-Fah M Lee ; Eduard Butkevych ; Markus M Heimesaat ; Stefan Bereswill ; Michal R Schweiger ; Hans L Nielsen ; Hanno Troeger ; Jörg-Dieter SchulzkeSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensThe study aimed to characterize C. concisus effects on ENaC using the HT-29/B6-GR/MR cell model.
This study aimed to assess the efficacy of four different sanitizers in reducing the microbial load from naturally contaminated chicken meat carcasses collected from the processing plants in South Australia. There was a significant variation in a load of Campylobacter and total viable count (TVC) between samples collected from two different processing plants and within carcass batches collected from the same plant that was tested during the study. All sanitizers generally reduced the load of Campylobacter on chicken meat carcasses. Treatment with acidified sodium chlorite significantly reduced the level of Salmonella enter...
Analyst, 2020, Advance Article DOI: 10.1039/C9AN02026H, PaperMalama Chisanga, Dennis Linton, Howbeer Muhamadali, David I. Ellis, Richard L. Kimber, Aleksandr Mironov, Royston Goodacre SERS was developed for intercellular and intracellular analyses. Using a series of cell wall mutants inC. jejuni we show cell wallversus cytoplasm differences. To cite this article before page numbers are assigned, use the DOI form of citation above. The content of this RSS Feed (c) The Royal Society of Chemistry
(NEW YORK) — Go ahead and rinse your cranberries, potatoes and green beans. But food experts say don’t — repeat don’t — wash the turkey before popping it in the oven on Thanksgiving Day. They say that could spread the germs lurking on your turkey in the kitchen sink or nearby food. But it’s been a challenge trying to convince cooks to stop rinsing off raw poultry. “If your mother did it and your grandmother did it, and suddenly the (government) says not to wash your turkey, you may take some time to adjust,” said Drusilla Banks, who teaches food sanitation for the University ...
CONCLUSION: All the three methods have high accuracy for the diagnosis of H. pylori infection in the Vietnamese population with gastritis and gastric ulcers. These tests can be employed in the clinical settings for the Vietnamese population. CLO test should be used in combination with the other tests to reduce false-negative results. PMID: 31759377 [PubMed - in process]