Viruses, Vol. 11, Pages 1064: Erythromycin Estolate Inhibits Zika Virus Infection by Blocking Viral Entry as a Viral Inactivator

Viruses, Vol. 11, Pages 1064: Erythromycin Estolate Inhibits Zika Virus Infection by Blocking Viral Entry as a Viral Inactivator Viruses doi: 10.3390/v11111064 Authors: Xiaohuan Wang Shuai Xia Peng Zou Lu Lu Recently, Zika virus (ZIKV) has attracted much attention in consideration of its association with severe neurological complications including fetal microcephaly. However, there are currently no prophylactic vaccines or therapeutic drugs approved for clinical treatments of ZIKV infection. To determine the potential anti-ZIKV inhibitors, we screened a library of clinical drugs with good safety profiles. Erythromycin estolate (Ery-Est), one of the macrolide antibiotics, was found to effectively inhibit ZIKV infection in different cell types and significantly protect A129 mice from ZIKV-associated neurological signs and mortality. Through further investigation, Ery-Est was verified to inhibit ZIKV entry by disrupting the integrity of the viral membrane which resulted in the loss of ZIKV infectivity. Furthermore, Ery-Est also showed inhibitory activity against dengue virus (DENV) and yellow fever virus (YFV). Thus, Ery-Est may be a promising drug for patients with ZIKV infection, particularly pregnant women.
Source: Viruses - Category: Virology Authors: Tags: Article Source Type: research

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In this study, we evaluated ATA as a potential antiviral drug against ZIKV replication. The antiviral activity of ATA against ZIKV replication in vitro showed median inhibitory concentrations (IC50) of 13.87 ± 1.09 μM and 33.33 ± 1.13 μM in Vero and A549 cells, respectively; without showing any cytotoxic effect in both cell lines (median cytotoxic concentration (CC50)> 1,000 μM). Moreover, ATA protected both cell types from ZIKV-induced cytopathic effect (CPE) and apoptosis in a time- and concentration-dependent manner. In addition, pre-treatment of Vero cells with ATA for up to 72 h also resulted...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Conclusions Apart West Nile virus (78), thousands of congenital microcephaly cases, fetal brain tissue damage and neurological syndromes have been associated with ZIKV infection. Unfortunately, the epidemics of this mosquito born, and a relative stable virus is on a rise. Although congenital microcephaly is a rare disorder however, due to lack of standardized diagnostic test facilities, the incidence in the geographically widespread ZIKV epidemic regions is higher. Animals studies showed that ZIKV is a neurotropic virus. It directly targets the developing embryonic brain cells by inducing apoptosis, cell-cycle arrest, and...
Source: Frontiers in Neurology - Category: Neurology Source Type: research
LITFL • Life in the Fast Lane Medical Blog LITFL • Life in the Fast Lane Medical Blog - Emergency medicine and critical care medical education blog aka Tropical Travel Trouble 010 Peer Reviewer: Dr Jennifer Ho, ID physician QLD, Australia You are an ED doc working in Perth over schoolies week. An 18 yo man comes into ED complaining of fever, rash a “cracking headache” and body aches. He has just hopped off the plane from Bali where he spent the last 2 weeks partying, boozing and running amok. He got bitten by “loads” of mosquitoes because he forgot to take insect repellent. On e...
Source: Life in the Fast Lane - Category: Emergency Medicine Authors: Tags: Clinical Cases Tropical Medicine arthralgia dengue fever rash Source Type: blogs
Not long ago, Zika virus was dominating headlines. A new infection was hardly ever heard about before then, yet is now affecting hundreds of thousands of people in Latin America, causing disfiguration and microcephalia in new-born babies. Microcephalia is caused by severe delayed and abnormal development of the brain, resulting in the range of intellectual disability, dwarfism, poor motor functions and speech. With no cure or even preventive vaccination available, many women in the most affected regions were reportedly considering postponing any planned pregnancies. The virus was actually discovered back in 1947 in Zika fo...
Source: World of Psychology - Category: Psychiatry & Psychology Authors: Tags: Brain and Behavior Brain Blogger Health-related Publishers Research brain cancer Delivery glioblastoma Immune System microcephalia Pregnancy Sexual Contact stem cells Zika virus Source Type: blogs
Conclusions and Relevance: Mosquito-borne disease should be considered in pregnant women who present with a febrile illness and a relevant exposure history. Prompt recognition can allow supportive treatment to the mother and fetal resuscitation and surveillance. Target Audience: Obstetricians and gynecologists, family physicians. Learning Objectives: After completing this activity, the learner should be better able to describe the presentation, symptoms, and diagnosis of mosquito-borne diseases including malaria, chikungunya, dengue, yellow fever, West Nile virus, Zika, and filariasis; describe the risks of mosquito-born...
Source: Obstetrical and Gynecological Survey - Category: OBGYN Tags: CME Articles Source Type: research
Funding Opportunity ID: 292882 Opportunity Number: RFA-FD-17-002 Opportunity Title: Identification of Target Peptide Sequences for Zika IgM Diagnostic DeviceOpportunity Category: DiscretionaryOpportunity Category Explanation: Funding Instrument Type: Cooperative AgreementCategory of Funding Activity: AgricultureConsumer ProtectionFood and NutritionCategory Explanation: CFDA Number(s): 93.103Eligible Applicants: Others (see text field entitled "Additional Information on Eligibility" for clarification)Additional Information on Eligibility: The following organization is eligible to apply: PEPperPrint GmbHAge...
Source: Grants.gov - Category: Research Tags: Agriculture Consumer Protection Food and Nutrition Source Type: funding
Conclusions To our knowledge, the developed RT-RPA assay is the first sensitive rapid molecular assay applied on field samples for the detection of ZIKV in 15 minutes, which could be implemented at the point of need in a mobile suitcase laboratory 5 to make testing of pregnant women available directly in rural settings. Moreover, combining it with the developed dengue 6, chikungunya 7 and Sigma 8 RT-RPA assays will allow its use during outbreak investigations. Sigma virus infects exclusively dipterans, which is a good candidate to be used in the future as an extraction and RPA reaction control. Data Availability Statement...
Source: PLOS Currents Outbreaks - Category: Epidemiology Authors: Source Type: research
Serological diagnosis of Zika virus (ZIKV) infections is challenging due to high cross-reactivity between flaviviruses. We evaluated the diagnostic performance of a novel anti-ZIKV ELISA based on recombinant ZIKV non-structural protein 1 (NS1). Assay sensitivity was examined using sera from 27 patients with reverse transcription (RT)-PCR-confirmed and 85 with suspected ZIKV infection. Specificity was analysed using sera from 1,015 healthy individuals. Samples from 252 patients with dengue virus (n?=?93), West Nile virus (n?=?34), Japanese encephalitis virus (n?=?25), chikungunya virus (n?=?19) orPlasmodium spp. (n?=?69) in...
Source: Eurosurveillance - Category: Infectious Diseases Source Type: research
Abstract Zika virus is a single-stranded RNA virus in the genus Flavivirus and is closely related to dengue, West Nile, Japanese encephalitis, and yellow fever viruses (1,2). Among flaviviruses, Zika and dengue virus share similar symptoms of infection, transmission cycles, and geographic distribution. Diagnostic testing for Zika virus infection can be accomplished using both molecular and serologic methods. For persons with suspected Zika virus disease, a positive real-time reverse transcription-polymerase chain reaction (rRT-PCR) result confirms Zika virus infection, but a negative rRT-PCR result does not exclud...
Source: MMWR Morb Mortal Wkl... - Category: Epidemiology Authors: Tags: MMWR Morb Mortal Wkly Rep Source Type: research
Rio de Janeiro is expecting about 500,000 visitors for the Olympic and Paralympic games this August. If you’re one of them, there are a few things you need to consider in order to have a safe, happy and healthy trip to Brazil this year.   1. Make sure you’re up to date on all your vaccines. This is travel safety 101. Infectious disease loves a crowd, and one way to make sure a nasty bug doesn't hitch a ride with you is to get vaccinated.  What to do:  Make an appointment with a travel doctor now to make sure you’re current on all your regular vaccines (measles, mumps, rubella, etc.) an...
Source: Science - The Huffington Post - Category: Science Source Type: news
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