Multiplexed Digital Detection of B-ALL Leukemia Fusion Transcripts using the NanoString nCounter System

In this study, we used nanoString technology to design, validate, and evaluate a multiplex panel for detection of B-ALL fusion transcripts. Fifty-one B-ALL fusion transcripts reported in children in the literature were included in the design of the nanoString panel. Twenty-six fusion transcripts were validated using 64 positive control samples and 74 negative control samples with 100% sensitivity and 99% in comparison to Reverse Transcription-PCR.

Source: Journal of Molecular Diagnostics - November 12, 2019 Category: Pathology Authors: Yunan Zhong, Kassa Beimnet, Zaman Alli, Anthony Arnoldo, Paul E. Kowalski, Gino R. Somers, Cynthia Hawkins, Mohamed Abdelhaleem Source Type: research