Bioluminescence Imaging to Study Mature Biofilm Formation by Candida spp. and Antifungal Activity In Vitro and In Vivo.

Bioluminescence Imaging to Study Mature Biofilm Formation by Candida spp. and Antifungal Activity In Vitro and In Vivo. Methods Mol Biol. 2020;2081:127-143 Authors: Van Dyck K, Van Dijck P, Vande Velde G Abstract The widespread use of indwelling medical devices has increased the number of device-related infections in hospitalized patients. These infections are often associated with the formation of biofilms on the medical implants that are difficult to treat because of their resistance to the classical antifungal drugs. The most common fungi isolated from catheters and other medical devices are Candida species. The Candida genus contains multiple species of which C. albicans and C. glabrata are the two most common pathogenic yeasts in humans. A limited number of animal models is available for investigating host-pathogen interactions and testing novel antifungal drugs in vivo against these species. Fungal load in biofilms in these models is traditionally analyzed postmortem, requiring host sacrifice and enumeration of microorganisms from individual biofilms in order to evaluate the amount of colony forming units and the efficacy of antifungal treatment. Bioluminescence imaging (BLI) made compatible with small animal models for in vivo biofilm formation is a valuable tool to follow biofilm development and its treatment longitudinally. Due to the noninvasive nature of BLI, the imaging procedure can be repeated in the same animal, allowing for follow-up of t...
Source: Mol Biol Cell - Category: Molecular Biology Authors: Tags: Methods Mol Biol Source Type: research

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Source: Biomedizinische Technik/Biomedical Engineering - Category: Biomedical Engineering Tags: Biomed Tech (Berl) Source Type: research
In conclusion, the results suggest that, because of its bioactivity including the antioxidant, antimicrobial, and enzyme inhibition properties, the D. macrolepis can be accepted as a promising and natural source for the industrial applications. The present study is the first study, in which the bioactive components and the antioxidant, antimicrobial, and enzyme inhibition properties of endemic D. macrolepis plant were determined.
Source: Saudi Pharmaceutical Journal - Category: Drugs & Pharmacology Source Type: research
o-Cadavid E Abstract The biotechnological potential of yeasts associated to different habitats in Colombia has been poorly studied, especially the yeasts associated with different plant structures. Fruit pulps are interesting substrates mainly for the growth of yeast species, that can positively affect the productivity and quality of some bioeconomic species. Therefore, the objective of this study was to identify the dominant yeast species associated with mango and rose apple fruit pulps in Cali, Colombia. A total of 90 isolates were obtained, which were grouped considering their colony morphology. The D1/D2 domai...
Source: An Acad Bras Cienc - Category: Science Authors: Tags: An Acad Bras Cienc Source Type: research
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Source: Food Research International - Category: Food Science Source Type: research
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Source: Comparative Biochemistry and Physiology Part C: Toxicology and Pharmacology - Category: Toxicology Source Type: research
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Source: Proteomics - Category: Biochemistry Authors: Tags: Research Article Source Type: research
Conclusions: Optimal efficacy of amphotericin B against C. albicans is achieved in cold storage conditions at concentrations ≥1.25 μg/mL, and 2.5 μg/mL reduces Candida contamination by>90% after 6 hours of cold storage without sacrificing CEC health.
Source: Cornea - Category: Opthalmology Tags: Basic Investigation Source Type: research
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Source: The Journal of Prosthetic Dentistry - Category: Dentistry Source Type: research
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Source: Materials Science and Engineering: C - Category: Materials Science Source Type: research
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