Critical review on proteotypic peptide marker tracing for six allergenic ingredients in incurred foods by mass spectrometry

Publication date: Available online 13 November 2019Source: Food Research InternationalAuthor(s): Rosa Pilolli, Chiara Nitride, Nathalie Gillard, Anne- Catherine Huet, Christof van Poucke, Marc de Loose, Olivier Tranquet, Collette Larré, Karine Adel-Patient, Hervé Bernard, E.N. Clare Mills, Linda MonaciAbstractPeptide marker identification is one of the most important steps in the development of a mass spectrometry (MS) based method for allergen detection, since the robustness and sensitivity of the overall analytical method will strictly depend on the reliability of the proteotypic peptides tracing for each allergen. The European legislation in place issues the mandatory labelling of fourteen allergenic ingredients whenever used in different food formulations. Among these, six allergenic ingredients, namely milk, egg, peanut, soybean, hazelnut and almond, can be prioritized in light of their higher occurrence in food recalls for undeclared presence with serious risk decision.In this work, we described the results of a comprehensive evaluation of the current literature on MS-based allergen detection aiming at collecting all available information about proteins and peptide markers validated in independent studies for the six allergenic ingredients of interest. The main features of the targeted proteins were commented reviewing all details available about known isoforms and sequence homology particularly in plant-derived allergens. Several critical aspects affecting peptide ma...
Source: Food Research International - Category: Food Science Source Type: research