KLF2+ stemness maintains human mesenchymal stem cells in bone regeneration

This study provides insight into the stemness‐directed synergistic interaction between hMSCs and HUVECs, and our results will have a profound impact on further strategies involving the applicatio n of KLF2+ hMSC/HUVEC ‐laden GelMA hydrogel in vascular network bioengineering and bone regeneration.© AlphaMed Press 2019Significance StatementKLF2 was creatively put forward as a novel marker in vitro for MSCs to investigate the osteogenesis and angiogenesis between KLF2+ MSCs and ECs. We demonstrated there was a synergistic strategy to help readers understand the process of intercellular interaction between hMSCs and HUVECs, which KLF2+ hMSCs secreted angiogenic factors like ANG1 initially, and some of hMSCs then differentiated into pericytes through PDGF ‐BB/PDGFR‐ß signaling pathway. Both of which improved the formation of blood vessels. Matured HUVECs in turn synergistically enhanced the osteogenesis of KLF2+ hMSCs through the up‐regulated VEGF more efficiently. This work therefore focuses on the synergistic strategy between KLF2+ hMSCs and HUVECs and will have a profound impact on further vascular network bioengineering and bone regeneration.

Source: Stem Cells - November 12, 2019 Category: Stem Cells Authors: Ying Zhou, Chao Liu, Jianxiang He, Lingqing Dong, Huiyong Zhu, Bin Zhang, Xiaoxia Feng, Wenjian Weng, Kui Cheng, Mengfei Yu, Huiming Wang Tags: Regenerative Medicine Source Type: research