In Vitro Metabolism of Imidacloprid and Acetamiprid in Rainbow Trout and Rat.

In Vitro Metabolism of Imidacloprid and Acetamiprid in Rainbow Trout and Rat. Xenobiotica. 2019 Nov 14;:1-37 Authors: Kolanczyk RC, Tapper MA, Sheedy BR, Serrano JA Abstract 1. Providing an alternative to pyrethroids, organophosphates, and carbamates, the neonicotinoids are now the most widely used insecticides in the world. They are water soluble and relatively stable in soil and water which allows for run-off through surface waters and thus potentially impacting aquatic species and environments.2. While the mammalian metabolism of neonicotinoids has been studied extensively, there is a lack of understanding of their metabolism in fish species. The current study constitutes the first report of the metabolism of imidacloprid (IMI) and acetamiprid (AC) in rainbow trout.3. Formation of respective metabolites 5-hydroxy-imidacloprid and N-desmethyl-acetamiprid was conserved across orders of biological organization in both microsomal and liver slice assays.4. Michaelis-Menten kinetics were determined for the microsomal conversion of IMI to 5-hydroxy-imidacloprid in rainbow trout (Km =79.2 µM; Vmax =0.75 pmole/min/mg) and rat (Km =158.7 µM; Vmax =38.4 pmole/min/mg). Kinetics for the microsomal demethylation of AC to N-desmethyl-acetamiprid were determined in the rat (Km =70.9 µM; Vmax =4.10 pmoles/min/mg). N-desmethyl-acetamiprid was found in detectable but below quantifiable levels across the range of test concentrations which preclude...
Source: Xenobiotica - Category: Research Authors: Tags: Xenobiotica Source Type: research