Melatonin and insulin modulates the cellular biochemistry, histoarchitecture and receptor expression during hepatic injury in diabetic rats

Publication date: Available online 12 November 2019Source: Life SciencesAuthor(s): Younis Ahmad Hajam, Seema RaiAbstractAimsThe present study was designed to ameliorate the integrated efficacy of exogenous melatonin and insulin on tissue biochemical, serological, histopathological architecture and receptor expression of melatonin (MT1, MT2) and insulin receptor (IR) expression against the hepatic injury in diabetic rats. Materials and Method; the rats were randomly allocated into nine different experimental groups. Diabetes was induced by streptozotocin (15mg/kg) for 6 days. Rats having blood glucose level above 250mg/dl were considered as diabetic. Animals euthanized after 4 weeks, blood and liver samples were collected to perform various biochemical, serological, histopathological and receptor expression of melatonin (MT1, MT2) and insulin receptor (IR). Key findings; Diabetic rats revealed significant increase in lipid peroxidation (LPO) of liver tissue, liver function tests (ALT, AST and ALP), increase in serum cholesterol, LDL, VLD, but decrease in HDL level. Further, diabetic rats exhibited significant decrement in antioxidative enzymatic system (GSH, SOD, CAT, GR, GPX, G6PDH and GST), total tissue protein and glycogen content. Histomicrograph of liver of diabetic rats resulted in vacuolization indicating cellular damages as well as upregulation in liver MT1, MT2 and IR protein expression. However, the combined therapy (Melatonin and insulin treatment) revealed signific...
Source: Life Sciences - Category: Biology Source Type: research

Related Links:

Publication date: Available online 6 December 2019Source: Diabetes &Metabolic Syndrome: Clinical Research &ReviewsAuthor(s): Biswapriya B. Misra, Anoop MisraAbstractT2DM, variously known as diabetes mellitus type 2, adult-onset diabetes; noninsulin-dependent diabetes mellitus (NIDDM) is a global silent killer, with> 450 million adults affected worldwide. A diverse array of non-modifiable risk factors such as family history, age (>45 yrs), race/ethnicity, and history of gestational diabetes) and modifiable risk factors such as physical inactivity, high body fat or body weight, high blood pressure and high chol...
Source: Diabetes and Metabolic Syndrome: Clinical Research and Reviews - Category: Endocrinology Source Type: research
The complete updated Wheat Belly program is now available in the Revised &Expanded Edition of Wheat Belly! (For availability, see below.) In the original Wheat Belly, I recounted how agribusiness and geneticists altered traditional strains of wheat, yielding the high-yield semi-dwarf strain that now dominates supermarket shelves. Consuming modern foods made from this plant yields peculiar, sometimes crippling, health effects, while dramatic benefits develop upon removing it from the diet. If you’ve been following the Wheat Belly conversations, you are no stranger to the huge successes people have shared on t...
Source: Wheat Belly Blog - Category: Cardiology Authors: Tags: Open autoimmune bowel flora cholesterol diabetes gluten-free grain-free joint pain microbiota prebiotic probiotic Thyroid Weight Loss wheat belly Source Type: blogs
A sizable body of evidence, both mechanistic and epidemiological, supports the idea that exercise slows age-related cognitive decline. The report here is an example of the type, noting the results of a study in which some of the participants were assigned to an exercise program. The exercising participants exhibited a slower decline in cognitive function, particularly memory, in comparison to the others. This is a representative result: in general, the consensus in the scientific literature is that regular exercise is beneficial to cognitive function over the long term. Researchers theorized that the healthy lifes...
Source: Fight Aging! - Category: Research Authors: Tags: Daily News Source Type: blogs
ConclusionsThere was a significant association between statin use and TUG inability in older Chinese inpatients with diabetes.
Source: The Journal of Nutrition, Health and Aging - Category: Nutrition Source Type: research
euwenhuijsen Manuel Franco The aim of this study is to evaluate the relationship between the density of green spaces at different buffer sizes (300, 500, 1000 and 1500 m) and cardiovascular risk factors (obesity, hypertension, high cholesterol, and diabetes) as well as to study if the relationship is different for males and females. We conducted cross-sectional analyses using the baseline measures of the Heart Healthy Hoods study (N = 1625). We obtained data on the outcomes from clinical diagnoses, as well as anthropometric and blood sample measures. Exposure data on green spaces density at different buffer sizes wer...
Source: International Journal of Environmental Research and Public Health - Category: Environmental Health Authors: Tags: Article Source Type: research
The Pharmacogenomics Journal, Published online: 05 December 2019; doi:10.1038/s41397-019-0125-xStatin-induced LDL cholesterol response and type 2 diabetes: a bidirectional two-sample Mendelian randomization study
Source: The Pharmacogenomics Journal - Category: Drugs & Pharmacology Authors: Source Type: research
Discussion and conclusion: MDE possesses substantial antidiabetic activity, especially in lipid disorder regulation. This suggests that the use of MDE can be generalized to broader pharmacological studies, such as obesity and hyperlipidaemia. PMID: 31794270 [PubMed - in process]
Source: Pharmaceutical Biology - Category: Drugs & Pharmacology Tags: Pharm Biol Source Type: research
Authors: Oinonen L, Koskela J, Eräranta A, Tikkakoski A, Kähönen M, Niemelä O, Mustonen J, Pörsti I Abstract Purpose: The underlying causes of primary hypertension are not fully understood. Evidence on the relation of plasma calcium concentration with blood pressure (BP) is inconsistent and relies largely on studies utilizing office BP measurements in populations using cardiovascular drugs. In many studies adjustment for confounders was not optimal. In this cross-sectional study we examined the association of plasma total calcium concentration with the haemodynamic determinants of blood pre...
Source: Blood Pressure - Category: Hematology Tags: Blood Press Source Type: research
High cholesterol is known to be one of the primary risk factors for heart disease, since it can contribute to plaque buildup in the arteries. But even though the U.S. Centers for Disease Control and Prevention recommends regular cholesterol testing starting around age 20, many Americans don’t give cholesterol—or heart disease, for that matter—much thought until later in life. A new modeling study published in the Lancet gives extra reason not to put off cholesterol screening and treatment. It confirms that high blood levels of “bad” (or non-HDL) cholesterol are associated with a greater risk o...
Source: TIME: Health - Category: Consumer Health News Authors: Tags: Uncategorized Research Source Type: news
Abstract We investigated whether low-dose phloretin served as daily dietary supplements could ameliorate diabetic atherosclerosis and the role of kruppel-like factor 2 (KLF2). HUVECs cultured in high glucose medium were treated with different concentrations of phloretin and KLF2 mRNA, and protein level was detected. Diabetes was induced using streptozotocin in Apoe-/- mice after which they were fed a high-cholesterol diet for 8 weeks. Diabetic mice injected with KLF2 shRNA-lentivirus or control virus were treated with 20 mg/kg phloretin. Glucose, lipid profile, aortic atheroma, and endothelial nitric oxide synthas...
Source: Bioscience, Biotechnology, and Biochemistry - Category: Biochemistry Authors: Tags: Biosci Biotechnol Biochem Source Type: research
More News: Biochemistry | Biology | Cholesterol | Diabetes | Endocrinology | Insulin | Liver | Melatonin | Study | Urology & Nephrology