Stanniocalcin-1 ameliorates cerebral ischemia by decrease oxidative stress and blood brain barrier permeability

Publication date: Available online 13 November 2019Source: Microvascular ResearchAuthor(s): Sandra Bonfante, Amanda Della Giustina, Lucineia Gainski Danielski, Tais Denicol, Larissa Joaquim, Erica Biehl, Gabriel Scopel, Raquel Jaconi de Carli, Marcos Hubner, Taise Cardoso, Talita Tuon, Jaqueline Generoso, Tatiana Barichello, Silvia Terra, Fabricia PetronilhoAbstractBlood brain barrier (BBB) permeability and oxidative stress have been reported to be important mechanisms for brain damage following ischemic stroke and stanniocalcin-1 (STC-1), a neuroprotective protein, has anti-inflammatory and anti-oxidative stress properties. Herein, we report the effect of STC-1 on BBB permeability and brain oxidative stress after stroke in an animal model. Male Wistar received an intracerebroventricularly injection of human recombinant STC-1 (100 ng/kg) or saline and were subjected to sham procedure or global cerebral ischemia/reperfusion (I/R) model. Six and 24 h after I/R, neurological evaluation was performed; at 24 h brain water content was evaluated in the total brain, and BBB permeability, nitrite/nitrate (N/N) concentration, lipid peroxidation, protein carbonyls formation, superoxide dismutase (SOD) and catalase (CAT) activity were determined in the hippocampus, cortex, prefrontal cortex, striatum and cerebellum. Rats exhibited neurological deficit at 6 and 24 h after I/R and STC-1 reduction at 24 h. After I/R there were an increase of brain water content, BBB permeability i...
Source: Microvascular Research - Category: Biochemistry Source Type: research