Improvement of a recombinant avian avulavirus serotype 10 vectored vaccine by the addition of untranslated regions.

Improvement of a recombinant avian avulavirus serotype 10 vectored vaccine by the addition of untranslated regions. Vaccine. 2019 Nov 09;: Authors: Tsunekuni R, Tanikawa T, Nakaya T, Saito T Abstract BACKGROUND: We have previously developed a recombinant avian avulavirus serotype 10 (rAAvV-10/HA) expressing the hemagglutinin (HA) gene of a highly pathogenic avian influenza virus (HPAIV) as an emergency vaccine for poultry. rAAvV-10/HA can overcome the activity of the anti-AAvV-1 (Newcastle disease virus) antibody acquired by commercial chickens upon routine vaccination. Most chickens do not have the anti-AAvV-10 antibody, which could interfere with the vaccine efficacy. However, the vaccine efficacy of rAAvV-10/HA is not satisfactory in chickens even though it affords protection against an HPAIV challenge. In the present study, we improved the rAAvV-10/HA vaccine by enhancing the expression of the exogenous HA protein. METHODS: The 5' and 3' untranslated regions (UTR) of each AAvV-10 gene were flanked with the exogenous HA gene cassette to modify rAAvV-10/HA, yielding different rAAv10-UTRs. As a control, rAAv10-nonUTR that did not contain any UTRs was generated. The effects of UTRs on mRNA transcription, HA protein expression, and vaccine efficacy were then examined using embryonated chicken eggs and white leghorn chickens. RESULTS: The proportion of the HA gene mRNA among the vector-derived mRNAs (1.55-1.84-fold incr...
Source: Vaccine - Category: Allergy & Immunology Authors: Tags: Vaccine Source Type: research