Preparation of chitosan-based composites with urethane cross linkage and evaluation of their properties for using as wound healing dressing

Publication date: Available online 16 November 2019Source: Carbohydrate PolymersAuthor(s): Hossein Rahmani, Seyed Heydar Mahmoudi Najafi, Alireza Ashori, Marzieh Arab Fashapoyeh, Farzaneh Aziz Mohseni, Sara TorkamanAbstractIn this research, biocomposite films containing chitosan (CS), polyvinyl alcohol (PVA) and polyvinyl pyrrolidone (PVP), with different ratios, have been provided. The effects of adding hexamethylene 1, 6-di(aminocarboxysulfonate) (HMDACS) as cross linker and the formation of urea linkage on mechanical properties such as tensile strength and elongation and dynamic-mechanical properties such as storage modulus and tan δ were studied. Also, the antibacterial properties of the composites were investigated by viable bacterial cell counting and compared in reducing the bacterial growth. The final results showed the composite containing CS (50 wt%), PVA (30 wt%), PVP (20 wt%) and HMDACS (2 wt%) had the highest mechanical properties. Scanning electron microscopy (SEM) micrographs confirmed uniform distribution of components in the polymer matrix. In general, In general, low contact angle values revealed the hydrophilicity of the prepared films. It was found that the composites made by combining CS, PVA and PVP at concentration of 50, 25, 25 wt% (A3) and 60, 20, 20 wt% (B4), cross-linked with 2 wt% HMDACS, had the best antibacterial activity against Escherichia coli and Staphylococcus aureus, hence they can be u...
Source: Carbohydrate Polymers - Category: Biomedical Science Source Type: research

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In conclusion, potential biological applications of PF-SeNPs were explored by antibacterial, antifungal, antioxidant and biocompatibility assays. Materials and Methods Chemicals and Reagents Sodium selenite (98%), 2,2-diphenyl-1-picrylhydrazyl (DPPH), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) (MTT), Tween 80, methanol, acetate buffer, tetracycline, nystatin, rhodamine 123, caspase-3 kit, Dulbecco’s modified Eagle media (DMEM), Folin-Ciocalteu reagent, 2,2-azinobis-(3-ethylbenzothiazoline-6-sulfonate) (ABTS), Dulbecco’s phosphate buffered saline pH 7.4 (DPBS) and fetal bovine serum (FBS) w...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Publication date: Available online 18 April 2019Source: Journal of Industrial and Engineering ChemistryAuthor(s): Katarina Nešović, Ana Janković, Aleksandra Perić-Grujić, Maja Vukašinović-Sekulić, Tamara Radetić, Ljiljana Živković, Soo-Jin Park, Kyong Yop Rhee, Vesna Mišković-StankovićAbstractSilver nanoparticles (AgNPs) were synthesized by in situ electrochemical reduction of Ag+ ions in poly(vinyl alcohol)/chitosan/graphene (PVA/CHI/Gr) hydrogel matrices with different concentrations of chitosan. The physicochemical properties of nanocomposite hydrogels were investigated by ultraviolet-vis...
Source: Journal of Industrial and Engineering Chemistry - Category: Chemistry Source Type: research
In this study, we sought to investigate whether SH0165 (serovar 5, high-virulent strain) and HN0001 (serovar 6, non-virulent strain) infection induces autophagy and the specific role of autophagy in bacterial invasion and inflammation during H. parasuis infection. Moreover, we explored the mechanism underlying autophagy regulated inflammation through inflammatory signaling cascades during H. parasuis infection. This observation could provide useful information for further understanding the role of autophagy in H. parasuis infection and improve our knowledge of new strategies against this pathogen. Materials and Methods B...
Source: Frontiers in cellular and infection microbiology - Category: Microbiology Source Type: research
In this study, we applied a label-free shotgun proteomic approach to characterize and quantify the NCDO 2118 proteome in response to variations of temperature and oxygen bioavailability, which constitute the environmental conditions found by this bacterium during its passage through the host gastro-intestinal tract and in other industrial processes. From this proteomic analysis, a total of 1,284 non-redundant proteins of NCDO 2118 were characterized, which correspond to approximately 54% of its predicted proteome. Comparative proteomic analysis identified 149 and 136 proteins in anaerobic (30°C and 37°C) and non-ae...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Abstract The agro-industrial waste obtained after the isolation of bio-constituents from ginger is available in abundance. In the present study, the effective isolation of ginger nanofibers (GNF) through acid hydrolysis was carried out to get cellulose nanofibers with 100 to 200 nm width by high pressure homogenization. Bionanocomposites were also prepared by reinforcing different ratios of (1% to 7%) GNF with chitosan (CS) and polyvinyl alcohol (PVA) matrices by solvent cast method and the 5% GNF with CS and PVA resulted a high mechanical strength composites than others. The surface morphology and structural an...
Source: International Journal of Biological Macromolecules - Category: Biochemistry Authors: Tags: Int J Biol Macromol Source Type: research
g Huawei He Silk sericin (SS) is a type of natural macromolecular protein with excellent hydrophilicity, biocompatibility and biodegradability, but also has very poor mechanical properties. To develop sericin-based wound dressings, we utilized polyvinyl alcohol (PVA) to reinforce the mechanical property of sericin by blending PVA and sericin, then modified zinc oxide nanoparticles (ZnO NPs) on SS/PVA film with the assistance of polydopamine (PDA) to endow SS/PVA film with antibacterial activity. Scanning electron microscopy, energy dispersive spectroscopy and X-ray powder diffraction demonstrated ZnO NPs were well gr...
Source: Molecules - Category: Chemistry Authors: Tags: Article Source Type: research
Publication date: Available online 16 August 2018Source: Journal of Food and Drug AnalysisAuthor(s): Geetika Wadhwa, Sunil Kumar, Vineet Mittal, Rekha RaoAbstractBabchi essential oil (BEO) is a valuable essential oil reported to possess a variety of biological activities such as antitumor, anti inflammatory, immunomodulatory, antioxidant, antifungal and antibacterial properties. Due to its anti-microbial properties, this oil possesses an immense potential for the treatment of dermatological disorders. Further, it has minimal tendency to develop resistance, a common issue with most of the antibiotics. However, its highly vi...
Source: Journal of Food and Drug Analysis - Category: Food Science Source Type: research
Publication date: 1 August 2017 Source:Materials Science and Engineering: C, Volume 77 Author(s): J.J. Ahire, D.D. Robertson, A.J. van Reenen, L.M.T. Dicks Surfactin-loaded polyvinyl alcohol (PVA) nanofibers were spun using gravity electrospinning. Scanning electron microscopy (SEM) images showed that nanofibers spun with surfactin are free from bead formation and uniform in diameter. The average nanofiber diameters were decreased (273±39nm, 259±39nm and 217±33nm) with increasing levels of surfactin (0.5, 1.0 and 1.5%, w/v) into PVA (10%, w/v). The 10% (w/v) PVA had average fiber diameter of 303&plusm...
Source: Materials Science and Engineering: C - Category: Materials Science Source Type: research
Publication date: Available online 9 March 2017 Source:Journal of Photochemistry and Photobiology B: Biology Author(s): V. Sai Saraswathi, Neethu Kamarudheen, K.V. Bhaskara Rao, K. Santhakumar The investigation was conducted to analyse the bioactive compounds from the leaf extracts of L. speciosa by GC–MS. The extracts were screened for antibacterial and antibiofilm activities against potential clinical strains. The bioactive compounds from the leaves of L. speciosa were extracted by soxhlet continuous extraction method and their chemical composition was analysed by Gas Chromatography–Mass Spectroscopy (GC&nda...
Source: Journal of Photochemistry and Photobiology B: Biology - Category: Speech Therapy Source Type: research
This report, for the first time indicates simple coaxial spinneret electrospinning technique for the fabrication of biomedicated core-shell fine curcumin (Cur) nanofiber mats with a polymer-free core which can prevent wound infections. Polyvinyl alcohol (PVA) and Chitosan (CS) were used as the shell of nanofibers and curcumin, a natural drug with high antimicrobial activity, was loaded into the core of nanofibers (without any polymer) for the enhancement of bioactivity and bioavailability. Scanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM) images show that the prepared nanofibers were fine and un...
Source: Polymer - Category: Chemistry Source Type: research
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