Endogenous Annexin-A1 Negatively Regulates Mast Cell-Mediated Allergic Reactions

Mast cell stabilizers like cromoglycate and nedocromil are mainstream treatments for ocular allergy. Biochemical studies in vitro suggest that these drugs prevent mast cell degranulation through the release of Annexin-A1 (Anx-A1) protein. However, the direct effect of Anx-A1 gene deletion on mast cell function in vitro and in vivo is yet to be fully investigated. Hence, we aim to elucidate the role of Anx-A1 in mast cell function, both in vivo and in vitro, using a transgenic mouse model where the Anx-A1 gene has been deleted. Bone marrow-derived mast cells (BMDMCs) were cultured from wild-type animals and compared throughout their development to BMDMCs obtained from mice lacking the Anx-A1 gene. The mast cell differentiation, maturity, mediator, and cytokine release were explored using multiple biochemical techniques, such as Western blots, ELISA, and flow cytometry analysis. Electron microscopy was used to identify metachromatic granules content of cells. For in vivo studies, Balb/C wild-type and Anx-A1-deficient mice were divided into the following groups: group 1, a control receiving only saline, and group 2, which had been sensitized by prior exposure to short ragweed (SRW) pollen by topical contact with the conjunctival mucosae. Allergic conjunctivitis was evaluated blind after 24 h by trained observers scoring clinical signs. Electron micrographs of BMDMCs from Anx-A1-null mice revealed more vacuoles overall and more fused vacuoles than wild-type cells, suggesting enha...
Source: Frontiers in Pharmacology - Category: Drugs & Pharmacology Source Type: research

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Subcutaneous immunotherapy (SCIT) is effective for allergic rhinitis and conjunctivitis, asthma, and insect venom hypersensitivity. The risk of severe allergic reactions induced by SCIT remains low, and mild systemic reactions have recently shown a tendency to decline. However, near-fatal and fatal anaphylactic reactions may occur. Clinicians administering allergen-specific immunotherapy should receive specialized training and be aware of risk factors and preventive measures to avoid severe allergic reactions induced by SCIT.
Source: Immunology and Allergy Clinics of North America - Category: Allergy & Immunology Authors: Source Type: research
Conclusions: The frequency of LAR in asthmatic subjects allergic to dust mite exposed in ALYATEC EEC was higher than in asthmatics sensitized to cat. Our results confirmed previous results with individual bronchial challenge. Therefore, the mite allergic asthma is a good model to study asthma pathophysiology and treatment.
Source: European Respiratory Journal - Category: Respiratory Medicine Authors: Tags: Allergy and immunology Source Type: research
Abstract Ocular allergy (OA), interchangeably known as allergic conjunctivitis, is a common immunological hypersensitivity disorder affecting up to 40% of the population. OA has been increasing in frequency with symptoms of itching, redness, and swelling that significantly impacts an individual's quality of life (QOL). OA is an often underdiagnosed and under-treated health problem as only 10% of patients with OA symptoms seek medical attention while the majority of patients manage with over the counter medications and complementary nonpharmacological remedies. The clinical course, duration, severity, and co-morbid...
Source: Annals of Allergy, Asthma and Immunology - Category: Allergy & Immunology Authors: Tags: Ann Allergy Asthma Immunol Source Type: research
Ocular allergy (OA), interchangeably known as allergic conjunctivitis, is a common immunological hypersensitivity disorder affecting up to 40% of the population. OA has been increasing in frequency with symptoms of itching, redness, and swelling that significantly impacts an individual ’s quality of life (QOL). OA is an often underdiagnosed and under-treated health problem as only 10% of patients with OA symptoms seek medical attention while the majority of patients manage with over the counter medications and complementary nonpharmacological remedies.
Source: Annals of Allergy, Asthma and Immunology - Category: Allergy & Immunology Authors: Tags: Special Series Source Type: research
Conclusions The long-term risks of autoimmune disorders are significantly higher in patients with allergic diseases. Allergic diseases and autoimmune disorders show age- and sex-related clustering patterns.
Source: European Respiratory Journal - Category: Respiratory Medicine Authors: Tags: Asthma and allergy Original Articles: Asthma and allergy Source Type: research
Severe allergic conjunctivitis (AC) can lead to debilitating ocular symptoms with a risk of vision loss. Driven primarily by conjunctival mast cells and eosinophils, AC is also commonly associated with atopic conditions such as atopic dermatitis, asthma, and rhinitis. AC can present in various forms including atopic keratoconjunctivitis (AKC), vernal keratoconjunctivitis (VKC), and perennial allergic conjunctivitis (PAC). AK002 is an anti-Siglec-8 antibody that selectively depletes eosinophils and inhibits mast cells.
Source: Annals of Allergy, Asthma and Immunology - Category: Allergy & Immunology Authors: Source Type: research
Ragweed sublingual immunotherapy (SLIT)-tablet improves symptoms and decreases rescue medication use in adults with allergic rhinitis with or without conjunctivitis (AR/C) but has not been evaluated in children. This international, double-blind, placebo-controlled trial evaluated the efficacy and safety of ragweed SLIT-tablet in children with AR/C.
Source: Annals of Allergy, Asthma and Immunology - Category: Allergy & Immunology Authors: Tags: Rhinitis, Other Upper Airway Disorders Source Type: research
We have previously shown that prescription opioid analgesics for chronic pain are associated with increased coding for allergic rhino-conjunctivitis (ARC) and asthma. As GABA agonists are increasingly used for chronic pain and have immunomodulatory effects, we investigated if their use is associated with ARC and asthma.
Source: Annals of Allergy, Asthma and Immunology - Category: Allergy & Immunology Authors: Source Type: research
We describe a case of allergic conjunctivitis with progressive unilateral eye symptoms.
Source: Annals of Allergy, Asthma and Immunology - Category: Allergy & Immunology Authors: Source Type: research
While ocular disorders, including allergic conjunctivitis, are common in patients with atopic dermatitis, studies have suggested that dupilumab-treated patients have an increased conjunctivitis risk.The British Journal of Dermatology
Source: Medscape Allergy Headlines - Category: Allergy & Immunology Tags: Dermatology Journal Article Source Type: news
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