Bacterial Microcompartment-Dependent 1,2-Propanediol Utilization Stimulates Anaerobic Growth of Listeria monocytogenes EGDe

Bacterial microcompartments (BMCs) are proteinaceous organelles that optimize specific metabolic pathways referred to as metabolosomes involving transient production of toxic volatile metabolites such as aldehydes. Previous bioinformatics analysis predicted the presence of BMCs in 23 bacterial phyla including foodborne pathogens and a link with gene clusters for the utilization of host-derived substrates such as 1,2-propanediol utilization, i.e., the Pdu cluster. Although, transcriptional regulation of the Pdu cluster and its role in Listeria monocytogenes virulence in animal models have recently been reported, the experimental identification and the physiological role of BMCs in L. monocytogenes is still unexplored. Here, we ask whether BMCs could enable utilization of 1,2-propanediol (Pd) in L. monocytogenes under anaerobic conditions. Using L. monocytogenes EGDe as a model strain, we could demonstrate efficient utilization of Pd with concomitant production of 1-propanol and propionate after 24 h of anaerobic growth, while the utilization was significantly reduced in aerobic conditions. In line with this, expression of genes encoding predicted shell proteins and the signature enzyme propanediol dehydratase is upregulated more than 20-fold in cells anaerobically grown in Pdu-induced versus non-induced control conditions. Additional proteomics analysis confirmed the presence of BMC shell proteins and Pdu enzymes in cells that show active degradation of Pd. Furthermore, using ...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research

Related Links:

White Castle has initiated a voluntary recall of a limited number of frozen 6 pack cheeseburgers, frozen 6 pack hamburgers, frozen 6 pack jalapeno cheeseburgers, and 16 pack hamburgers, 16 pack cheeseburgers for the possible presence of Listeria monocytogenes.
Source: FDA Food Safety - Category: Food Science Authors: Source Type: alerts
They may take our broccoli florets, our chicken, and our sausage products, but they will never take our sushi. Well, actually they can.
Source: Forbes.com Healthcare News - Category: Pharmaceuticals Authors: Source Type: news
They may take our broccoli florets, our chicken, and our sausage products, but they will never take our sushi. Well, actually they can.
Source: Forbes.com Healthcare News - Category: Pharmaceuticals Authors: Source Type: news
CONCLUSION: Infections caused by L. monocytogenes were uncommon but could cause severe sepsis and mortality, especially in susceptible populations. Our study focused on neurological involvement and severe invasive forms of listeriosis. Neuroinvasive forms were the most important risk factors for severe illness but not for mortality. PMID: 31791803 [PubMed - as supplied by publisher]
Source: Medicina Clinica - Category: General Medicine Tags: Med Clin (Barc) Source Type: research
Products are sold in 31 states and Washington, D.C., by Trader Joe's, 7-Eleven, Walgreens and other retailers.
Source: Health News: CBSNews.com - Category: Consumer Health News Source Type: news
Publication date: Available online 3 December 2019Source: LWTAuthor(s): Jin-Young Han, Won-Jae Song, Joo Hyun Kang, Sea C. Min, Sangheum Eom, Eun Jeong Hong, Seungmin Ryu, Seongbong Kim, Sangwoo Cho, Dong-Hyun KangAbstractWe evaluated the effect of plasma-activated water (PAW) on the populations of foodborne pathogens and food-spoilage microorganisms on Korean rice cake. PAW was produced by treating distilled water with two atmospheric dielectric-barrier discharges (51.7 W, 14.4 kHz, air discharge) for 20 min. The inactivation effect of PAW on indigenous microorganism and inoculated Escherichia coli O157:H7, Salmonel...
Source: LWT Food Science and Technology - Category: Food Science Source Type: research
Publication date: Available online 3 December 2019Source: Food Research InternationalAuthor(s): Elvira Frías, Yenea Iglesias, Avelino Alvarez-Ordóñez, Miguel Prieto, Montserrat González-Raurich, Mercedes LópezAbstractThe efficacy of Cold Atmospheric Pressure Plasma (CAPP) for the inactivation of Salmonella enterica serovar Enteritidis, Salmonella enterica serovar Typhimurium, Listeria monocytogenes and Escherichia coli O157:H7 on tofu was evaluated. The potential of using Plasma Activated Water (PAW) as an immersion solution for controlling microbial growth in tofu throughout its shelf-li...
Source: Food Research International - Category: Food Science Source Type: research
Products are sold in 31 states and Washington, D.C., by Trader Joe's, 7-Eleven, Walgreens and other retailers.
Source: Health News: CBSNews.com - Category: Consumer Health News Source Type: news
Ready-to-eat sushi, salads and spring rolls are being recalled voluntarily over fears of listeria contamination. The Fuji Food products are sold in 31 states and Washington, D.C. at retailers including Trader Joe's, 7-Eleven and Walgreens. The products are packed in plastic, with most having sell-by dates of Nov. 22 to Dec. 6.
Source: Health News: CBSNews.com - Category: Consumer Health News Source Type: news
BOSTON (CBS/CNN) — Trader Joe’s has announced a voluntary recall of several of its products that may have been contaminated with listeria. So far there are no reports of anyone getting sick, but the company said it is taking the measures as a precaution. Each of the products are packaged in plastic trays with clear lids. The Trader Joe’s brand food items listed in the recall include ready-to-eat meals of California Rolls, Classic California Rolls with Brown Rice &Avocado, Spicy California Rolls, Tempura Shrimp Crunch Rolls, Tofu Spring Rolls, Shrimp Spring Rolls, Smoked Salmon Philly Roll, Smoked Salm...
Source: WBZ-TV - Breaking News, Weather and Sports for Boston, Worcester and New Hampshire - Category: Consumer Health News Authors: Tags: Boston News Consumer Featured Health Syndicated CBSN Boston CNN Listeria Recall Trader Joe's Source Type: news
More News: Genetics | Listeria | Microbiology | Study | Toxicology