Human iPSC differentiation to retinal organoids in response to IGF1 and BMP4 activation is line ‐ and method‐dependent

Three human iPSC lines were differentiated to retinal organoids using differentiation protocols activating either BMP4 or IGF1 signalling pathways. All cell lines were able to generate retinal organoids. However, the two differentiation approaches produced bias toward certain retinal cell types within the organoids, which was iPSC line ‐ and method‐dependent. AbstractInduced pluripotent stem cell (iPSC) ‐derived retinal organoids provide a platform to study human retinogenesis, disease modelling and compound screening. Whilst retinal organoids may represent tissue structures with greater physiological relevance to thein vivo human retina, their generation is not without limitations. Various protocols have been developed to enable development of organoids with all major retinal cell types, however variability across iPSC lines is often reported. Modulating signalling pathways important for eye formation, such as those involving bone morphogenetic protein 4 (BMP4) and insulin ‐like growth factor (IGF1), is a common approach used for the generation of retinal tissuein vitro. We used three human iPSC lines to generate retinal organoids by activating either BMP4 or IGF1 signalling and assessed differentiation efficiency by monitoring morphological changes, gene and protein expression, and function. Our results showed that iPSC ability to give rise to retinal organoids in response to IGF1 and BMP4 activation was line ‐ and method‐dependent. This demonstrates that carefu...
Source: Stem Cells - Category: Stem Cells Authors: Tags: Embryonic Stem Cells/Induced Pluripotent Stem Cells Source Type: research