Nuclear magnetic resonance based structure of the protoberberine alkaloid coralyne and its self-association by spectroscopy techniques

Publication date: Available online 26 September 2019Source: Journal of Pharmaceutical AnalysisAuthor(s): Kumar Padmapriya, Ritu BarthwalAbstractCoralyne is an important alkaloid due to its anti-cancer and other medicinal properties. It targets DNA in cells and acts as human topoisomerase-I poison, telomerase inhibitor and nucleic acid intercalator. It has high tendency to undergo self-association, which is a matter of concern for therapeutic applications. The understanding of its interaction with DNA requires precise knowledge of chemical shifts in Nuclear Magnetic Resonance (NMR) spectra besides self-association. The present study is the first report of a complete assignment of all 1H/13C resonances in NMR spectra of coralyne in DMSO-d6 using one dimensional 1H/13C and two dimensional NMR experiments. The chemical shift of all proton and several 13C resonances have also been obtained in D2O and ethanol-d6. The same has been calculated using Density Functional Theory (DFT). NMR spectra of coralyne show upfield shift of 0.6–1.2 ppm in aromatic ring protons suggesting stacking interactions. Apart from 11 intra molecular NOE cross peaks in 2D 1H–1H ROESY spectra, 3 short distance NOE correlations, H6–10OCH3, H5–10OCH3 and H12–16CH3, give direct independent evidence of the formation of a stacked dimer. The absorbance, fluorescence, circular dichroism and fluorescence lifetime experiments conducted in the present investigations corroborate results o...
Source: Journal of Pharmaceutical Analysis - Category: Drugs & Pharmacology Source Type: research

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Source: Cancers - Category: Cancer & Oncology Authors: Tags: Article Source Type: research
Publication date: Available online 26 September 2019Source: Journal of Pharmaceutical AnalysisAuthor(s): Kumar Padmapriya, Ritu BarthwalAbstractCoralyne is an important alkaloid due to its anti cancer and other medicinal properties. It targets DNA in cells and acts as human topoisomerase-I poison, telomerase inhibitor and nucleic acid intercalator. It has high tendency to undergo self association, which is a matter of concern for therapeutic applications. The understanding of its interaction with DNA requires precise knowledge of chemical shifts in Nuclear Magnetic Resonance (NMR) spectra besides self association. The pres...
Source: Journal of Pharmaceutical Analysis - Category: Drugs & Pharmacology Source Type: research
 Chrisa Hickey’s journey into mental health advocacy started when her son, Tim, was diagnosed with very early onset schizophrenia after being admitted to a psychiatric hospital for the first time at the age of 11.  He had been showing symptoms for years and had received a half dozen different diagnoses. His family was desperately looking for answers. Tim’s illness took a toll on the entire family, which was only exacerbated by the lack of information and resources available to them.  In America, fewer than 100 children per year are diagnosed with very early onset schizophrenia. Chrisa had to find ...
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Source: Scandinavian Journal of Work, Environment and Health - Category: Occupational Health Tags: Commentary Source Type: research
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Source: Scandinavian Journal of Work, Environment and Health - Category: Occupational Health Authors: Tags: Scand J Work Environ Health Source Type: research
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