Novel spider web trap approach based on chitosan/cellulose nanocrystals/glycerol membrane for the detection of Escherichia coli O157:H7 on food surfaces.

Novel spider web trap approach based on chitosan/cellulose nanocrystals/glycerol membrane for the detection of Escherichia coli O157:H7 on food surfaces. Int J Biol Macromol. 2019 Nov 11;: Authors: Baraketi A, D'Auria S, Shankar S, Fraschini C, Salmieri S, Menissier J, Lacroix M Abstract The aim of this study was to develop a novel approach allowing simultaneous enrichment as well as specific and fast detection of Escherichia coli O157:H7 by indirect ELISA using optimized support membrane based on chitosan (CHI), cellulose nanocrystals (CNCs), and glycerol (GLY). Therefore, combining the step of the capture of the pathogen and enrichment steps for the microbial growth led to a high detection signal at a low inoculation level without cross-reaction with Pseudomonas and Salmonella strains. The detection was performed by varying incubation periods and different level of inoculations. The signal of detection in samples incubated with the chitosan-based support reinforced with CNCs and directly from E.coli O157:H7 bacterial culture was much higher as compared to CNCs-free support with cell-free supernatant samples. The CCG support reinforced with 0.6 % CNCs improved the detection signal of E. coli O157:H7 by 25 % compared to control. The whole bacterial culture showed a higher immobilization signal than unfiltered and cell-free supernatant. The spider web trap approach (SWTA) detect E. coli O157:H7 after only 4 h of enrichment compared to 24 h with convention...
Source: International Journal of Biological Macromolecules - Category: Biochemistry Authors: Tags: Int J Biol Macromol Source Type: research

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The CDC says eight people have contracted E. coli in three states from a pre-packaged salad kit while the FDA announced a recall of a pre-cut fruit salad mix that sickened 33 with salmonella.
Source: the Mail online | Health - Category: Consumer Health News Source Type: news
Authors: Kawanishi M, Hisatomi Y, Oda Y, Shimohara C, Tsunematsu Y, Sato M, Hirayama Y, Miyoshi N, Iwashita Y, Yoshikawa Y, Sugimura H, Mutoh M, Ishikawa H, Wakabayashi K, Yagi T, Watanabe K Abstract Colibactin is a polyketide-peptide genotoxin produced by enteric bacteria such as E. coli, and is considered to contribute to the development of colorectal cancer. We previously isolated E. coli strains from Japanese colorectal cancer patients, and in the present study we investigated the genotoxic potency of the colibactin-producing (clb+) E. coli strains that carry the polyketide synthases "pks" gene cluste...
Source: Journal of Toxicological Sciences - Category: Toxicology Tags: J Toxicol Sci Source Type: research
In this study, we evaluated the efficacy of our two previously developed real time-quantitative PCR (qPCR) assays for the detection of swine-associated Bacteroidales genetic markers (gene 1–38, gene 3–53) in the Yangtze Delta watershed of southeastern China. The results indicated that the gene 1–38 and 3-53 markers exhibited high accuracy (92.5%, 91.7% conditional probability, respectively) in detecting Bacteroidales spp. in water samples. According to binary logistic regression (BLR), these two swine-associated markers were well correlated (P 
Source: Journal of Environmental Sciences - Category: Environmental Health Source Type: research
Abstract The plasmid-mediated quinolone resistance (PMQR) genes have changed the resistance pattern to quinolones, especially among Enterobacteriales. The dissemination of these genes in Latin American countries, where the prescription of fluoroquinolones is high, has been described in several studies; however, no review of the impact of PMQR in this continent has been conducted. This review summarized current knowledge about the circulation of PMQR among Enterobacteriales in Latin American. After the search and selection, 61 articles were included in the study. Most of studies reported PMQR genes among Enterobact...
Source: Molecular Biology Reports - Category: Molecular Biology Authors: Tags: Mol Biol Rep Source Type: research
Publication date: Available online 5 December 2019Source: Research in Veterinary ScienceAuthor(s): Marta Perez-Sancho, Teresa García-Seco, Concepción Porrero, Nerea García, Susana Gomez-Barrero, Jose María Cámara, Lucas Domínguez, Julio ÁlvarezAbstractFeral pigeons have increased in urban settings worldwide becoming a potential health risk for humans and other animals. Control and surveillance programs are essential to prevent the possible transmission of zoonotic pathogens carried by pigeons. A surveillance program was carried out in Madrid City (Spain) during 2005–20...
Source: Research in Veterinary Science - Category: Veterinary Research Source Type: research
Publication date: Available online 2 December 2019Source: Journal of Molecular StructureAuthor(s): R. Rama, S. MeenakshiAbstractA novel Aliquat 336 based functionalized ionic liquid, methyltrioctylammonium naphthylacetate ([A336]+[NAA]-) have been synthesized and characterized using IR, UV, 1H NMR and Raman spectroscopy techniques. The prepared IL was tested for antimicrobial properties and chromium (VI) extraction. Anti-bacterial test was carried out towards five types of bacteria namely Staphylococcus aureus, Escherichia coli, Pseudomonas aeroginosa, Bacillus subtilis and Salmonella typhi. The antifungal test was done ou...
Source: Journal of Molecular Structure - Category: Molecular Biology Source Type: research
Fluoroquinolones are used to treat serious bacterial infections, including those caused by Escherichia coli and Salmonella enterica. The emergence of plasmid-mediated quinolone resistance (PMQR) represent a new challenge to the successful treatment of Gram-negative infections. As part of a long-term strategy to generate a reference database of closed plasmids from antimicrobial resistant foodborne bacteria, we performed long-read sequencing of 11 E. coli isolates from retail meats that were non-susceptible to ciprofloxacin. Each of the isolates had PMQR genes, including qnrA1, qnrS1, and qnrB19. The four qnrB19 genes were ...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
This study quantified the association of rodent fruit damage and the microbiological quality of irrigation water on the risk of microbiological contamination of strawberries collected from 18 U-pick farms across five different districts in the Guangzhou metropolitan region of southern China. Fifty-four composite strawberries samples, with or without evidence of rodent or avian foraging damage (i.e., bitten), along with 16 irrigation water samples, were collected during the spring of 2014 and winter of 2015 from our cohort of 18 farms. Composite strawberry samples and irrigation water were analyzed for total coliforms, E. c...
Source: International Journal of Environmental Research and Public Health - Category: Environmental Health Authors: Tags: Article Source Type: research
Publication date: Available online 3 December 2019Source: LWTAuthor(s): Jin-Young Han, Won-Jae Song, Joo Hyun Kang, Sea C. Min, Sangheum Eom, Eun Jeong Hong, Seungmin Ryu, Seongbong Kim, Sangwoo Cho, Dong-Hyun KangAbstractWe evaluated the effect of plasma-activated water (PAW) on the populations of foodborne pathogens and food-spoilage microorganisms on Korean rice cake. PAW was produced by treating distilled water with two atmospheric dielectric-barrier discharges (51.7 W, 14.4 kHz, air discharge) for 20 min. The inactivation effect of PAW on indigenous microorganism and inoculated Escherichia coli O157:H7, Salmonel...
Source: LWT Food Science and Technology - Category: Food Science Source Type: research
Publication date: Available online 3 December 2019Source: Food Research InternationalAuthor(s): Elvira Frías, Yenea Iglesias, Avelino Alvarez-Ordóñez, Miguel Prieto, Montserrat González-Raurich, Mercedes LópezAbstractThe efficacy of Cold Atmospheric Pressure Plasma (CAPP) for the inactivation of Salmonella enterica serovar Enteritidis, Salmonella enterica serovar Typhimurium, Listeria monocytogenes and Escherichia coli O157:H7 on tofu was evaluated. The potential of using Plasma Activated Water (PAW) as an immersion solution for controlling microbial growth in tofu throughout its shelf-li...
Source: Food Research International - Category: Food Science Source Type: research
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