Sylvatic dengue virus type 4 in Aedes aegypti and Aedes albopictus mosquitoes in an urban setting in Peninsular Malaysia

by Nur Alia Johari, Kenny Voon, Shen Yung Toh, Lokman Hakim Sulaiman, Ivan Kok Seng Yap, Patricia Kim Chooi Lim Dengue fever is endemic in Malaysia, contributing to significant economic and health burden in the country.Aedes aegypti andAe.albopictus are the main vectors of the dengue virus (DENV), which circulates in sylvatic and human transmission cycles and has been present in Malaysia for decades. The study investigated the presence and distribution of DENV in urban localities in the Klang Valley, Peninsular Malaysia. A total of 364Ae.aegypti and 1,025Ae.albopictus larvae, and 10Ae.aegypti and 42Ae.albopictus adult mosquitoes were screened for the presence of DENV. In total, 31 (2.2%) samples were positive, of which 2Ae.albopictus larvae were co-infected with two serotypes, one with DENV-2 and DENV-3 and the other with DENV-3 and DENV-4. Phylogenetic analysis determined that the isolates belonged to DENV-1 genotype I (1Ae.aegypti adult), DENV-2 (1Ae.albopictus larva), DENV-3 genotype V (3Ae.aegypti larvae and 10Ae.albopictus larvae) and DENV-4 genotype IV (6Ae.aegypti larvae and 12Ae.albopictus larvae), a sylvatic strain of DENV-4 which was most closely related with sylvatic strains isolated from arboreal mosquitoes and sentinel monkeys in Peninsular Malaysia in the 1970s. All four DENV serotypes were co-circulating throughout the study period. The detection of a sylvatic strain of DENV-4 inAe.aegypti andAe.albopictus mosquitoes in urban areas in Peninsular Malaysia highl...
Source: PLoS Neglected Tropical Diseases - Category: Tropical Medicine Authors: Source Type: research

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On 1 May 2019, in response to increasing numbers of dengue fever cases in Pakistan and India, health authorities in Afghanistan heightened monitoring for the disease. As part of this increased vigilance, the Central Public Health Laboratory (CPHL) in Kabul began to broaden its investigation for possible cases of the disease, such as reviewing samples that tested negative for Crimean-Congo Hemorrhagic Fever (CCHF) to see if they were positive for dengue. The laboratory performed differential diagnosis and tests on 40 samples that had tested negative for Crimean-Congo Hemorrhagic Fever (CCHF). Between 1 October to 4 December...
Source: WHO Disease Outbreaks - Category: Infectious Diseases Tags: news Source Type: news
Vector-Borne and Zoonotic Diseases, Ahead of Print.
Source: Vector-Borne and Zoonotic Diseases - Category: Infectious Diseases Authors: Source Type: research
Conclusion: Marked difference noted between two groups in Aptt prolongation, INR elevation, ALT level, S.Fibrinogen, CCHF IgM positivity, major bleeding symptoms and CNS involvement while no such major difference seen in other parameters like platelet count, total WBC count and other clinical parameters. Further study with large sample size from indian patients needed to confirm above findings. PMID: 31801328 [PubMed - in process]
Source: Journal of the Association of Physicians of India - Category: General Medicine Tags: J Assoc Physicians India Source Type: research
Abstract Dengue fever is a highly endemic arthropod-borne viral disease in the tropical and sub-tropical countries and is rapidly becoming a global threaten. Diagnosis has been conducted by either traditional serological methods or molecular biological techniques. However, these methods are either labor-intensive, time-consuming or with multiple steps, which are not suitable for high throughput detection of large quantity of samples. In the current study, a novel, rapid, no-wash one-step amplified luminescent proximity homogenous assay-linked immunosorbent assay (AlphaLISA) was developed and optimized for the diag...
Source: Journal of Virological Methods - Category: Virology Authors: Tags: J Virol Methods Source Type: research
L, Ant TH, Herd CS, Hasnor AH, AbuBakar R, Hapsah DM, Khadijah K, Kamilan D, Lee SC, Paid YM, Fadzilah K, Topek O, Gill BS, Lee HL, Sinkins SP Abstract Dengue has enormous health impacts globally. A novel approach to decrease dengue incidence involves the introduction of Wolbachia endosymbionts that block dengue virus transmission into populations of the primary vector mosquito, Aedes aegypti. The wMel Wolbachia strain has previously been trialed in open releases of Ae. aegypti; however, the wAlbB strain has been shown to maintain higher density than wMel at high larval rearing temperatures. Releases of Ae. aegypt...
Source: Current Biology - Category: Biology Authors: Tags: Curr Biol Source Type: research
Dengue is a vector-borne disease with a significant impact on Latin America. According to the annual report of the Pan American Health Organization, Colombia is the main contributor of dengue in the Andean region, and the second in Latin America, with an incidence of 321.4 cases per 100,000 inhabitants in 2016 (WHO, 2017). Colombia is hyperendemic (Guzm án and Kouri, 2003; Pérez et al., 2016), and approximately 65% of Colombian municipalities have ecological conditions that favor the sustained circulation of Aedes aegypti and the transmission of dengue virus (DENV) (Díaz-Quijano et al., 2018).
Source: International Journal of Infectious Diseases - Category: Infectious Diseases Authors: Source Type: research
Vector-Borne and Zoonotic Diseases, Ahead of Print.
Source: Vector-Borne and Zoonotic Diseases - Category: Infectious Diseases Authors: Source Type: research
We report a case of a man with ESRF with a delayed diagnosis of dengue viral infection, who admitted at the critical phase of the illness. Despite that, he managed to recover uneventfully with tailored management in his fluid replacement, medication adjustment, and dialysis prescription. Hence, a high index of suspicion is needed when an ESRF patient presents with fever in a dengue-endemic area. A multidisciplinary approach with good collaboration between physician and nephrologist is needed to ensure the best outcome. Renal replacement therapy and medications should be individually tailored according to the patient's clin...
Source: Infectious Diseases in Clinical Practice - Category: Infectious Diseases Tags: Review Articles Source Type: research
Dengue is a mosquito-borne viral disease caused by dengue virus (DENV). The disease is endemic to more than 100 countries with 390 million dengue infections per year. Humoral immune responses during primary and secondary DENV infections are well-investigated. However, the impact of DENV infection on B cell subsets and their antibody-independent functions are not well-documented. Through this study, we aimed to define the distribution of B cell subsets in the acute phase of DENV infection and characterize the effect of DENV infection on B cell functions such as differentiation into memory and plasma cells and cytokine produ...
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research
by Valerie A. Paz-Soldan, Amy C. Morrison, Heng Sopheab, Julia Schwarz, Karin M. Bauer, Jennie L. Mckenney, Chhorvann Chhea, Vonthanak Saphonn, Dyna Khuon, Robert D. Hontz, Pamina M. Gorbach In 2012, the U.S. Defense Threat Reduction Agency Joint Science and Technology Office initiated a program to develop novel point-of-need diagnostic devices for surveillance of emerging infectious diseases including dengue, malaria, plague, and melioidosis. Prior to distribution of devices to obser ve their correct use among community members in Iquitos, Peru, and Phnom Penh, Cambodia, research was conducted to: 1) assess acceptability...
Source: PLoS Neglected Tropical Diseases - Category: Tropical Medicine Authors: Source Type: research
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