Botrydial confers Botrytis cinerea the ability to antagonize soil and phyllospheric bacteria

Publication date: Available online 15 November 2019Source: Fungal BiologyAuthor(s): Paulina Vignatti, María E. Gonzalez, Edgardo C. Jofré, Hernando J. Bolívar-Anillo, Javier Moraga, Muriel Viaud, Isidro G. Collado, Fernando L. PieckenstainAbstractThe role of the sesquiterpene botrydial in the interaction of the phytopathogenic fungus Botrytis cinerea and plant-associated bacteria was analyzed. From a collection of soil and phyllospheric bacteria, nine strains sensitive to growth-inhibition by B. cinerea were identified. B. cinerea mutants unable to produce botrydial caused no bacterial inhibition, thus demonstrating the inhibitory role of botrydial. A taxonomic analysis showed that these bacteria corresponded to different Bacillus species (six strains), Pseudomonas yamanorum (two strains) and Erwinia aphidicola (one strain). Inoculation of WT and botrydial non-producing mutants of B. cinerea along with B. amyloliquefaciens strain MEP218 in soil demonstrated that both microorganisms exert reciprocal inhibitory effects; the inhibition caused by B. cinerea being dependent on botrydial production. Moreover, botrydial production was modulated by the presence of B. amyloliquefaciens MEP218 in confrontation assays in vitro. Purified botrydial in turn, inhibited growth of Bacillus strains in vitro and cyclic lipopeptide (surfactin) production by B. amyloliquefaciens MEP218. As a whole, results demonstrate that botrydial confers B. cinerea the ability to inhibit potential biocontro...

Source: Fungal Biology - November 15, 2019 Category: Biology Source Type: research