Organic Acids Produced during Fermentation and Sensory Perception in Specialty Coffee using Yeast Starter Culture

This study aimed to demonstrate how the organic acids and volatile profiles were impacted by coffee fermentation using four starter cultures (Meyerozyma caribbica (CCMA 0198), Saccharomyces cerevisiae (CCMA 0543), Candida parapsilosis (CCMA0544), and Torulaspora delbrueckii (CCMA 0684)) inoculated in two varieties of coffee (Bourbon Amarelo and Canário Amarelo) using natural and pulped natural processing methods and sensory perception. Real-time PCR (qPCR) was used to verify the dynamic behavior of yeast populations. Organic acids were detected using high performance liquid chromatography (HPLC) and gas chromatography–mass spectrometry (GC–MS) was used to detected volatile compounds. Sensory analysis wasperformed on the roasted coffee. Citric, malic, succinic, lactic, oxalic, isobutyric, and propionic acids and 105 volatile compounds were detected. At the beginning of fermentation, treatments with natural processing presented higher number of volatiles compounds. After fermentation, the main compounds groups were acids, alcohols, and aldehydes. The perception of sensory attribute (fruity, nutty, cocoa) varied with the coffee variety, type of processing, and type of inoculum. The use of yeasts is an alternative for sensorial differentiation of coffee variety Canário Amarelo and Bourbon Amarelo. The stainless-steel containers showed good results for coffee fermentation.Graphical abstract
Source: Food Research International - Category: Food Science Source Type: research

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In conclusion, the results suggest that, because of its bioactivity including the antioxidant, antimicrobial, and enzyme inhibition properties, the D. macrolepis can be accepted as a promising and natural source for the industrial applications. The present study is the first study, in which the bioactive components and the antioxidant, antimicrobial, and enzyme inhibition properties of endemic D. macrolepis plant were determined.
Source: Saudi Pharmaceutical Journal - Category: Drugs & Pharmacology Source Type: research
o-Cadavid E Abstract The biotechnological potential of yeasts associated to different habitats in Colombia has been poorly studied, especially the yeasts associated with different plant structures. Fruit pulps are interesting substrates mainly for the growth of yeast species, that can positively affect the productivity and quality of some bioeconomic species. Therefore, the objective of this study was to identify the dominant yeast species associated with mango and rose apple fruit pulps in Cali, Colombia. A total of 90 isolates were obtained, which were grouped considering their colony morphology. The D1/D2 domai...
Source: An Acad Bras Cienc - Category: Science Authors: Tags: An Acad Bras Cienc Source Type: research
Publication date: Available online 5 December 2019Source: Food Research InternationalAuthor(s): Mari Uyeda, Gustavo Bueno Duarte, Anna Maria Alves de Piloto Fernandes, Fabiano Jares Contesini, Márcia Cristina Fernandes Messias, Gabrielle Kristine Doratiotto de Santis, Karina Vieira Barros, Ana Valéria Colnaghi Simionato, Patricia de Oliveira CarvalhoAbstractBoth preventive and curative therapies have created a considerable demand for n-3 PUFAs (polyunsaturated fatty acids) from fish oil, such as eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids, for human use. Bio-synthesized sardine oil (bioSO) concentr...
Source: Food Research International - Category: Food Science Source Type: research
Publication date: Available online 6 December 2019Source: Comparative Biochemistry and Physiology Part C: Toxicology &PharmacologyAuthor(s): Trine Grabau Jensen, Martin Holmstrup, René Bjerregaard Madsen, Marianne Glasius, Lam Ngoc Trac, Philipp Mayer, Bodil Ehlers, Stine SlotsboAbstractVolatile monoterpenes are emitted in large quantities to both air and soil by many plant species. While studies have addressed effects of monoterpenes on aboveground invertebrates, we have much poorer understanding of the possible effects of monoterpenes on soil invertebrates. Monoterpenes play a protective role in some plant spe...
Source: Comparative Biochemistry and Physiology Part C: Toxicology and Pharmacology - Category: Toxicology Source Type: research
Conclusions: Optimal efficacy of amphotericin B against C. albicans is achieved in cold storage conditions at concentrations ≥1.25 μg/mL, and 2.5 μg/mL reduces Candida contamination by>90% after 6 hours of cold storage without sacrificing CEC health.
Source: Cornea - Category: Opthalmology Tags: Basic Investigation Source Type: research
ConclusionsThe presence of Candida spp airway colonization is associated with longer durations of mechanical ventilation, higher 28-day mortality, higher ICU mortality, and probably longer ICU length of stay compared with the absence of colonization in patients with VAP.
Source: American Journal of Infection Control - Category: Infectious Diseases Source Type: research
Publication date: Available online 6 December 2019Source: The Journal of Prosthetic DentistryAuthor(s): Worachat Namangkalakul, Sunpatch Benjavongkulchai, Teeraphat Pochana, Alitta Promchai, Wuttika Satitviboon, Sukanya Howattanapanich, Rawi Phuprasong, Nicha Ungvijanpunya, Danaiya Supakanjanakanti, Tatcha Chaitrakoonthong, Sureeporn Muangsawat, Panida Thanyasrisung, Oranart MatangkasombutAbstractStatement of problemCandida adherence to the denture base is an important cause of denture stomatitis. In addition, infections with drug-resistant Candida have become more prevalent, especially among elderly and immunocompromised ...
Source: The Journal of Prosthetic Dentistry - Category: Dentistry Source Type: research
In this study, the co-immobilization of cellobiose dehydrogenase (CDH) and deoxyribonuclease I (DNase) on positively charged chitosan nanoparticles (CSNPs) resulted in a bi-functional nanoparticle (CSNP-DNase-CDH) targeting both biofilm matrix and microorganisms. The in-vitro antibiofilm activities of CSNPs against monomicrobial and polymicrobial biofilms of Candida albicans and Staphylococcus aureus were evaluated. The results showed that CSNPs were able to penetrate across the matrix of biofilms and interfere with embedded microbial cells. CSNP-DNase-CDH exhibited a higher activity than CSNPs loaded with only DNase or CD...
Source: Materials Science and Engineering: C - Category: Materials Science Source Type: research
Publication date: Available online 2 December 2019Source: Journal of Molecular StructureAuthor(s): R. Rama, S. MeenakshiAbstractA novel Aliquat 336 based functionalized ionic liquid, methyltrioctylammonium naphthylacetate ([A336]+[NAA]-) have been synthesized and characterized using IR, UV, 1H NMR and Raman spectroscopy techniques. The prepared IL was tested for antimicrobial properties and chromium (VI) extraction. Anti-bacterial test was carried out towards five types of bacteria namely Staphylococcus aureus, Escherichia coli, Pseudomonas aeroginosa, Bacillus subtilis and Salmonella typhi. The antifungal test was done ou...
Source: Journal of Molecular Structure - Category: Molecular Biology Source Type: research
Publication date: December 2019Source: European Polymer Journal, Volume 121Author(s): Wan-Xia Wu, Jun Li, Xian-Ling Yang, Na Wang, Xiao-Qi YuAbstractHigh-molecular-weight renewable semicrystalline poly(β-thioether ester) with acid-labile β-thiopropionate groups was synthesized via Candida antarctica lipase B (CALB)-catalyzed polycondensation of methyl 3-((11-hydroxyundecyl)thio)propanoate (MHUTP) derived from castor oil. The monmer was obtained through thiol-ene click chemistry. The influences of polymerization conditions such as reaction temperature and time on the molecular weight were studied. Copolymerization...
Source: European Polymer Journal - Category: Chemistry Source Type: research
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