LncRNA DSCAM-AS1 Promotes Proliferation, Migration and Invasion of Colorectal Cancer Cells via Modulating miR-144-5p/CDKL1

Publication date: Available online 12 November 2019Source: Life SciencesAuthor(s): Jiaping Pei, Wen Xiao, Danyan Zhu, Xiaowei Ji, Liping Shi, Xiaozhao DengAbstractAimsThis work is to explore the expression, function and mechanism of long non-coding RNA (lncRNA) DSCAM-AS1 in colorectal cancer (CRC).Materials and MethodsPaired CRC tissues/adjacent tissues were collected to examine their expression levels of DSCAM-AS1, miR-144-5p and CDKL1 by qRT-PCR; DSCAM-AS1 shRNA was transfected into HCT-116 and SW480 cells to establish cell models, whose proliferation was detected by CCK-8 and plate colony formation assays. Transwell assay was used to evaluate the migration and invasion of cancer cells. QRT-PCR and Western blot were adopted to analyze the changes in the expression levels of miR-144-5p and CDKL1; a luciferase reporter gene assay was performed to determine the regulatory relationship between miR-144-5p and DSCAM-AS1, as well as between miR-144-5p and CDKL1.Key findingsDSCAM-AS1 was significantly up-regulated in CRC samples, and its expression was positively correlated with the expression of CDKL1 and negatively correlated with the expression of miR-144-5p. After the transfection of DSCAM-AS1 shRNAs into cancer cells, the proliferative and metastatic ability of the cells was reduced. DSCAM-AS1 could reduce the expression level of miR-144-5p by binding with it. Additionally, CDKL1 was also validated as a target gene of miR-144-5p, and DSCAM-AS1 was shown to indirectly regulate ...
Source: Life Sciences - Category: Biology Source Type: research

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