Simple strategy for single-chain fragment antibody-conjugated probe construction

Publication date: Available online 13 November 2019Source: Life SciencesAuthor(s): Chen Xu, Xiang Chen, Mingjuan Yang, Xiaopeng Yuan, Aizhi Zhao, Hujing BaoAbstractAimsA combination of biomarker and instrument technology diagnosis methods, especially antigen-targeted imaging methods, is required to increase the accuracy of the diagnosis of cancer. Currently, the targeting efficiency is limited by the conjugation methods used for the conjugation of antibodies and imaging materials. Here, a simple strategy for the conjugation of a probe and a single-chain fragment antibody (scFv) that does not change the characteristics of the antibody was shown.Main MethodsAn ScFv was conjugated with superparamagnetic iron oxide (SPIO) or indocyanine green (ICG) via a linker by utilizing the reaction between cysteine and maleimide. The characterization of the probe was performed by flow cytometry, confocal imaging, optical imaging and magnetic resonance imaging (MRI).Key findingsAfter conjugation, the scFv retained high affinity, antigen specificity, and strong internalization ability. The application of the conjugated probe was also confirmed by optical imaging and MRI.SignificanceThe proposed strategy provides a simple method for the production of high efficiency antigen-targeted imaging probes for tumor diagnosis.
Source: Life Sciences - Category: Biology Source Type: research

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AbstractObjectiveTo report our experience with the use of intravoxel incoherent motion (IVIM) magnetic resonance imaging (MRI) and dynamic contrast-enhanced (DCE)-MRI in bone marrow before and after administration of granulocyte colony-stimulating factor (GCSF). Moreover, a small series of patients with bone metastases from breast cancer have been evaluated by IVIM DW-MRI and DCE-MRI before and after GCSF administration.Materials and methodsWe studied with IVIM-MRI and DCE-MRI 14 patients with rectal or uterine cervix cancer studied before and 4 –18 days after administration of GCSF; the second MR examination wa...
Source: La Radiologia Medica - Category: Radiology Source Type: research
Additional screening for women at high risk of breast cancer is commonplace in many countries, with evidence that magnetic resonance imaging (MRI) screening improves mortality.1 The age of commencement, methods, and frequency varies between countries. In this paper, the authors review high-risk screening within Ireland,2 where women are referred to hospital-based clinics for surveillance following assessment by a geneticist or by the hospital high-risk surveillance clinic. In contrast to the UK, clinical examination is recommended in addition to yearly breast MRI.
Source: Clinical Radiology - Category: Radiology Authors: Tags: Commentary Source Type: research
This study aimed to clarify the diagnostic performance of DWI for assessing LN metastasis compared with positron emission tomography (PET) in patients with esophageal squamous cell cancer (eSCC).MethodsSeventy-six patients with histologically proven eSCC who underwent curative esophagectomy without neoadjuvant treatment were reviewed retrospectively. Harvested LNs were divided into 1229 node stations with 94 metastases. Diagnostic abilities and prognostic significance were compared.ResultsIn a station-by-station evaluation, the sensitivity was higher in DWI than PET (67% vs. 32%,P  
Source: Esophagus - Category: Gastroenterology Source Type: research
Conclusion: By use of our nomogram, bone scans of men with prostate cancer can be avoided in a large proportion of men. PMID: 31805808 [PubMed - as supplied by publisher]
Source: Scandinavian Journal of Urology - Category: Urology & Nephrology Tags: Scand J Urol Source Type: research
Publication date: Available online 6 December 2019Source: European UrologyAuthor(s): Marloes van der Leest, Bas Israël, Rianne R.M. Engels, Jelle O. Barentsz
Source: European Urology - Category: Urology & Nephrology Source Type: research
To review surveillance magnetic resonance imaging (MRI) and clinical breast examinations (CBE) performed for women at high risk of breast cancer in order to determine recall and cancer-detection rates.
Source: Clinical Radiology - Category: Radiology Authors: Source Type: research
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Source: TIME: Health - Category: Consumer Health News Authors: Tags: Uncategorized medicine onetime Research syndication Source Type: news
This report describes the feasibility and short-term outcomes of an...
Source: BMC Urology - Category: Urology & Nephrology Authors: Tags: Research article Source Type: research
Authors: Kong TW, Son JH, Paek J, Chang SJ, Ryu HS Abstract OBJECTIVE: To evaluate oncologic outcomes of minimally invasive radical hysterectomy (RH) in early cervical cancer before and after the application of parametrial invasion (PMI) criterion on magnetic resonance imaging (MRI) and vaginal colpotomy (VC). METHODS: A total of 216 International Federation of Gynecology and Obstetrics stage IB-IIA cervical cancer patients who underwent minimally invasive RH was identified between April 2006 and October 2018. Patients were classified into the pre-PMI intracorporeal or VC (IVC) (n=117) and post-PMI VC groups (n...
Source: Journal of Gynecologic Oncology - Category: OBGYN Tags: J Gynecol Oncol Source Type: research
Publication date: Available online 18 November 2019Source: Best Practice &Research Clinical Obstetrics &GynaecologyAuthor(s): Elaine F. Harkness, Susan M. Astley, D.Gareth EvansAbstractThe incidence of breast cancer continues to increase worldwide. Population-based screening is available in many countries but may not be the most efficient use of resources, thus interest in risk-based/stratified screening has grown significantly in recent years. An important part of risk-based screening is the incorporation of mammographic density (MD) and single nucleotide polymorphisms (SNPs) into risk prediction models to be comb...
Source: Best Practice and Research Clinical Obstetrics and Gynaecology - Category: OBGYN Source Type: research
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