Genetic characterization of three novel chicken parvovirus strains based on analysis of their coding sequences.

In this study, we obtained the nucleotide (nt) sequences of all genomic coding regions of three ChPVs by PCR using 13 primer sets, and deduced the amino acid (aa) sequences from the nt sequences. The non-structural protein 1 (NS1) gene of the three ChPVs showed 95.0 to 95.5% nt sequence identity and 96.5 to 98.1% aa sequence identity to those of NS1 from the ABU-P1 strain, respectively, and even higher nt and aa similarities to one another. The viral proteins (VP) gene was more divergent between the three ChPV Korean strains and ABU-P1, with 88.1 to 88.3% nt identity and 93.0% aa identity. Analysis of the putative tertiary structure of the ChPV VP2 protein showed that variable regions with less than 80% nt similarity between the three Korean strains and ABU-P1 occurred in large loops of the VP2 protein believed to be involved in antigenicity, pathogenicity, and tissue tropism in other parvoviruses. Based on our analysis of full-length coding sequences, we discovered greater variation in ChPV strains than reported previously, especially in partial regions of the VP2 protein. PMID: 25510852 [PubMed - as supplied by publisher]
Source: Avian Pathology - Category: Pathology Authors: Tags: Avian Pathol Source Type: research