Interactions of Indole Alkaloids with Myoglobin: A Mass Spectrometry-based Spectrometric and Computational Method.

CONCLUSIONS: A new mass spectrometry-based integrated analysis method has been developed to successfully study the interactions of Mb and IAs extracted from Ophiorrhiza japonica. The experimental and calculation results have good consistency, revealing all of the four IA molecules could bind to Mb to form 1:1, 1:2 and 1:3 Mb-IA complexes, respectively. The order of binding ability of these IAs to Mb was Ophiorrhine B > Compound C > Ophiorrhine A > Compound D. CD and HDX-MS results indicated that binding with IAs destabilizes resulted of Mb. HDX-MS analysis suggests that Mb becomes more accessible to HDX, indicating that IAs binding destabilizes the structure of Mb. In addition, interacting with IAs affected the overall structure of Mb, ascribed to the decrease of α-helix content and less folding of backbone.
PMID: 31721336 [PubMed - as supplied by publisher]

Source: Rapid Communications in Mass Spectrometry : RCM - November 11, 2019 Category: Chemistry Authors: Li YW, Chi Q, Feng T, Xiao H, Li L, Wang X Tags: Rapid Commun Mass Spectrom Source Type: research