Assessment of Immune Response Following Dendritic Cell-Based Immunotherapy in Pediatric Patients With Relapsing Sarcoma

Monocyte-derived dendritic cell (DC)-based vaccines loaded with tumor self-antigens represent a novel approach in anticancer therapy. We evaluated DC-based anticancer immunotherapy (ITx) in an academic Phase I/II clinical trial for children, adolescent, and young adults with progressive, recurrent, or primarily metastatic high-risk tumors. The primary endpoint was safety of intradermal administration of manufactured DCs. Here, we focused on relapsing high-risk sarcoma subgroup representing a major diagnosis in DC clinical trial. As a part of peripheral blood immunomonitoring, we evaluated quantitative association between basic cell-based immune parameters. Furthermore, we describe the pattern of these parameters and their time-dependent variations during the DC vaccination in the peripheral blood immunograms. The peripheral blood immunograms revealed distinct patterns in particular patients in the study group. As a functional testing, we evaluated immune response of patient T-cells to the tumor antigens presented by DCs in the autoMLR proliferation assay. This analysis was performed with T-cells obtained prior to DC ITx initiation and with T-cells collected after the fifth dose of DCs, demonstrating that the anticancer DC-based vaccine stimulates a preexisting immune response against self-tumor antigens. Finally, we present clinical and immunological findings in a Ewing's sarcoma patient with an interesting clinical course. Prior to DC therapy, we observed prevailing CD8+ T-c...
Source: Frontiers in Oncology - Category: Cancer & Oncology Source Type: research

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Conclusion: Our findings highlight the highly expression of SR-B1 in liposarcomas. SR-B1 may be a potential biomarker for the diagnosis of liposarcoma and may indicate the degree of differentiation of liposarcomas.
Source: Journal of Cancer - Category: Cancer & Oncology Authors: Tags: Research Paper Source Type: research
Authors: Segal O, Kollander Y, Gosheger G PMID: 31814350 [PubMed - in process]
Source: The Israel Medical Association Journal - Category: General Medicine Tags: Isr Med Assoc J Source Type: research
Publication date: March 2020Source: Human Pathology: Case Reports, Volume 19Author(s): Mia C. Weiss, Marta Batus, Rahul B. Jadhav, Manuel Utset, Cristina O'Donoghue, Ira MillerAbstractHere we present a case of a 56 year old male with an Ewing-like bilateral retroperitoneal sarcoma with evidence of a EWSR1-POU5F1 fusion gene. Biopsy showed a high grade round cell sarcoma expressing strong membranous CD99 antigen. The tumor shrank considerably after extensive neoadjuvant chemo-radiation therapy. The resected specimens showed complete regression of the malignant component, with the residual tumor resembling ganglioglioma as...
Source: Human Pathology: Case Reports - Category: Pathology Source Type: research
Authors: Deng Y, Xie Q, Zhang G, Li S, Wu Z, Ma Z, He X, Gao Y, Wang Y, Kang X, Wang J Abstract Ewing's sarcoma (ES) is a common malignant bone tumor in children and adolescents. Although great efforts have been made to understand the pathogenesis and development of ES, the underlying molecular mechanism remains unclear. The present study aimed to identify new key genes as potential biomarkers for the diagnosis, targeted therapy or prognosis of ES. mRNA expression profile chip data sets GSE17674, GSE17679 and GSE45544 were downloaded from the Gene Expression Omnibus database. Differentially expressed genes (DEGs) w...
Source: Oncology Letters - Category: Cancer & Oncology Tags: Oncol Lett Source Type: research
We present a case of a 19-year-old woman with Ewing sarcoma of the iliac bone in whom 68Ga-PSMA-HBED-CC PET/CT showed high radiotracer activity in the primary tumor. The present case documents the in vivo expression of PSMA in Ewing sarcoma family of tumors and adds on to the list of nonprostatic malignancies showing PSMA expression.
Source: Clinical Nuclear Medicine - Category: Nuclear Medicine Tags: Interesting Images Source Type: research
We describe the clinical, pathological and genetic features in a 42 year old male with a 3 month history of arm weakness. MRI of the cervical spine with and without contrast revealed an intradural, extra-axial enhancing lesion involving the spinal canal from the foramen magnum to the level of C4. Partial surgical resection revealed a malignant neuroepithelial tumor. Genetic analysis identified a EWSRA-FLI1 (exon 7-exon 6) fusion, transcript suggesting classification as a Ewing's sarcoma/primitive neuroectodermal tumor. The case highlights the variability in the presentation of ES and value of genetic testing in a...
Source: Interdisciplinary Neurosurgery - Category: Neurosurgery Source Type: research
In conclusion, the histopathological presence of Homer-Wright rosettes and immunohistochemical markers such as CD99, FLI-1 and CK are valuable factors for the diagnosis of ES, although cytogenetic analysis is considered the gold standard. Complete surgery is the most effective treatment option for ES treatment. Adjuvant radiotherapy and combination chemotherapy can also improve the survival rate of patients postoperatively. PMID: 31788090 [PubMed]
Source: Oncology Letters - Category: Cancer & Oncology Tags: Oncol Lett Source Type: research
In conclusion, the DEGs, associated pathways and hub genes identified in the present study help elucidate the underlying molecular mechanisms of ES carcinogenesis and progression, and provide potential molecular targets and biomarkers for ES. PMID: 31788075 [PubMed]
Source: Oncology Letters - Category: Cancer & Oncology Tags: Oncol Lett Source Type: research
AbstractEwing sarcoma (ES) and Ewing-like sarcomas are highly aggressive round cell mesenchymal neoplasms, most often occurring in children and young adults. The identification of novel molecular alterations has greatly contributed to a profound reappraisal of classification, to the extent that the category of undifferentiated round cell sarcoma has significantly shrunk. In fact, in addition to Ewing sarcoma, we currently recognize three main categories: round cell sarcomas withEWSR1 gene fusion with non-ETS family members,CIC-rearranged sarcomas, andBCOR-rearranged sarcomas. Interestingly, despite significant morphologic ...
Source: Virchows Archiv - Category: Pathology Source Type: research
Conditions:   Osteosarcoma Metastatic;   Ewing's Sarcoma Metastatic Intervention:   Drug: Chemotherapy (gemcitabine&docetaxel) plus BIO-11006 Sponsor:   Nicklaus Children's Hospital f/k/a Miami Children's Hospital Recruiting
Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials
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