Physicochemical and functional characterization of mannan exopolysaccharide from Weissella confusa MD1 with bioactivities.

Physicochemical and functional characterization of mannan exopolysaccharide from Weissella confusa MD1 with bioactivities. Int J Biol Macromol. 2019 Nov 09;: Authors: Kant Lakra A, Domdi L, Mohd Tilwani Y, Arul V Abstract The present study focused on production, optimization and characterization of exopolysaccharide (EPS) from Weissella confusa MD1. The purified EPS MD1 was also evaluated for in vitro biological activities. The maximum yield of EPS (10.07 ± 0.32 g/L) was obtained with optimized culture conditions of 35 ˚C at pH 6.5 for 36 h with 4% (w/v) galactose and 1% (w/v) ammonium nitrate supplemented in MRS broth. The crude EPS was purified with diethylamino ethanol-Sepharose Fast Flow column and Sephadex-G 75 column. The physicochemical and functional characterization of the EPS was done by high-performance thin-layer chromatography (HPTLC), high-performance gel permeation chromatography (HPGPC), fourier transform infrared (FTIR) spectroscopy, nuclear magnetic resonance (NMR), thermo gravimetric and differential scanning calorimetric (TG-DSC) analysis, x-ray diffraction (XRD) and scanning electron microscopy (SEM). HPGPC analysis showed molecular weight of purified EPS MD1 as 2.909 KDa. Monosaccharide analysis showed that EPS was a novel mannan with mannose as the only monomeric unit present, suggesting EPS to be a homopolysaccharide containing →6) α-Man p (1→ linkages. Microstructure studies by SEM showed MD1 EPS has globu...
Source: International Journal of Biological Macromolecules - Category: Biochemistry Authors: Tags: Int J Biol Macromol Source Type: research

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CONCLUSIONS: In vitro antimicrobial activity showed that the suspension of ZNPs with 250 ppm concentration could effectively cause a proper reduction and inhibition in the growth of both Gram-positive and Gram-negative microorganisms. Therefore, the results revealed the efficacy of the antibacterial properties of synthesized ZNPs suspension in aqueous media. PMID: 31847595 [PubMed - as supplied by publisher]
Source: Current Eye Research - Category: Opthalmology Authors: Tags: Curr Eye Res Source Type: research
Publication date: Available online 13 December 2019Source: Chinese Chemical LettersAuthor(s): Li Jin, Xun Liu, Changhao Bian, Jie Sheng, Yishan Song, Yongheng ZhuAbstractTo develop a novel food preservation technology for efficiently enhance bactericidal activity in a long term, hollow mesoporous silica spheres (HMSS) with regular nanostructures were applied to encapsulate natural organic antimicrobial agents. The chemical structures, morphologies and thermal stabilities of linalool, HMSS and linalool-functionalized hollow mesoporous silica spheres (L-HMSS) nanoparticles were evaluated by polarimeter, field emission scanni...
Source: Chinese Chemical Letters - Category: Chemistry Source Type: research
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Source: Applied Microbiology and Biotechnology - Category: Microbiology Authors: Tags: Appl Microbiol Biotechnol Source Type: research
Conclusion This study confirms the in vitro antibacterial activity of BIOCITRO® against Gram-negative and Gram-positive bacteria. For most of the strains, the product reached the bactericidal effect at the same concentration of the bacteriostatic effect and maximum difference between MIC and MBC was two dilution steps. The less susceptible species of the study were S. enterica ssp. enterica and E. coli with MBC90 values of 256 and 128 μg/mL, respectively, while the most susceptible was C. perfringens with MBC90 of 16 μg/mL. After short exposition time to the product, the significant effect over the viability of ...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Conclusion Genomic analyses herein performed allowed us to confirm the recently (Pérez-Cataluña et al., 2018a,b) suggested amendment of A. butzleri as Al. butzlerii, comb. nov. Antimicrobial susceptibility tests defined Ab 55 and Ab 6V strains as resistant to vancomycin, tetracyclin, nalidixic acid (only Ab 55 whereas Ab 6V is intermediate resistant), erythromycin, and β-lactam antibiotics. Moreover, in our strains isolated from shellfish, we identified numerous virulence, antibiotic, and heavy metal resistance determinants, also additional to those previously found in the genome sequenced A. butzleri ...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
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Source: Frontiers in cellular and infection microbiology - Category: Microbiology Source Type: research
Abstract We used a green sol-gel synthesis method to fabricate a novel nanoporous copper aluminosilicate (CAS) material. Nanoporous CAS was characterized using X-ray powder diffraction (XRD), field emission transmission and scanning electron microscopies (FE-TEM/FE-SEM), Fourier transform infrared (FTIR) spectroscopy, and optical analyses. The CAS was also evaluated for use as a promising disinfectant for the inactivation of waterborne pathogens. The antimicrobial action and minimum inhibitory concentration (MIC) of this CAS disinfectant were determined against eight microorganisms (Escherichia coli, Salmonella en...
Source: Environmental Science and Pollution Research International - Category: Environmental Health Authors: Tags: Environ Sci Pollut Res Int Source Type: research
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Source: Colloids and Surfaces A: Physicochemical and Engineering Aspects - Category: Chemistry Source Type: research
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Source: Journal of Food Science - Category: Food Science Authors: Tags: Food Engineering, Materials Science, & Nanotechnology Source Type: research
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Source: Food Microbiology - Category: Food Science Authors: Tags: Food Microbiol Source Type: research
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