Blue Light Sensing in Listeria monocytogenes Is Temperature-Dependent and the Transcriptional Response to It Is Predominantly SigB-Dependent

In this study, the influence of temperature and growth phase on the response of L. monocytogenes to blue light was investigated and the global transcriptional response to blue light was elucidated using an RNAseq-based approach. Stationary phase cells were found to be significantly more resistant to killing by blue light (470 nm) than exponential phase cells. Temperature also had a marked effect on blue light resistance with cells cultured at 37°C being much more sensitive than cells grown at 30°C. The role of σB in light tolerance was confirmed but this effect was observed only at 30°C. σB activation by blue light was assessed by measuring the transcriptional response of known σB-dependent genes (sigB, lmo2230, and opuCA) to light. The transcripts were induced by blue light only at 30°C suggesting that blue light fails to activate σB at 37°C. The light-induced transcription at 30°C was dependent on a functional blue light sensor, Lmo0799 (which we rename herein as RsbL). A transcriptomic analysis of the response to sub-lethal levels of blue light found that the changes in transcription were almost entirely σB-dependent. A mutant where the light sensing mechanism of RsbL was inactivated through an amino acid substitution (Cys56Ala) was found to have an attenuated response to blue light, but residual activation of σB-dependent genes suggested that alternative routes for activation of σB by light are likely to ex...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research

Related Links:

White Castle has issued a voluntary recall of multiple products for possible listeria contamination. The recall affects a limited number of frozen six-pack cheeseburgers, frozen six-pack hamburgers, frozen six-pack pack jalapeno cheeseburgers, 16-pack hamburgers and 16-pack cheeseburgers, according to the US Food and Drug Administration.
Source: CNN.com - Health - Category: Consumer Health News Source Type: news
Fast-food chain says bacteria that can cause serious infection linked to one of its manufacturing plants.
Source: Health News: CBSNews.com - Category: Consumer Health News Source Type: news
The White Castle recall is for 6-pack cheeseburgers, 6-pack hamburgers, 6-pack jalapeno cheeseburgers, 16-pack hamburgers, and 16-pack cheeseburgers with best by dates ranging from 04 Aug 2020 to 17 Aug 2020.
Source: WebMD Health - Category: Consumer Health News Source Type: news
White Castle has issued a voluntary recall of five varieties of its frozen sliders after a third-party laboratory found listeria in a sample from one of the company's manufacturing facilities.
Source: the Mail online | Health - Category: Consumer Health News Source Type: news
Fast-food chain says bacteria that can cause serious infection linked to one of its manufacturing plants.
Source: Health News: CBSNews.com - Category: Consumer Health News Source Type: news
White Castle has initiated a voluntary recall of a limited number of frozen 6 pack cheeseburgers, frozen 6 pack hamburgers, frozen 6 pack jalapeno cheeseburgers, and 16 pack hamburgers, 16 pack cheeseburgers for the possible presence of Listeria monocytogenes.
Source: FDA Food Safety - Category: Food Science Authors: Source Type: alerts
They may take our broccoli florets, our chicken, and our sausage products, but they will never take our sushi. Well, actually they can.
Source: Forbes.com Healthcare News - Category: Pharmaceuticals Authors: Source Type: news
They may take our broccoli florets, our chicken, and our sausage products, but they will never take our sushi. Well, actually they can.
Source: Forbes.com Healthcare News - Category: Pharmaceuticals Authors: Source Type: news
CONCLUSION: Infections caused by L. monocytogenes were uncommon but could cause severe sepsis and mortality, especially in susceptible populations. Our study focused on neurological involvement and severe invasive forms of listeriosis. Neuroinvasive forms were the most important risk factors for severe illness but not for mortality. PMID: 31791803 [PubMed - as supplied by publisher]
Source: Medicina Clinica - Category: General Medicine Tags: Med Clin (Barc) Source Type: research
Products are sold in 31 states and Washington, D.C., by Trader Joe's, 7-Eleven, Walgreens and other retailers.
Source: Health News: CBSNews.com - Category: Consumer Health News Source Type: news
More News: Genetics | Listeria | Microbiology | Study