Interactions of free-living amoebae with the rice fungal pathogen, Rhizoctonia solani

Rhizoctonia solani is a soil-borne fungal pathogen of many important crop plants. In rice, R. solani causes sheath blight disease, which results in devastating grain yield and quality losses. Few methods are avai...
Source: BMC Research Notes - Category: Research Authors: Tags: Research note Source Type: research

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Purpose To assess the prevalence of free-living Acanthamoeba in domestic tap-water in the greater Sydney region in NSW, Australia, and determine any seasonal variation in prevalence. We hypothesized that higher temperatures in summer would generate a higher abundance of food for the amoeba, and therefore a greater prevalence of Acanthamoeba would be found in summer compared to winter.
Source: Contact Lens and Anterior Eye - Category: Opthalmology Authors: Source Type: research
Purpose Microbial contamination of contact lens storage cases is associated with corneal disease ranging from sterile keratitis to sight threatening infections including bacterial and Acanthamoeba keratitis. Given the increasing association between water contact and contact lens-related disease, this study aimed to explore the effect of water contact and storage case contamination in habitual daily wear contact lens users.
Source: Contact Lens and Anterior Eye - Category: Opthalmology Authors: Source Type: research
Authors: Oliveira-Ferreira C, Leuzinger-Dias M, Tavares-Ferreira J, Torrão L, Falcão-Reis F Abstract The microbiological profile of infectious keratitis has shown great differences across the world. Due to the continuous shifting trends in microbiological profile and antibiotic resistance patterns reported in several studies, constant local updates are crucial to provide an adequate treatment. The propose of this study was to analyze the incidence of infectious keratitis, possible changing trends in microbiological profile, and bacteria sensitivity to commonly used antibiotics, in our tertiary center,...
Source: Journal of Ophthalmology - Category: Opthalmology Tags: J Ophthalmol Source Type: research
[Acanthamoeba endophthalmitis-a case report]. Ophthalmologe. 2019 Nov 25;: Authors: Pascha J, Frings A, Walochnik J, Wissiak E, Dirisamer M, Daas L, Ardjomand N Abstract Diagnosis of acanthamoeba keratitis is often a clinical challenge. In most cases it is primarily confused with herpes keratitis. A coinfection with bacteria or fungi can also lead to a delayed diagnosis and initiation of treatment. A few cases reported the detection of acanthamoeba in the anterior chamber. In the case of endophthalmitis without the detection of bacteria or fungi acanthamoeba should therefore always be c...
Source: Der Ophthalmologe - Category: Opthalmology Authors: Tags: Ophthalmologe Source Type: research
Authors: Tananuvat N, Techajongjintana N, Somboon P, Wannasan A Abstract Acanthamoeba keratitis (AK) is a rare sight-threatening corneal infection, often reporting from contact lens wearers. An asymptomatic human immunodeficiency virus (HIV)-infected Thai male without history of contact lens use complained foreign body sensation at his left eye during motorbike riding. He had neither specific keratitis symptoms nor common drugs responding, which contributed to delayed diagnosis. By corneal re-scraping, Acanthamoeba-like cysts were detected by calcofluor white staining and agar culture. The etiological agent obtaine...
Source: Korean Journal of Parasitology - Category: Parasitology Tags: Korean J Parasitol Source Type: research
Acanthamoeba is well known to produce a blinding keratitis and serious brain infection known as encephalitis. Effective treatment is problematic, and can continue up to a year, and even then, recurrence can ensue...
Source: Parasites and Vectors - Category: Microbiology Authors: Tags: Research Source Type: research
In conclusion, our results show that the growth-stage-specific expression level of CsrA is dually regulated by ClpP-dependent proteolysis at both the transcription and protein levels during the biphasic life cycle of L. pneumophila.
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Since the discovery of mimivirus, numerous giant viruses associated with free-living amoebae have been described. The genome of giant viruses can be more than 2.5 megabases, and virus particles can exceed the ...
Source: Virology Journal - Category: Virology Authors: Tags: Review Source Type: research
Abstract Miltefosine is an alkylphosphocholine compound used primarily for treatment of leishmaniasis that also demonstrates in vitro and in vivo anti-amebic activity against Acanthamoeba species. As such, recommendations for treatment of amebic encephalitis generally include miltefosine therapy. Data supports a minimum amebicidal concentration of at least 16 mcg/mL is required for most Acanthamoeba species. Although there is a high level of mortality associated with amebic encephalitis, a paucity of data regarding miltefosine levels in the plasma and cerebrospinal fluid in vivo exists in the literature. We found ...
Source: Antimicrobial Agents and Chemotherapy - Category: Microbiology Authors: Tags: Antimicrob Agents Chemother Source Type: research
The objective of this study was to evaluate the behavior of Acanthamoeba mauritaniensis, a nonpathogenic amoeba. We analyzed the cytopathic effect of A. mauritaniensis on RCE1(5 T5) and MDCK cells and compared it to that of Acanthamoeba castellanii. A partial biochemical characterization of proteases was performed in total crude extracts (TCE) and conditioned medium (CM). Finally, we evaluated the effect of proteases on tight junction (TJ) proteins and the transepithelial electrical resistance of MDCK cells. The results showed that this amoeba can induce substantial damage to RCE1(5T5) and MDCK cells. Moreover, the zymog...
Source: Parasitology International - Category: Parasitology Source Type: research
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