Bio-assay of Acintobacter baumannii using DNA conjugated with gold nano-star: A new platform for microorganism analysis

In this study, gold nano-star was synthesized and used for bio-conjugation with pDNA toward the detection of target sequences. Synthesized probe (5ʹ TTG TGA ACT ATT TAC GTC AGC ATG C3ʹ) of Acinetobacter baumannii was found with excellent sensitivity. After the hybridization of pDNA with cDNA, target DNA (5ʹ GCA TGC TGA CGT AAA TAG TTC ACA A 3ʹ) was easily measured. According to ultra-sensitivity of the engineered optical DNA-based bio-assay, it is potentially applied in the bacterial detection of the environmental and clinical specimens. Here, the selection of engineered biosensor in the presence of two mismatch sequences was investigated. The results indicated an acceptable choice for DNA-based assays. The low limit of quantification (LLOQ) of genosensor was obtained as 1 fM. The present study is a very important diagnostic examination to recognize Acinetobacter baumannii, which can be a best alternative to the traditional methods.Graphical abstract
Source: Enzyme and Microbial Technology - Category: Biotechnology Source Type: research

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CONCLUSION: Enterococcus spp. with susceptibility to limited antibiotics was one of the main pathogens in Korean IAIs, along with E. coli and Klebsiella spp., which were highly susceptible to imipenem, amikacin, and piperacillin/tazobactam. Meanwhile, the low susceptibilities of E. coli or Klebsiella spp. to amoxicillin/clavulanate, advanced-generation cephalosporins, and ciprofloxacin should be considered when determining empirical antibiotic therapy in clinical practice. PMID: 31808326 [PubMed - in process]
Source: Journal of Korean Medical Science - Category: Biomedical Science Tags: J Korean Med Sci Source Type: research
In this study, we use 16S and 18S rRNA Illumina MiSeq sequencing data to characterize and compare the microbiota associated with fresh and strong flavor style flue ‐cured tobacco leaves and reveal correlations between the microbial taxa within them. Flue‐cured tobacco leaf microbiota are correlated with flavor style, and species present at low numbers within the microbial communities can be used as microbial markers to discriminate between samples of diffe rent flavor styles and to stabilize flue‐cured tobacco leaf microbial communities. AbstractFermentation, also known as aging, is vital for enhancing the quality of...
Source: MicrobiologyOpen - Category: Microbiology Authors: Tags: ORIGINAL ARTICLE Source Type: research
Authors: Bassetti M, Giacobbe DR PMID: 31790306 [PubMed - as supplied by publisher]
Source: Expert Opinion on Pharmacotherapy - Category: Drugs & Pharmacology Tags: Expert Opin Pharmacother Source Type: research
ConclusionThe multicomponent intervention performed by a multidisciplinary team was effective to eradicate the endemic MDR-Ab.
Source: Antimicrobial Resistance and Infection Control - Category: Infectious Diseases Source Type: research
Acinetobacter baumannii is an important clinical pathogen which often causes fatal infections among seriously ill patients. Treatment options for managing infections caused by this organism have become limited as a result of emergence of carbapenem resistant strains. In the current study, whole genome sequencing, gene expression studies and enzyme kinetics analyses were performed to investigate the underlying carbapenem resistance mechanisms in fourteen clinical A. baumannii strains isolated from two hospitals, one each in Hong Kong and Henan Province, People’s Republic of China. A large majority of the A. baumannii ...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Conclusion:Acinetobacter is commonly isolated Lebanon. In view of the spread of resistance among these isolated and their dissemination, Infection control measures attempting to control the spread of this genus in and outside hospitals are lacking and thus require more attention and stewardship activities.
Source: The Journal of Infection in Developing Countries - Category: Infectious Diseases Source Type: research
In this study, we investigated the bacterial pathogen community in 88 sites at rivers around Lake Chaohu Basin, China, using Illumina miseq sequencing. The results showed that three opportunistic pathogens: Acinetobacter, Massilia, and Brevundimonas, were the three abundant bacterial genera in all samples, and had a relative abundance of 0.33 to 49.28% (average 8.80%), 0.06 to 25.4% (average 4.6%), 0.01 to 12.82% (average 2.6%) of all bacterial sequences, respectively. Our results indicated that a high abundance of opportunistic pathogens was observed in the rivers of the Lake Chaohu Basin, and that effective treatment and...
Source: International Journal of Environmental Research and Public Health - Category: Environmental Health Authors: Tags: Communication Source Type: research
Abstract Recently, two novel plasmid-mediated tigecycline resistance genes, tet(X3) and tet(X4), have been identified in Acinetobacter baumannii and Escherichia coli, respectively, both of which can significantly compromise the efficacy of tigecycline (1-3), which is one of the few available drugs that can be used to treat infections caused by extensively drug-resistant pathogens (4).…. PMID: 31791944 [PubMed - as supplied by publisher]
Source: Antimicrobial Agents and Chemotherapy - Category: Microbiology Authors: Tags: Antimicrob Agents Chemother Source Type: research
Abstract A novel bacterial strain, named S23T, was isolated from chicken meat of local market in Korea. Cells were Gram-negative, milky-yellow colored, non-motile and coccobacillus. The strain was obligate aerobic and catalase-positive, oxidase-negative, optimum growth temperature and pH were 25 °C and pH 7.0, respectively. On the basis of 16S rRNA gene sequence analysis, strain S23T belongs to the genus Acinetobacter and is most closely related to Acinetobacter defluvii KCTC 52503 T (97.40%). The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) value between strain S23T and...
Source: Archives of Microbiology - Category: Microbiology Authors: Tags: Arch Microbiol Source Type: research
We hereby report a case of subclinical community ‐acquiredAcinetobacter pneumonia in association with a mature cystic teratoma masquerading as a lung abscess. A 22 ‐year‐old lady presented with fever and right pleuritic chest pain with chest X‐ray showing a right suprahilar shadow. Her symptoms largely subsided with antibiotic therapy but the shadow remained unresolved. Computerized tomography (CT) scan of thorax showed consolidation over the anterior se gment of the right upper lobe with a hypodense area suspicious of a lung abscess. Fine needle aspirates of the lesion grewAcinetobacter baumannii twice but the sha...
Source: Respirology Case Reports - Category: Respiratory Medicine Authors: Tags: Case Report Source Type: research
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