Determination of motolimod concentration in rat plasma by liquid chromatography-tandem mass spectrometry and its application in a pharmacokinetic study
In this study, a novel and sensitive liquid chromatography-tandem mass spectrometry method was developed for quantifying motolimod in rat plasma and subsequently used in a pharmacokinetic study. Proteins were precipitated from plasma samples using acetonitrile prior to the analysis. GS-9620 was used as an internal standard. High-performance liquid chromatography was performed using a Spursil C18-EP column (3 µm, 50 × 2.1 mm). Aqueous ammonium formate and acetonitrile were used as the mobile phase. Motolimod was detected using an electrospray ionization interface under multiple reaction monitoring conditions in the positive ion mode. The developed method produced a linear correlation over a concentration range of 1–1000 ng/mL (r = 0.9944). Intra- and inter-day precision values ranged from 4.8% to 10.7% (the lower limit of quantification precision value was 16.3%), whereas intra- and inter-day accuracy values ranged from 0.3% to 9.1%. The mean recovery of motolimod from rat plasma was 109.4%. Additionally, motolimod was found to be stable under various conditions (three freeze-thaw cycles, 6-h storage at room temperature, short- and long-term stability tests, and processing). The developed method was successfully used in a pharmacokinetic study in rats. Motolimod showed non-linear pharmacokinetics following its intravenous administration to rats at 0.6–6 mg/kg. Additionally, very low exposure (
No abstract available
Publication date: Available online 6 December 2019Source: Journal of American Association for Pediatric Ophthalmology and StrabismusAuthor(s): Weiyi Xia, Wen Wen, Xiying Wang, Chao Jiang, Lianqun Wu, Ling Ling, Jing Yao, Chen Zhao
Publication date: Available online 6 December 2019Source: Journal of American Association for Pediatric Ophthalmology and StrabismusAuthor(s): Peter Daniel, Martin Cogen
Publication date: Available online 7 December 2019Source: Case Reports in Women's HealthAuthor(s): Gordon Narayansingh, Jennifer HohAbstractAbdominal pain following in vitro fertilization (IVF) in the presence of enlarged ovaries commonly leads to the diagnosis of ovarian hyperstimulation syndrome (OHSS). Progressively worsening pain with a rise in inflammatory markers with normal hematological indices implies a diagnosis of ovarian torsion of the hyperstimulated ovary. Early recourse to laparoscopic assessment and derotation of the affected ovary even after 72 h following the onset of symptoms can allow a return to viab...
No abstract available
No abstract available
ABSTRACT: Intimate partner violence (IPV) occurs across all cultures, ethnicities, socioeconomic classes, and educational backgrounds. Nurses should be aware of IPV and causative factors that include personal attributes, stressors, and community issues. The Intimate Partner Exposome conceptual model is used to identify exogenous and endogenous IPV risk factors. In addition, distinctive environmental factors can increase risk for IPV. The purpose of this article is to increase recognition of causative factors and presenting signs to enhance the nurse's ability to identify and assist IPV victims.
This article discusses the experience of maternal loss and notes care needs of the motherless child-adult woman. Identification of women in this situation should be made as early as possible.
ABSTRACT: High mortality rates continue among African Americans related to complications of uncontrolled hypertension. The purpose of this study was to determine if a faith-based self-management education program would improve self-care activities related to the management of hypertension among African American adults. Ten African American adults who reported a diagnosis of hypertension for 6 months or longer completed an 8-week education program focused on strategies for hypertension management. The education program was enhanced with the utilization of spiritual components of prayer, Scripture reading, and journaling.
ABSTRACT: There has been an increase in student enrollment in advanced practice nurse programs, but limited information regarding attrition and dropout rates in nurse practitioner (NP) programs. Management of NP students includes student evaluations, preceptor guidance and support, student support, and use of reflective practice. Program directors, faculty members, and preceptors must assist NP students with role transitioning and help those who are not meeting the rigors of NP education to leave the program and discover alternative nursing roles.