A glance of the blood stage transcriptome of a Southeast Asian Plasmodium ovale isolate

by Awtum M. Brashear, Wanlapa Roobsoong, Faiza A. Siddiqui, Wang Nguitragool, Jetsumon Sattabongkot, Margarita M. L ópez-Uribe, Jun Miao, Liwang CuiPlasmodium ovale accounts for a disproportionate number of travel-related malaria cases. This parasite is understudied since there is a reliance on clinical samples. We collected aP.ovale curtisi parasite isolate from a clinical case in western Thailand and performed RNA-seq analysis on the blood stage transcriptomes. Using bothde novo assembly and alignment-based methods, we detected the transcripts for 6628 out of 7280 annotated genes. For those lacking evidence of expression, the vast majority belonged to the PIR and STP1 gene families. We identified new splicing patterns for over 2500 genes, and mapped at least one untranslated region for over half of all annotated genes. Our analysis also detected a notable presence of anti-sense transcripts for over 10% ofP.ovale curtisi genes. This transcriptomic analysis provides new insights into the blood-stage biology of this neglected parasite.
Source: PLoS Neglected Tropical Diseases - Category: Tropical Medicine Authors: Source Type: research

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Date: Tuesday, 01 07, 2020; Speaker: Daniel Neafsey, Assistant Professor, Harvard T.H. Chan School of Public Health; 5625 Fishers Lane; 5th Floor Conference Room
Source: NIH Calendar of Events - Category: American Health Source Type: events
Contributors : Lauren M Smith ; Francis Motta ; Garima Chopra ; J K Moch ; Robert R Nerem ; Bree Cummins ; Kimberly E Roche ; Christina M Kelliher ; Adam R Leman ; John Harer ; Tomas Gedeon ; Norman C Waters ; Steven B HaaseSeries Type : Expression profiling by high throughput sequencingOrganism : Plasmodium falciparumThe blood-stage infection of the malaria parasite, Plasmodium falciparum, exhibits a 48-hour developmental cycle that culminates in the synchronous release of parasites from red blood cells, triggering 48-hour fever cycles in the host. This cycle could be driven extrinsically by host circadian processes, or b...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Plasmodium falciparum Source Type: research
Malaria infection can present with a wide variety of symptoms, ranging from mild to severe. Plasmodium falciparum isolates in uncomplicated and severe malaria infections may have different parasite genetic profil...
Source: Malaria Journal - Category: Infectious Diseases Authors: Tags: Research Source Type: research
arimantas Čėnas With the aim to clarify the mechanism(s) of action of nitroaromatic compounds against the malaria parasite Plasmodium falciparum, we examined the single-electron reduction by P. falciparum ferredoxin:NADP+ oxidoreductase (PfFNR) of a series of nitrofurans and nitrobenzenes (n = 23), and their ability to inhibit P. falciparum glutathione reductase (PfGR). The reactivity of nitroaromatics in PfFNR-catalyzed reactions increased with their single-electron reduction midpoint potential (E17). Nitroaromatic compounds acted as non- or uncompetitive inhibitors towards PfGR with respect to NADPH and glutathione...
Source: Molecules - Category: Chemistry Authors: Tags: Article Source Type: research
Natural killer (NK) cells are known to be activated during malaria infection, exhibiting both cytokine production and cytotoxic functions. However, NK cells are heterogeneous in their expression of surface activatory and inhibitory receptors which may influence their response to malaria parasites. Here, we studied the surface marker profile and activation dynamics of NK cells during a Controlled Human Malaria Infection in 12 healthy volunteers. Although there was significant inter-patient variability in timing and magnitude of NK cell activation, we found a consistent and strong increase in expression of the activatory rec...
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research
Conclusions/SignificanceOur data indicate that howler monkeys act as the main reservoir for the Atlantic forest human malarial parasites in RJ and other sites in Southeast Brazil and reinforce its zoonotic characteristics.
Source: PLoS Neglected Tropical Diseases - Category: Tropical Medicine Authors: Source Type: research
Authors: Bartra-More CA, Ramos-Castillo J, Mendoza-Bautista RC, Galindo-Cabello NR, Gebol-Cahuaza M, Chirinos-Palomino FJ Abstract Timely diagnosis of malaria is a strategy proposed by the World Health Organization to reduce malaria morbidity and mortality. A study was conducted to assess performance in microscopic diagnosis of malaria in the network of laboratories under the National Malaria Reference Laboratory of the National Institute of Health between 2012 and 2017. In the years of study, the laboratories obtained a rating of "acceptable" in the diagnosis of Plasmodium by 38.4%, 43.7%, 60.0%, 83.3%, ...
Source: Revista Peruana de Medicina de Experimental y Salud Publica - Category: International Medicine & Public Health Tags: Rev Peru Med Exp Salud Publica Source Type: research
Conclusion: The mosquito collected and mapped can be considered for transmission of malaria and filariasis in the region. Bearing in mind the results of niche modeling for vector species, more studies on vectorial capacity and resistance status to different insecticides of these species are recommended. PMID: 31803777 [PubMed]
Source: Iranian Journal of Arthropod-Borne Diseases - Category: Infectious Diseases Tags: J Arthropod Borne Dis Source Type: research
Conclusion: Some investigations have reported An. martinius as a member of sibling species of An. sacharovi among Iranian Anopheles genus; while based on our study, there was no evidence of the presence of this species in north and northwest of Iran. PMID: 31803774 [PubMed]
Source: Iranian Journal of Arthropod-Borne Diseases - Category: Infectious Diseases Tags: J Arthropod Borne Dis Source Type: research
Malaria is caused by unicellular apicomplexan parasites of the genus Plasmodium, which includes the major human parasite Plasmodium falciparum. The complex cycle of the malaria parasite in both mosquito and human hosts has been studied extensively. There is tight control of gene expression in each developmental stage, and at every level of gene synthesis: from RNA transcription, to its subsequent translation, and finally post-translational modifications of the resulting protein. Whole-genome sequencing of P. falciparum has laid the foundation for significant biological advances by revealing surprising genomic information. ...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
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