Mycobacterial Trehalose 6,6'-Dimycolate Induced M1-Type Inflammation.

Mycobacterial Trehalose 6,6'-Dimycolate Induced M1-Type Inflammation.
Am J Pathol. 2019 Nov 14;:
Authors: Nguyen TKT, d'Aigle J, Chinea L, Niaz Z, Hunter RL, Hwang SA, Actor JK
Abstract
Murine models of Mycobacterium tuberculosis (Mtb) infection demonstrate progression of M1-like (pro-inflammatory) and M2-like (anti-inflammatory) macrophage morphology following primary granuloma formation. The Mtb cell wall cording factor, trehalose 6,6'-dimycolate (TDM), is a physiologically-relevant and useful molecule for modeling early macrophage-mediated events during establishment of the tuberculosis-induced granuloma pathogenesis. Here we show that TDM is a major driver of the early M1-like macrophage response as seen during initiation of the granulomas of primary pathology. Pro-inflammatory cytokines tumor necrosis factor-α, interleukin (IL)-1β, IL-6, and IL-12p40 are produced in lung tissue after administration of TDM to mice. Furthermore, CD11b+CD45+ macrophages with a high surface expression of the M1-like markers CD38 and CD86 were found present in regions of pathology in lungs of mice at 7 days post TDM introduction. Conversely, only low phenotypic marker expression of M2-like markers CD206 and EGR-2 were present on macrophages. These findings suggest that TDM plays a role in establishment of the M1-like shift in the microenvironment during primary tuberculosis.
PMID: 31734231 [PubMed - as supplied by publisher]

Source: The American Journal of Pathology - November 13, 2019 Category: Pathology Authors: Nguyen TKT, d'Aigle J, Chinea L, Niaz Z, Hunter RL, Hwang SA, Actor JK Tags: Am J Pathol Source Type: research