Improvement of a recombinant avian avulavirus serotype 10 vectored vaccine by the addition of untranslated regions.

Improvement of a recombinant avian avulavirus serotype 10 vectored vaccine by the addition of untranslated regions. Vaccine. 2019 Nov 09;: Authors: Tsunekuni R, Tanikawa T, Nakaya T, Saito T Abstract BACKGROUND: We have previously developed a recombinant avian avulavirus serotype 10 (rAAvV-10/HA) expressing the hemagglutinin (HA) gene of a highly pathogenic avian influenza virus (HPAIV) as an emergency vaccine for poultry. rAAvV-10/HA can overcome the activity of the anti-AAvV-1 (Newcastle disease virus) antibody acquired by commercial chickens upon routine vaccination. Most chickens do not have the anti-AAvV-10 antibody, which could interfere with the vaccine efficacy. However, the vaccine efficacy of rAAvV-10/HA is not satisfactory in chickens even though it affords protection against an HPAIV challenge. In the present study, we improved the rAAvV-10/HA vaccine by enhancing the expression of the exogenous HA protein. METHODS: The 5' and 3' untranslated regions (UTR) of each AAvV-10 gene were flanked with the exogenous HA gene cassette to modify rAAvV-10/HA, yielding different rAAv10-UTRs. As a control, rAAv10-nonUTR that did not contain any UTRs was generated. The effects of UTRs on mRNA transcription, HA protein expression, and vaccine efficacy were then examined using embryonated chicken eggs and white leghorn chickens. RESULTS: The proportion of the HA gene mRNA among the vector-derived mRNAs (1.55-1.84-fold increase vs. the control) and H...
Source: Vaccine - Category: Allergy & Immunology Authors: Tags: Vaccine Source Type: research

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“Everyone knows that pestilences have a way of recurring in the world,” observes Albert Camus in his novel The Plague. “Yet somehow we find it hard to believe in ones that crash down on our heads from a blue sky. There have been as many plagues as wars in history; yet plagues and wars always take people by surprise.” Camus was imagining a fictional outbreak of plague in 1948 in Oran, a port city in northwest Algeria. But at a time when the world is reeling from a very real microbial emergency sparked by the emergence of a novel coronavirus in Wuhan, central China, his observations are as pertinent a...
Source: TIME: Health - Category: Consumer Health News Authors: Tags: Uncategorized 2019-nCoV health ideas Source Type: news
Source: National Institutes of Health (NIH). Published: 1/8/2019. This one-hour, 48-minute presentation discusses the history of pandemics, their challenges, and the next influenza pandemic. Topics include the role of viral, host, and secondary bacterial factors in influenza pathogenesis; contemporary Avian influenza, and why an HIV vaccine Is essential for ending the HIV/AIDS pandemic. (Video or Multimedia)
Source: Disaster Lit: Resource Guide for Disaster Medicine and Public Health - Category: International Medicine & Public Health Source Type: news
Source: National Institutes of Health (NIH). Published: 1/8/2019. This one-hour, 48-minute presentation discusses the history of pandemics, their challenges, and the next influenza pandemic. Topics include the role of viral, host, and secondary bacterial factors in influenza pathogenesis; contemporary Avian influenza, and why an HIV vaccine Is essential for ending the HIV/AIDS pandemic. (Video or Multimedia)
Source: Disaster Lit: Resource Guide for Disaster Medicine and Public Health - Category: International Medicine & Public Health Source Type: news
Publication date: September 2018Source: Veterinary Microbiology, Volume 223Author(s): Yixue Sun, Jing Qian, Xiaohong Xu, Yubo Tang, Wenzhang Xu, Wentao Yang, Yanlong Jiang, Guilian Yang, Zhuang Ding, Yanlong Cong, Chunfeng WangAbstractH9N2 avian influenza viruses are of significance in poultry and public health for the past two decades. Vaccination plays an important role in preventing the infection in domestic poultry. Current H9N2 vaccines have not yet offered ideal protection and eliminated shedding of G57 genotype viruses responsible for H9N2 outbreaks during 2010–2013. Targeted vaccination is a promising strateg...
Source: Veterinary Microbiology - Category: Veterinary Research Source Type: research
Publication date: Available online 17 July 2018Source: Veterinary MicrobiologyAuthor(s): Yixue Sun, Jing Qian, Xiaohong Xu, Yubo Tang, Wenzhang Xu, Wentao Yang, Yanlong Jiang, Guilian Yang, Zhuang Ding, Yanlong Cong, Chunfeng WangAbstractH9N2 avian influenza viruses are of significance in poultry and public health for the past two decades. Vaccination plays an important role in preventing the infection in domestic poultry. Current H9N2 vaccines have not yet offered ideal protection and eliminated shedding of G57 genotype viruses responsible for H9N2 outbreaks during 2010-2013. Targeted vaccination is a promising strategy t...
Source: Veterinary Microbiology - Category: Veterinary Research Source Type: research
Across China, the virus that could spark the next pandemic is already circulating. It’s a bird flu called H7N9, and true to its name, it mostly infects poultry. Lately, however, it’s started jumping from chickens to humans more readily–bad news, because the virus is a killer. During a recent spike, 88% of people infected got pneumonia, three-quarters ended up in intensive care with severe respiratory problems, and 41% died. What H7N9 can’t do–yet–is spread easily from person to person, but experts know that could change. The longer the virus spends in humans, the better the chance that i...
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Conclusions Using a systematic evidence base and consensus development process among stakeholders within and outside VA, we report on the first national VA comprehensive emergency management program evaluation and research agenda. VA provides a unique national laboratory for the conduct of high quality research that will improve VA’s and our Nation’s emergency medical and public health preparedness and the role of health delivery systems in that endeavor. To effectively foster the conduct and expansion of emergency management evaluation and research within VA, the consensus was that VA needs to build program ev...
Source: PLOS Currents Disasters - Category: Global & Universal Authors: Source Type: research
Editor’s Note: This post reflects on a speech on pandemic preparedness Dr. Fauci gave on January 10, 2017 in Washington, DC, hosted by  The Center for Global Health Science and Security at Georgetown University Medical Center, the Harvard Global Health Institute, and Health Affairs. One of the most important challenges facing the new Administration is preparedness for the pandemic outbreak of an infectious disease. Infectious diseases will continue to pose a significant threat to public health and the economies of countries worldwide. The U.S. government will need to continue its investment to combat these ...
Source: Health Affairs Blog - Category: Health Management Authors: Tags: Featured Global Health Policy Ebola HIV/AIDS NIH pandemic preparedness Zika Source Type: blogs
This virus-like particle (VLP) vaccine technology for influenza viruses, based on a mixture of VLPs expressing the hemagglutinin protein or the neuraminidase protein from influenza virus strains belonging to different virus subtypes, has demonstrated broad protection against lethal challenge in mice with various influenza virus strains and virus subtypes. Results from ferret and mouse studies demonstrate broad heterosubtypic protection against various influenza virus subtypes further supporting and strengthening the proposed application of this technology as a universal influenza virus vaccine.IC: NIAIDNIH Ref. No.:&n...
Source: NIH OTT Licensing Opportunities - Category: Research Authors: Source Type: research
Funding Opportunity Number: PA-13-179 Opportunity Category: DiscretionaryFunding Instrument Type: GrantCategory of Funding Activity: HealthCFDA Number: 93.85593.856Eligible Applicants State governmentsCounty governmentsCity or township governmentsSpecial district governmentsIndependent school districtsPublic and State controlled institutions of higher educationNative American tribal governments (Federally recognized)Public housing authorities/Indian housing authoritiesNative American tribal organizations (other than Federally recognized tribal governments)Nonprofits having a 501(c)(3) status with the IRS, other t...
Source: Grants.gov - Category: Research Tags: Health Source Type: funding
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